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Showing: 5951 - 6000 of 58032 results

  1. R777-E234 Hs.RASAL1-nostop

    Plasmid
    #70518
    Purpose
    Gateway ORF clone of human RASAL1 [NM_001193521.1] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RASAL1 (RASAL1 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    R331H
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70518-plasmid-map-sequence-id-125967

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  2. pZNF423-donor

    Plasmid
    #113822
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF423 with GFP
    Depositor
    Kevin White
    Insert
    ZNF423 homology arms flanking EGFP-IRES-Neo cassette (ZNF423 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    113822-plasmid-map-sequence-id-230193

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  3. mAmetrine-LaminB1-10

    Plasmid
    #56547
    Purpose
    Localization: Nuclear Envelope, Excitation: 406, Emission: 526
    Depositor
    Michael Davidson
    Insert
    LaminB1 (LMNB1 Human)
    Use
    Tags
    mAmetrine
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 13, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  4. p-dCas9-SET7-Hygro

    Plasmid
    #104407
    Purpose
    transient expression of dCas9-SET7 fusion protein
    Depositor
    Stephan Beck
    Insert
    SET7 (SETD7 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104407-plasmid-map-sequence-id-204449

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  5. R777-E088 Hs.FGFR3-nostop

    Plasmid
    #70372
    Purpose
    Gateway ORF clone of human FGFR3 [NM_000142.4] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    FGFR3 (FGFR3 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70372-plasmid-map-sequence-id-125821

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  6. UHRF1

    Plasmid
    #25359
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ubh12.672.793 (UHRF1 Human)
    Use
    Tags
    His with thrombin cleavage site
    Expression
    Bacterial
    Mutation
    amino acid residues 672-793.
    Promoter
    Available since
    Dec. 21, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  7. R777-E075 Hs.EXOC5

    Plasmid
    #70359
    Purpose
    Gateway ORF clone of human EXOC5 [NM_006544.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    EXOC5 (EXOC5 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70359-plasmid-map-sequence-id-125808

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  8. R777-E077 Hs.EXOC6

    Plasmid
    #70361
    Purpose
    Gateway ORF clone of human EXOC6 [NM_019053.4] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    EXOC6 (EXOC6 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70361-plasmid-map-sequence-id-125810

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  9. R777-E079 Hs.EXOC7

    Plasmid
    #70363
    Purpose
    Gateway ORF clone of human EXOC7 [NM_001013839.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    EXOC7 (EXOC7 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70363-plasmid-map-sequence-id-125812

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  10. pHBS1051 TDP43 RRM-GFP F-Y

    Plasmid
    #107802
    Purpose
    To test the effect of sequence on TDP43 droplet properties
    Depositor
    Rajat Rohatgi
    Insert
    TARDBP mutant (TARDBP Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    All phenylalanine to tyrosine in LLPS reporter
    Promoter
    Available since
    April 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    107802-plasmid-map-sequence-id-208177

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  11. R777-E316 Hs.SHOC2-nostop

    Plasmid
    #70600
    Purpose
    Gateway ORF clone of human SHOC2 [NM_007373.3] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    SHOC2 (SHOC2 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70600-plasmid-map-sequence-id-126049

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  12. mTurquoise-ALK1-C-18

    Plasmid
    #55521
    Purpose
    Localization: Membrane Receptor, Excitation: 434, Emission: 474
    Depositor
    Michael Davidson
    Insert
    ALK1 (ACVRL1 Human)
    Use
    Tags
    mTurquoise
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    55521-plasmid-map-sequence-id-209501

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  13. MLH1

    Plasmid
    #28120
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    MLH1:JMC01L-F09:C205690 (MLH1 Human)
    Use
    Tags
    His tag with TEV site
    Expression
    Bacterial
    Mutation
    contains amino acid residues 1-347.
    Promoter
    Available since
    March 21, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. SOCS6

    Plasmid
    #38871
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SOCS6 (SOCS6 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    V368D, R463G
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  15. YPet-LaminB1-10

    Plasmid
    #56635
    Purpose
    Localization: Nuclear Envelope, Excitation: 513, Emission: 527
    Depositor
    Michael Davidson
    Insert
    LaminB1 (LMNB1 Human)
    Use
    Tags
    YPet
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 19, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  16. USP37

    Plasmid
    #36898
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    USP37 (USP37 Human)
    Use
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    contains amino acid residues 4-125 only
    Promoter
    Available since
    June 21, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  17. mCherry-ATG12-N-18

