-
Plasmid#68907PurposeNanoLuc expressed constiutively from PBT1311 and GH023 RBS, pNBU2 backbone, AmpRDepositorInsertNanoLuc
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 WT B-Myb
Plasmid#25965DepositorInsertB-Myb (MYBL2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPTK021-7-PARS
Plasmid#84990PurposeType 7, Pichia autonomously replicating sequenceDepositorInsertPichia autonomously replicating sequence
UseSynthetic BiologyTagsExpressionYeastMutationPromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNR220
Plasmid#79541PurposeInput plasmid: expresses three serine recombinases downstream of three inducible promoters.DepositorInsertsTP901
BxbI
A118
UseTagsHis tag and LAA degradation tagExpressionBacterialMutationPromoterAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMM656
Plasmid#68887PurposeRhamnose-inducible NanoLuc expression, pNBU2 backbone, AmpRDepositorInsertNanoLuc
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAT593
Plasmid#68909PurposeNanoLuc expressed constiutively from PBT1311 and rpiL* RBS, pNBU2 backbone, AmpRDepositorInsertNanoLuc
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMM597
Plasmid#68886PurposeIPTG-inducible NanoLuc expression, pNBU2 backbone, AmpRDepositorInsertsNanoLuc
LacI
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMM660
Plasmid#68889PurposeArabinogalactan-inducible NanoLuc expression, pNBU2 backbone, AmpRDepositorInsertNanoLuc
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAL-pck_NT
Plasmid#74069PurposepAL374 plasmid carrying the pck (NT) sgRNA, targeting the non-template strand of pck, SpecRDepositorInsertsgRNA pck (NT)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterptacAvailable sinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
Construct 12 - hUBCp_Cas9_3xNLS_p2a_puroR
Plasmid#81251PurposeLentiviral construct for building stable cell lines expressing Cas9 via puromycin selectionDepositorInsertCas9-3xNLS
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMM659
Plasmid#68888PurposeChondroitin sulfate-inducible NanoLuc expression, pNBU2 backbone, AmpRDepositorInsertNanoLuc
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTPGI_dCas9
Plasmid#64279Purposeencodes yeast-optimized dCas9 synthetic transcription factorDepositorInsertYeast-optimized dCas9
UseTagsSV40 NLSExpressionYeastMutationD10A & H840A relative to S. pyogenes Cas9PromoterpTPGIAvailable sinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAWp7
Plasmid#72730PurposepFUGW-UBCp-RFP-CMVp-GFPDepositorInsertUBCp-RFP-CMVp-GFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPTK020-7-attB
Plasmid#84989PurposeType 7, BxbI recognition siteDepositorInsertBxbI recognition site
UseSynthetic BiologyTagsExpressionYeastMutationBsaI site removed (1711 t to a)PromoterNonAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMM555
Plasmid#68880PurposeNanoLuc expressed constiutively from PcfxA, pNBU2 backbone, AmpRDepositorInsertNanoLuc
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPTK005-3a-αMF
Plasmid#84964PurposeType 3a, α-mating factor - secretion signalDepositorInsertα-mating factor secretion tag
UseSynthetic BiologyTagsExpressionYeastMutationPromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
Construct 33 - NFKBRp_Cas9_3xNLS_p2a-puroR
Plasmid#81255PurposeLentiviral construct for building stable cell lines expressing Cas9 in the presence of TNFa via puromycin selectionDepositorInsertCas9-3xNLS
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAL-pgi_NT
Plasmid#74067PurposepAL374 plasmid carrying the pgi (NT) sgRNA, targeting the non-template strand of pgi, SpecRDepositorInsertsgRNA pgi (NT)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterptacAvailable sinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAT892
Plasmid#68933PurposeInt9 expressed constitutively from PAM4 and the rpiL* RBS, pNBU2 backbone, AmpRDepositorInsertInt9
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAL-pyk_NT
Plasmid#74072PurposepAL374 plasmid carrying the pyk (NT) sgRNA targeting the non-template strand of pyk, SpecRDepositorInsertsgRNA pyk (NT)
UseCRISPRTagsExpressionBacterialMutationPromoterptacAvailable sinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only