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pYFP.LIC.HXGPRT

Plasmid

#83128

Purpose

Plasmid contains LIC cassette upstream of an in-frame copy of YFP and HXGPRT selectable marker cassette for endogenous tagging on the gene locus.

Depositor

Vernon Carruthers

Article

Huynh et al Eukaryot Cell. 2009 Apr;8(4):530-9. doi:

Insert

YFP

Use

Toxoplasma gondii

Tags

YFP

Expression

Mutation

Promoter

Endogenous Gene

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pJH2644

Plasmid

#179632

Purpose

Pnmr-1::TetanusToxin-UrSL-wCherry unc-54 3' UTR C.elegans AVA/E/D premoter IN and others expression of TetanusToxin-UrSL-wCherry

Depositor

Mei Zhen

Article

Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

Insert

TetanusToxin

Use

Tags

wCherry

Expression

Worm

Mutation

Promoter

Pnmr-1

Available since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-dfROX

Plasmid

#137170

Purpose

Lentiviral transfer vector for expression of roGFP2 and HyPer Red

Depositor

Shaun McCullough

Article

Faber et al Toxicol Sci. 2020 Jun 11. pii: 5856111.

Insert

roGFP2-2A-HyPer Red

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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pFU-MVIIA-PC

Plasmid

#24148

Depositor

Ines Ibanez-Tallon

Article

Auer et al Nat Methods. 2010 Feb 7. ():.

Insert

omega-Conotoxin MVIIA-FLAG-PDGF_R_TM-mCherry (PDGFRB Human, Conus magus, Aequorea victoria)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2010

Availability

Academic Institutions and Nonprofits only

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pFU-MVIIA-PE

Plasmid

#24144

Depositor

Ines Ibanez-Tallon

Article

Auer et al Nat Methods. 2010 Feb 7. ():.

Insert

omega-Conotoxin MVIIA-FLAG-PDGF_R_TM-EGFP (PDGFRB Human, Conus magus, Aequorea victoria)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2010

Availability

Academic Institutions and Nonprofits only

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pQL47 EGFP-VAMP-GST

Plasmid

#122971

Purpose

Reporter of botulinum toxin B cleavage

Depositor

Chandra Tucker

Article

Liu et al Neuron. 2019 Jan 15. pii: S0896-6273(19)

Insert

EGFP-VAMP3-GST

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pFU-cMVIIA-PE

Plasmid

#24147

Depositor

Ines Ibanez-Tallon

Article

Auer et al Nat Methods. 2010 Feb 7. ():.

Insert

loxP-DsRed-loxP-omega-Conotoxin MVIIA-FLAG-PDGF_R_TM-EGFP (PDGFRB Human, Conus magus, Aequorea victoria)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2010

Availability

Academic Institutions and Nonprofits only

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pFU-iMVIIA-PE

Plasmid

#24146

Depositor

Ines Ibanez-Tallon

Article

Auer et al Nat Methods. 2010 Feb 7. ():.

Insert

omega-Conotoxin MVIIA-FLAG-PDGF_R_TM-EGFP-IRES-TetR-KRAB (PDGFRB Human, Conus magus, Aequorea victoria)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2010

Availability

Academic Institutions and Nonprofits only

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pAGM9121_Cfo-CPO SP

Plasmid

#153500

Purpose

Caldariomyces (Leptoxyphium) fumago Chloroperoxidase Signal Peptide Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

Chloroperoxidase SP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-SAG1-DHFR

Plasmid

#80322

Purpose

Encodes a CRISPR sgRNA that allows for mutation of the surface antigen SAG1 in Toxoplasma gondii and confers resistance to pyrimethamine

Depositor

Sebastian Lourido

Article

Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

Insert

SAG1 sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pTUB1:YFP-AID-3HA, DHFR-TS:HXGPRT

Plasmid

#87260

Purpose

YFP-AID-3HA fusion driven by a minimal TUB1 promoter with an HXGPRT drug selectable marker. The AID CDS is Arabidopsis thaliana auxin-responsive protein IAA17.

Depositor

David Sibley

Article

Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d

Inserts

YFP-AID-3HA
HXGPRT

Use

Toxoplasma expression

Tags

3HA and AID

Expression

Mutation

Promoter

TgDHFR-TS 463 bp and TgTUB1 378 bp

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pMAL_dstβL

Plasmid

#73805

Purpose

Plasmid for selection for insertion and homodimerization

Depositor

Sarel Fleishman

Article

Elazar et al Elife. 2016 Jan 29;5. pii: e12125. doi:

Inserts

CAT Marker
ToxR
beta lactamase
spectomycin resistance
CLS TM domain

Use

Tags

Part Of the dstbl chiemra, Part of the dsTbL chim…

Expression

Bacterial

Mutation

Promoter

NaN and ToxR promotor

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
pDragon-DTA

Plasmid

#155017

Purpose

For Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA, switching to attenuated diphtheria toxin (tox176) in the presence of Cre and (r)tTA activity

Depositor

Alberto Rosello-Diez

Article

Ahmadzadeh et al Development. 2020 May 28;147(10). pii: d

Insert

DTA

Use

Mouse Targeting

Tags

Expression

Mutation

tox176

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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Pet15b-Endosomal Escape Control

Plasmid

#186552

Purpose

Negative Control confirming the need for a CPP peptide in mediating internalization

Depositor

Jean Gariepy

Article

Mohammed et al J Control Release. 2012 Nov 28;164(1):58

Insert

6H-ETA-GFP

Use

Tags

6xHis and EGFP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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Pet15b-Internalization and Endosomal Escape

Plasmid

#186551

Purpose

Internalization and Endosomal Escape to effectively route a protein cargo to the cytosol of cells

Depositor

Jean Gariepy

Article

Mohammed et al J Control Release. 2012 Nov 28;164(1):58

Insert

10R-8His-ETA-EGFP

Use

Tags

8xHis and EGFP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMALp2x-SCO5461

Plasmid

#42507

Depositor

Koji Okamoto

Article

Nakano et al Toxicon. 2012 Dec 2;63C:55-63. doi: 10.1

Insert

guanosine ADP-ribosyltransferase (SCO5461 Streptomyces coelicolor A3(2))

Use

Tags

maltose-binding protein

Expression

Bacterial

Mutation

Promoter

tac

Available since

April 22, 2013

Availability

Academic Institutions and Nonprofits only
pET-20b-T7-Ntx

Plasmid

#58714

Purpose

Expresses T7-Notexin in Escherichia coli

Depositor

Fiorella Tonello

Article

Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14

Insert

Notexin

Use

Tags

T7 peptide tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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ND4-DdCBE- left side TALE

Plasmid

#157844

Purpose

expresses left-side TALE–DddAtox half ND4-DdCBE in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

SOD2 MTS–3x-HA–ND4 left tale–G1397 DddA-C–UGI–SOD2 3'UTR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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ND4-DdCBE-right side TALE

Plasmid

#157843

Purpose

expresses right-side TALE–DddAtox half ND4-DdCBE in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

COX8A MTS–3xFLAG–ND4 right TALE–G1397 DddA-N–UGI–ATP5B 3'UTR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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ND6-DdCBE-left side TALE

Plasmid

#157842

Purpose

expresses left-side TALE–DddAtox half of ND6-DdCBE in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

SOD2 MTS–3x-HA–ND6 left tale–G1397 DddA-N–UGI–SOD2 3'UTR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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