    Plasmid
    #54986
    Purpose
    Localization: Membrane Receptor, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    ATG12 (ATG12 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  18. tdEos-Rab11a-7

    Plasmid
    #57664
    Purpose
    Localization: Ras GTPase, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson
    Insert
    Rab11a (RAB11A Human)
    Use
    Tags
    tdEos
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 13, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    57664-plasmid-map-sequence-id-209006

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  19. mKO2-ER-5

    Plasmid
    #57872
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 551, Emission: 565
    Depositor
    Michael Davidson
    Insert
    ER (CALR Human)
    Use
    Tags
    mKO2
    Expression
    Mammalian
    Mutation
    ER Signal Peptide
    Promoter
    CMV
    Available since
    Oct. 24, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. R777-E178 Hs.PIK3R4-nostop

    Plasmid
    #70462
    Purpose
    Gateway ORF clone of human PIK3R4 [NM_014602.2] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    PIK3R4 (PIK3R4 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70462-plasmid-map-sequence-id-125911

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  21. PX458_HHEX_1

    Plasmid
    #72354
    Purpose
    Encodes gRNA for 3' target of human HHEX along with Cas9 with 2A GFP
    Depositor
    Eric Mendenhall, Richard M. Myers
    Insert
    HHEX (HHEX Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    72354-plasmid-map-sequence-id-134606

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  22. tdEos-ER-5

    Plasmid
    #57611
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson
    Insert
    ER (CALR Human)
    Use
    Tags
    tdEos
    Expression
    Mammalian
    Mutation
    ER Signal Peptide
    Promoter
    CMV
    Available since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  23. mTurquoise-Fibrillarin-7

    Plasmid
    #55552
    Purpose
    Localization: Nucleoli, Excitation: 434, Emission: 434
    Depositor
    Michael Davidson
    Insert
    Fibrillarin (FBL Human)
    Use
    Tags
    mTurquoise
    Expression
    Mammalian
    Mutation
    R36Q, S66F
    Promoter
    CMV
    Available since
    Oct. 7, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    55552-plasmid-map-sequence-id-328411

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  24. pHBS961 TDP43 RRM-GFP G295S

    Plasmid
    #107820
    Purpose
    To test the effect of sequence on TDP43 droplet properties
    Depositor
    Rajat Rohatgi
    Insert
    TARDBP mutant (TARDBP Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    G295S mutation in LLPS reporter
    Promoter
    Available since
    April 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    107820-plasmid-map-sequence-id-207933

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  25. mVenus-Fibrillarin-7

    Plasmid
    #56613
    Purpose
    Localization: Nucleoli, Excitation: 515, Emission: 528
    Depositor
    Michael Davidson
    Insert
    Fibrillarin (FBL Human)
    Use
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    R36Q, S66F
    Promoter
    CMV
    Available since
    Dec. 23, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  26. mWasabi-EB3-7

    Plasmid
    #56499
    Purpose
    Localization: MT End Binding Protein, Excitation: 493, Emission: 509
    Depositor
    Michael Davidson
    Insert
    EB3 (MAPRE3 Human)
    Use
    Tags
    mWasabi
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 6, 2015
    Availability
    Academic Institutions and Nonprofits only

  27. Dronpa-Actin-C-18

    Plasmid
    #57259
    Purpose
    Localization: Actin, Excitation: 503, Emission: 518
    Depositor
    Michael Davidson
    Insert
    Actin (ACTB Human)
    Use
    Tags
    Dronpa
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 13, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  28. TNKS2A

    Plasmid
    #42454
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    TNKS2A (TNKS2 Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  29. Dronpa-Calnexin-N-14

    Plasmid
    #57264
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 503, Emission: 518
    Depositor
    Michael Davidson
    Insert
    NM_001746.3 (CANX Human)
    Use
    Tags
    Dronpa
    Expression
    Mammalian
    Mutation
    I474N in Calnexin
    Promoter
    CMV
    Available since
    Jan. 16, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  30. pHH0103 APBB3 WW domain

    Plasmid
    #104262
    Purpose
    Bacterial expression of WW domain from APBB3
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    APBB3 WW domain (APBB3 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104262-plasmid-map-sequence-id-203722

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  31. pHH0103 APBB2 WW domain

    Plasmid
    #104257
    Purpose
    Bacterial expression of WW domain from APBB2
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    APBB2 WW domain (APBB2 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    104257-plasmid-map-sequence-id-201510

