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Plasmid

#62449

Purpose

MXS_chaining vector with 6 cassettes

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Inserts

CMV::GTS-Citrine-bGHpA
CMV::Lyn-Ceruleanx3-bGHpA
CMV::NLS-TagBFPx3-bGHpA
CMV::MTS-AzamiGreen-bGHpA
CMV::iRFP670-SKL-bGHpA
CMV::ETS-mCherry-KDEL-bGHpA

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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Ki67-mNeonGreen-IRESpuro2

Plasmid

#183737

Purpose

Expresses Ki67-mNeonGreen

Depositor

Daniel Gerlich

Article

Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

Insert

MKI67 (MKI67 Human)

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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plenti-UBC-gate-3xHA-pGK-PUR

Plasmid

#107393

Purpose

Lentiviral gateway destination vector

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Type

Empty backbone

Use

Lentiviral; Gateway destination

Tags

3xHA

Expression

Mammalian

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-8xHis-Ub

Plasmid

#107392

Purpose

Mammalian expression 8xHis-Ub

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

8 tandem repeats of 6xHis-Ub (See comments below)

Tags

None

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1 - TRC mTagBFP2

Plasmid

#191566

Purpose

Expresses an shRNA and mTagBFP2

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6 for shRNA

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-sRGN3.1-BlastR

Plasmid

#220964

Purpose

Expresses human codon-optimized sRGN3.1

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

sRGN3.1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-NLS-copGFP-EF1-BlastiS

Plasmid

#132772

Purpose

Spills into the cytoplasm during nuclear envelope rupture.

Depositor

Jan Lammerding

Article

Denais et al Science. 2016 Apr 15;352(6283):353-8. do

Insert

NLS-copGFP

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pM201

Plasmid

#173179

Purpose

dCas9-24xGCN4

Depositor

Hajin Kim

Article

Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

Insert

dCas9-24xGCN4

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pgRNA-modified

Plasmid

#84477

Purpose

guide RNA expression lentiviral construct

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

guide RNA

Use

Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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TDP-43 mRuby

Plasmid

#216225

Purpose

TDP-43 with a C-terminal mRuby tag for expression in mammalian cells.

Depositor

Jennifer Lee

Article

Shadish et al Biophys Chem. 2024 Jan 23;307:107191. do

Insert

TDP-43 (TARDBP Human)

Tags

mRuby

Expression

Mammalian

Promoter

CMV

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mCherry-CyrCR1

Plasmid

#128768

Purpose

Expresses mCherry in mammalian cells. ~200 nt of Cyrano inserted in 3'UTR with miR-7 site replaced by miR-17 site.

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

Cyrano conserved region (CR1) with intact miR-7 site (OIP5-AS1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pM355

Plasmid

#173175

Purpose

(Empty Backbone) Expresses gRNA-12xMBS from hU6 promoter

Depositor

Hajin Kim

Article

Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

Insert

gRNA-12xMBS backbone

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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mNG2(1-10)_tdTomato

Plasmid

#184169

Purpose

Lentiviral vector for expression and selection of the mNG2(1-10) split fluorescent protein.

Depositor

Ophir Shalem

Article

Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

Inserts

mNG2(1-10)
tdTomato

Use

Lentiviral

Expression

Mammalian

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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plenti-CAG-gate-FLAG-IRES-GFP

Plasmid

#107398

Purpose

Lentiviral gateway destination vector

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Type

Empty backbone

Use

Lentiviral; Gateway destination

Tags

FLAG

Expression

Mammalian

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD-Lime

Plasmid

#191255

Purpose

Bacterial expression plasmids for pH sensitive fluorescent protein Lime

Depositor

Robert Campbell

Article

Shen et al ACS Sens. 2023 Aug 25;8(8):3014-3022. do

Insert

Lime

Tags

His tag

Expression

Bacterial

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCS Omomyc

Plasmid

#113164

Purpose

Omomyc expression in mammalian cells

Depositor

Sergio Nasi

Article

Soucek et al Oncogene. 1998 Nov 12;17(19):2463-72. do

Insert

Omomyc (MYC Human)

Expression

Mammalian

Mutation

E57T, E64I, R70Q, R71N

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pET21a-GlyRS-His

Plasmid

#124110

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Glycyl-tRNA ligase (glyS, glyQ Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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Cyto-V5-APEX2

Plasmid

#170572

Purpose

Expresses APEX2 in cytosol

Depositor

James Olzmann

Article

Bersuker et al Dev Cell. 2018 Jan 8;44(1):97-112.e7. do

Insert

APEX2

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pQE30-His-PheRS

Plasmid

#124116

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Phenylalanyl-tRNA ligase (pheS, pheT Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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pSK-mU6-Cj-sgRNA-SV40-puro

Plasmid

#192128

Purpose

Expresses Cj-SgRNA scaffold cloned with BbsI and puromycin resistance gene

Depositor

Yongming Wang

Article

Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

Insert

Cj-SgRNA scaffold

Expression

Mammalian

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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