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We narrowed to 5,133 results for: codon optimized

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Showing: 2381 - 2400 of 5133 results

  3. pRCC-K

    Plasmid
    #81191
    Purpose
    Expression of Cas9 and gRNA cassette in S. cerevisiae; Kan Resistance
    Depositor
    Eckhard Boles
    Article
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please see depositor comments below. and WT - cod…
    Promoter
    ROX3 and SNP52p
    Available Since
    Sept. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    81191-plasmid-map-sequence-id-154417

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  4. pCgCas9_recT

    Plasmid
    #153337
    Purpose
    Double-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; pBL1ts oriVC. glutamicum; pSC101 oriVE. coli PlacM-SpCas9 Streptomyces coelicolor codon-optimized, Peftu-RecT; Kanr
    Depositor
    Sheng Yang
    Article
    Zhang et al ACS Synth Biol. 2020 Jul 17;9(7):1897-19
    Insert
    Cas9
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    153337-plasmid-map-sequence-id-305977

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  5. pCRISPRi_Mxi1_yl

    Plasmid
    #91248
    Purpose
    CRISPR-dCas9-Mxi1 vector for Yarrowia lipolytica, expressing dCas9-Mxi1 and AvrII site for sgRNA insertion
    Depositor
    Ian Wheeldon
    Article
    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.
    Inserts
    Codon optimized dCas9-Mxi1
    sgRNA expression cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Yeast
    Promoter
    SCR1'-tRNA and UAS1B8-TEF(136)
    Available Since
    Aug. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    91248-plasmid-map-sequence-id-192174

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  6. pRBC-sfGFP-134/150TAG

    Plasmid
    #174077
    Purpose
    Plasmid with p15a origin of replication expressing codon optimized superfolder GFP-134/150TAG with C-terminal His6 tag, under T7 promoter
    Depositor
    Ryan Mehl
    Article
    Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15
    Insert
    Superfolder GFP
    Tags
    His-6
    Expression
    Bacterial
    Mutation
    D134TAG + N150TAG
    Promoter
    T7
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    174077-plasmid-map-sequence-id-345699

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  16. pCB-2

    Plasmid
    #210388
    Purpose
    All-in-one cumate-based inducible CRISPRi plasmid for use in Streptomyces
    Depositor
    Gilles van Wezel
    Article
    Bai et al ACS Synth Biol. 2023 Oct 6. doi: 10.1021
    Inserts
    dCas9
    CymR
    sgRNA cassette
    Use
    CRISPR; Integrative vector for use in streptomyces
    Mutation
    BsaI-flanked RFP cassette inserted in place of sp…
    Promoter
    SP11 and SP30
    Available Since
    Jan. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    210388-plasmid-map-sequence-id-418474

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  20. 3xFLAG-dCas9/pMXs-neo

    Plasmid
    #51260
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available Since
    Feb. 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    51260-plasmid-map-sequence-id-77871

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Showing: 2381 - 2400 of 5133 results