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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 6,812 results for: mme

Showing: 221 - 240 of 6812 results

  1. Direct-seq_lentiGuide-Puro-Tetra Loop-8A8G

    Plasmid
    #157982
    Purpose
    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.
    Depositor
    Lijia Ma
    Article
    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:
    Insert
    S. pyogenes sgRNA cassette (Tetra-8A8G)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    May 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    157982-plasmid-map-sequence-id-332394

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  7. pEND-351_Cacnes_dCas9_CRISPRi

    Plasmid
    #225617
    Purpose
    Cutibacterium acnes replicative plasmid with dCas9 for CRISPRi. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Insert
    dCas9
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225617-plasmid-map-sequence-id-451418

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  10. pGBT-RP8.1

    Plasmid
    #189846
    Purpose
    Intron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Lens tagBFP frameshift 1.
    Depositor
    Stephen Ekker
    Article
    Ichino et al Elife. 2020 Aug 11;9. pii: 54572. doi: 1
    Type
    Empty backbone
    Use
    Cre/Lox
    Tags
    mRFP and tagBFP
    Expression
    Mutation
    Promoter
    beta Actin
    Available since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    189846-plasmid-map-sequence-id-373953

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  11. pGBT-RP2.2

    Plasmid
    #189842
    Purpose
    Intron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Ubiquitous GFP frameshift 2.
    Depositor
    Stephen Ekker
    Article
    Ichino et al Elife. 2020 Aug 11;9. pii: 54572. doi: 1
    Type
    Empty backbone
    Use
    Cre/Lox
    Tags
    GFP GM2 variant and mRFP
    Expression
    Mutation
    Promoter
    beta Actin
    Available since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    189842-plasmid-map-sequence-id-373952

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  12. pGBT-RP2.3

    Plasmid
    #189843
    Purpose
    Intron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Ubiquitous GFP frameshift 3.
    Depositor
    Stephen Ekker
    Article
    Ichino et al Elife. 2020 Aug 11;9. pii: 54572. doi: 1
    Type
    Empty backbone
    Use
    Cre/Lox
    Tags
    GFP GM2 variant and mRFP
    Expression
    Mutation
    Promoter
    beta Actin
    Available since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    189843-plasmid-map-sequence-id-373951

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  13. pGBT-RP8.3

    Plasmid
    #189848
    Purpose
    Intron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Lens tagBFP frameshift 3.
    Depositor
    Stephen Ekker
    Article
    Ichino et al Elife. 2020 Aug 11;9. pii: 54572. doi: 1
    Type
    Empty backbone
    Use
    Cre/Lox
    Tags
    mRFP and tagBFP
    Expression
    Mutation
    Promoter
    beta Actin
    Available since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    189848-plasmid-map-sequence-id-373955

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  16. pHis-TEV-Nsp15-E266Q

    Plasmid
    #218803
    Purpose
    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-E266Q (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag
    Depositor
    Debra Hansen
    Article
    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:
    Insert
    Nsp15-E266Q (N Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    TEV protease cleavable His6-tag
    Expression
    Bacterial
    Mutation
    E266Q (G to C)
    Promoter
    Available since
    May 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    218803-plasmid-map-sequence-id-433302

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  17. pHis-TEV-Nsp15-V66L

    Plasmid
    #219524
    Purpose
    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-V66L (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag
    Depositor
    Debra Hansen
    Article
    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:
    Insert
    Nsp15-V66L (N Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    TEV protease cleavable His6-tag
    Expression
    Bacterial
    Mutation
    V66L (G to C)
    Promoter
    Available since
    May 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    219524-plasmid-map-sequence-id-433377

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  18. pHis-TEV-Nsp15-A81V

    Plasmid
    #219525
    Purpose
    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-A81V (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag
    Depositor
    Debra Hansen
    Article
    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:
    Insert
    Nsp15-A81V (N Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    TEV protease cleavable His6-tag
    Expression
    Bacterial
    Mutation
    A81V (C to T)
    Promoter
    Available since
    May 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    219525-plasmid-map-sequence-id-433375

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  19. pHis-TEV-Nsp15-D183N

    Plasmid
    #219526
    Purpose
    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-D183N (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag
    Depositor
    Debra Hansen
    Article
    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:
    Insert
    Nsp15-D183N (N Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    TEV protease cleavable His6-tag
    Expression
    Bacterial
    Mutation
    D183N (G to A)
    Promoter
    Available since
    May 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    219526-plasmid-map-sequence-id-433305

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  20. pHis-TEV-Nsp15-H234A

    Plasmid
    #219529
    Purpose
    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-H234A (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag
    Depositor
    Debra Hansen
    Article
    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:
    Insert
    Nsp15-H234A (N Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    TEV protease cleavable His6-tag
    Expression
    Bacterial
    Mutation
    H234A (CAC to GCG)
    Promoter
    Available since
    May 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    219529-plasmid-map-sequence-id-433314

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Showing: 221 - 240 of 6812 results