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  32. mAzurite-Cx32-7

    Plasmid
    #55230
    Purpose
    Localization: Gap Junctions, Excitation: 384, Emission: 450
    Depositor
    Michael Davidson
    Insert
    Cx32 (GJB1 Human)
    Use
    Tags
    mAzurite
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  33. pZNF331.1.0-gDNA

    Plasmid
    #112461
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF331
    Depositor
    Kevin White
    Insert
    ZNF331 (ZNF331 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112461-plasmid-map-sequence-id-230479

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  34. mPlum-Actin-12

    Plasmid
    #55948
    Purpose
    Localization: Actin, Excitation: 590, Emission: 649
    Depositor
    Michael Davidson
    Insert
    Actin (ACTB Human)
    Use
    Tags
    mPlum
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  35. pZBTB11-donor

    Plasmid
    #112348
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZBTB11 with GFP
    Depositor
    Kevin White
    Insert
    ZBTB11 homology arms flanking EGFP-IRES-Neo cassette (ZBTB11 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:3:101651181:A>T
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112348-plasmid-map-sequence-id-229661

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  36. pVEZF1.1.0-gDNA

    Plasmid
    #112437
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor VEZF1
    Depositor
    Kevin White
    Insert
    VEZF1 (VEZF1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    112437-plasmid-map-sequence-id-230392

    Enlarge

  37. R777-E030 Hs.CNKSR2-nostop

    Plasmid
    #70314
    Purpose
    Gateway ORF clone of human CNKSR2 [NM_001168647.1] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    CNKSR2 (CNKSR2 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70314-plasmid-map-sequence-id-125763

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  38. NT5C3A

    Plasmid
    #42355
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    NT5C3A (NT5C3A Human)
    Use
    Tags
    His6-Thrombin
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  39. R777-E074 Hs.EXOC4-nostop

    Plasmid
    #70358
    Purpose
    Gateway ORF clone of human EXOC4 [NM_021807.3] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    EXOC4 (EXOC4 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70358-plasmid-map-sequence-id-125807

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  40. R777-E068 Hs.EXOC1-nostop

    Plasmid
    #70352
    Purpose
    Gateway ORF clone of human EXOC1 [NM_001024924.1] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    EXOC1 (EXOC1 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70352-plasmid-map-sequence-id-125801

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  41. R777-E072 Hs.EXOC3-nostop

    Plasmid
    #70356
    Purpose
    Gateway ORF clone of human EXOC3 [NM_007277.4] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    EXOC3 (EXOC3 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70356-plasmid-map-sequence-id-125805

    Enlarge

  42. tdTomato-Actin-C-18

    Plasmid
    #58069
    Purpose
    Localization: Actin, Excitation: 554, Emission: 581
    Depositor
    Michael Davidson
    Insert
    Actin (ACTB Human)
    Use
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    58069-plasmid-map-sequence-id-306363

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  43. R777-E360 Hs.YAP1-nostop

    Plasmid
    #70644
    Purpose
    Gateway ORF clone of human YAP1 [NM_001130145.2] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    YAP1 (YAP1 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70644-plasmid-map-sequence-id-126093

    Enlarge

  44. mApple-GFAP-N-18

    Plasmid
    #54906
    Purpose
    Localization: Intermediate Filaments, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    GFAP (GFAP Human)
    Use
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  45. OTU04

    Plasmid
    #28157
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    OTU domain containing 7A (OTUD7A Human)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    UBA-like domain only
    Promoter
    Available since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  46. mCherry-ATG14-N-18

    Plasmid
    #54990
    Purpose
    Localization: Membrane Receptor, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    ATG14 (ATG14 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only

  47. R777-E156 Hs.PDGFRA-nostop

    Plasmid
    #70440
    Purpose
    Gateway ORF clone of human PDGFRA [NM_006206.4] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    PDGFRA (PDGFRA Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70440-plasmid-map-sequence-id-125889

    Enlarge

  48. mCherry-Cx32-7

    Plasmid
    #55022
    Purpose
    Localization: Gap Junctions, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Cx32 (GJB1 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only

  49. p14-9VNA5

    Plasmid
    #50931
    Purpose
    Expresses human SRP14A5 and SRP9 proteins as a fusion protein in mammalian cells.
    Depositor
    Katharina Strub
    Insert
    SRP14 and SRP9 (SRP14, SRP9 Human)
    Use
    Tags
    VN tag and flag tag
    Expression
    Mammalian
    Mutation
    Amino acids 96-100 of SRP14 changed to alanine
    Promoter
    CMV
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  50. CSNK1G2

    Plasmid
    #39150
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CSNK1G2 (CSNK1G2 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
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