We narrowed to 5,111 results for: codon optimized

1. pSW002-PpsbA_mTurquoise2

   Plasmid

   #108237

   Purpose

   Broad host bacterial expression vector with constitutive PsbA promoter. Expresses mTurquoise2; codon optimized for P. fluorescens.

   Depositor

   Rosemarie Wilton

   Article

   Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

   Insert

   PsbA promoter - mTurquoise2
   

   Expression

   Bacterial

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSQT1601

   Plasmid

   #53369

   Purpose

   human codon optimized Csy4 and FokI-dCas9 expression plasmid

   Depositor

   Keith Joung

   Article

   Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

   Insert

   hCsy4-T2A-NLS-hFokI-dCas9-NLS
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. lentiCas9n(D10A)-Blast

   Plasmid

   #63593

   Purpose

   Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Inserts

   Cas9
   Blasticidin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A, nickase mutant

   Promoter

   EFS-NS

   Available Since

   April 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pscALPS- SIV-MAC251 vpx

   Plasmid

   #115810

   Purpose

   Encodes codon optimized 3xFLAG-VpxMAC251 and puromycin resistance protein

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   SIVMAC251 Vpx
   

   Use

   Lentiviral

   Tags

   3x-FLAG

   Promoter

   SFFV

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIntegrase_8

   Plasmid

   #60579

   Purpose

   plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Integrase_8
   

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pBAD

   Available Since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRCC-K

   Plasmid

   #81191

   Purpose

   Expression of Cas9 and gRNA cassette in S. cerevisiae; Kan Resistance

   Depositor

   Eckhard Boles

   Article

   Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

   Inserts

   Cas9
   empty gRNA cassette
   

   Use

   CRISPR

   Tags

   NTS

   Expression

   Yeast

   Mutation

   Please see depositor comments below. and WT - cod…

   Promoter

   ROX3 and SNP52p

   Available Since

   Sept. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPRyl

   Plasmid

   #70007

   Purpose

   CRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertion

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al ACS Synth Biol. 2015 Dec 29.

   Inserts

   Codon optimized Cas9 from S. pyogenes
   sgRNA expression cassette
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Promoter

   SCR1'-tRNA and UAS1B8-TEF(136)

   Available Since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPP441

   Plasmid

   #158801

   Purpose

   Plasmid containing Homo sapiens codon optimized CasΦ-2 and a SapI-GG stuffer spacer for editing in human cells.

   Depositor

   Jennifer Doudna

   Article

   Pausch et al Science. 2020 Jul 17;369(6501):333-337.

   Insert

   CasPhi-2
   

   Tags

   2xSV40NLS-2xFLAG

   Expression

   Mammalian

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMK364 (CMV-OsTIR1-loxP-PURO-loxP)

   Plasmid

   #121184

   Purpose

   OsTIR1 introduction

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

   Insert

   CMV-OsTIR1-loxP-PURO-loxP
   

   Use

   CRISPR

   Mutation

   OsTIR1 codon optimized for human cells

   Available Since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZyxin-mMaple3

    Plasmid

    #101151

    Purpose

    Fluorescent protein for superresolution imaging and a focal adhesion protein for binding substrate

    Depositor

    Xiaowei Zhuang

    Article

    Wang et al Proc Natl Acad Sci U S A. 2014 Jun 10;11

    Insert

    human codon optimized mMaple3 fused to zyxin
    

    Expression

    Mammalian

    Promoter

    cmv

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-N1-human lyn–GFP

    Plasmid

    #35958

    Purpose

    Expresses human lyn protein codon optimized for zebrafish expression.

    Depositor

    Anna Huttenlocher

    Article

    Yoo et al Nature. 2011 Nov 20;480(7375):109-12. do

    Insert

    LYN (LYN Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    cmv

    Available Since

    April 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pETM6-mCherry

    Plasmid

    #66534

    Purpose

    Expresses mCherry under the control of the Concensus T7 Promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Codon Optimized for E. coli

    Promoter

    Consensus T7

    Available Since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. Punc-4c.TIR1

    Plasmid

    #194053

    Purpose

    TIR1 expression vector under the unc-4c promoter expressed in the DA motor neurons in C. elegans

    Depositor

    Kota Mizumoto

    Article

    Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

    Insert

    TIR1 (TIR1 Mustard Weed)
    

    Expression

    Worm

    Mutation

    codon-optimized for expression in C. elegans; Two…

    Promoter

    unc-4c

    Available Since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSL4393 (pClpX_Eco)

    Plasmid

    #200907

    Purpose

    Expresses human codon-optimized E. coli ClpX from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    EcoClpX
    

    Tags

    NLS

    Expression

    Mammalian

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSL3713 (pTnsC_Pse)

    Plasmid

    #200900

    Purpose

    Expresses human codon-optimized Pseudoalteromonas TnsC from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    PseTnsC
    

    Tags

    3xNLS

    Expression

    Mammalian

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMSSM110: pCMV-HA-NLS-SpuFz1-v2

    Plasmid

    #205277

    Purpose

    Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-v2

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1
    

    Tags

    HA, NLS(Makkerh)

    Expression

    Mammalian

    Mutation

    C310R/D487K/T513K

    Available Since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. 3xFLAG-dCas9/pMXs-neo

    Plasmid

    #51260

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR and Retroviral

    Tags

    3xFLAG tag and NLS (nuclear localization signal)

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    LTR

    Available Since

    Feb. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pME-Cas9

    Plasmid

    #63154

    Purpose

    Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS

    Depositor

    Leonard Zon

    Article

    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

    Insert

    Cas9
    

    Use

    CRISPR; Tol2 middle entry vector for gateway

    Available Since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCRISPRa_VPR_yl

    Plasmid

    #107677

    Purpose

    CRISPR-dCas9-VPR vector for Yarrowia lipolytica, expressing dCas9-VPR and AvrII site for sgRNA insertion

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al Biotechnol J. 2018 May 5:e1700584. doi:

    Inserts

    Codon optimized dCas9-VPR
    sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Promoter

    SCR1'-tRNA and UAS1B8-TEF(136)

    Available Since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAGGS-Bxb1o_nlsFlag

    Plasmid

    #119901

    Purpose

    Expresses Bxb1 integrase in mammalian cells

    Depositor

    Orion Weiner

    Article

    Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

    Insert

    Mammalian codon optimized Bxb1 integrase
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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