We narrowed to 6,827 results for: SIM

1. SPOON/ pcDNA3

   Plasmid

   #124840

   Purpose

   mammalian expression of SPOON, a spontaneously blinking green fluorescent protein

   Depositor

   Takeharu Nagai

   Article

   Arai et al ACS Chem Biol. 2018 Aug 17;13(8):1938-19

   Insert

   SPOON
   

   Use

   Tags

   No tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSSM16: pCMV-HA-NLS-SpuFz1

   Plasmid

   #205271

   Purpose

   Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1

   Depositor

   Feng Zhang

   Article

   Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

   Insert

   SpuFz1
   

   Use

   Tags

   HA, NLS(Makkerh)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJL059

   Plasmid

   #177210

   Purpose

   Bicistronic vector with GFP reporter

   Depositor

   Brittany Adamson

   Article

   Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMAL358

   Plasmid

   #169147

   Purpose

   Encodes OptoQ-AMP1

   Depositor

   Jose Avalos

   Article

   Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

   Insert

   VP16-EL222
   

   Use

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJAT56

   Plasmid

   #204300

   Purpose

   Express phiC31 under control of D. melanoagaster vasa promoter, with Vasa 3’UTR.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   3XP3-EGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   3XP3

   Available since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. LeGO-iG2-wPRE-pA

   Plasmid

   #60489

   Purpose

   eGFP-expressing lentiviral vector with IRES and pA-signal; suitable for use as NRTLV (non-reverse transcribable lentiviral vector)

   Depositor

   Boris Fehse

   Article

   Mock et al Sci Rep. 2014 Sep 18;4:6409. doi: 10.103

   Insert

   IRES-eGFP; wPRE; BGH-pA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMAL513

   Plasmid

   #169153

   Purpose

   Encodes OptoQ-INVRT1

   Depositor

   Jose Avalos

   Article

   Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

   Insert

   VP16-EL222
   

   Use

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEN_TTmiRc2

   Plasmid

   #25752

   Purpose

   Entry vector for cloning miR30-based shRNA driven by TRE-tight promoter.

   Depositor

   Iain Fraser

   Article

   Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

   Type

   Empty backbone

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJAT21

   Plasmid

   #204309

   Purpose

   For generating targeting inversions. N-terminal split GFP, expressed in abdomen and mouthparts

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   ie1-GFPn
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   ie1

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJAT25

    Plasmid

    #204310

    Purpose

    For generating targeting inversions. C-terminal split GFP, expressed in abdomen and mouthparts.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    ie1-GFPc
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ie1

    Available since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmax-3xFLAG_3xNLS_dPspCas13b(AAAA)_2xNLS-Clover

    Plasmid

    #196829

    Purpose

    Express dpdpCas13b with AAAA mutation and Clover

    Depositor

    Albert Cheng

    Article

    Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

    Insert

    dpspCas13b(AAAA)
    

    Use

    Tags

    3XFLAG and NES

    Expression

    Mammalian

    Mutation

    K367A, D369A and K370A

    Promoter

    Available since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. 4 gRNA concatemer

    Plasmid

    #84881

    Purpose

    Empty concatmer vector in which 4 gRNAs can be inserted

    Depositor

    Bon-Kyoung Koo

    Article

    Andersson-Rolf et al Dev Biol. 2016 Oct 27. pii: S0012-1606(1

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. PlayBack-CMV-Kpn1

    Plasmid

    #203302

    Purpose

    For expression of eukaryotic genes of interest with CMV promoter with Kpn1 as a PlayBack Restriction Enzyme

    Depositor

    Aleksandar Necakov

    Article

    Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. apt-CBE-3'end

    Plasmid

    #219939

    Purpose

    Mammalian expression of apt-CBE-3'end protein (MCP_32aa-linker_evoAPOBEC1) with P2A-EGFP marker

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    MCP_32-aa_evo-rA1_2xUGI
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. apt-ABE8e

    Plasmid

    #219937

    Purpose

    Mammalian expression of apt-ABE8e protein (Com_93aa-linker_TadA8e) with P2A-EGFP marker

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    Com_93-aa_TadA8e
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMJ106: pRS426-His-MBP-NlovFz2_Native

    Plasmid

    #205253

    Purpose

    S. cerevisiae expression vector for NlovFz2 native guide

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    NlovFz2
    

    Use

    Tags

    10xHis, MBP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMJ45: pRS426-His-MBP-SpuFz1_Native

    Plasmid

    #205252

    Purpose

    S. cerevisiae expression vector for SpuFz1 native guide

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1
    

    Use

    Tags

    10xHis, MBP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMJ332: pU6-GtFz1_omegaRNA-entry

    Plasmid

    #205265

    Purpose

    Human expression vector for GtFz1 omegaRNA (BbsI guide entry site)

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    GtFz1 omegaRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 3TERM_35S

    Plasmid

    #136114

    Purpose

    3TERM after CDS/ CDS12-CTAG

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    3TERM_35S (CaMV)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pMJ123: pRS426-His-MBP-SpuFz1_PSP1

    Plasmid

    #205256

    Purpose

    S. cerevisiae expression vector for SpuFz1 reprogrammed guide (targeting PSP1)

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1
    

    Use

    Tags

    10xHis, MBP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge