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pTaCas9-dead_5

Plasmid

#91174

Purpose

Cas9 only expression, Cas9 Type: TaCas9_D10A+H840A (dead), Plant Selection: PvUbi2:hpt II

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TaCas9_D10A+H840A (dead)

Use

CRISPR

Expression

Plant

Mutation

D10A, H840A

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pZS1[GalS-T]

Plasmid

#60753

Purpose

Contains PIq driving expression GalS-T, the Fucose inducible chimera with the TAN DBD.

Depositor

Matthew Bennett

Publication

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

GalS-T
GalS-T

Use

Synthetic Biology

Expression

Bacterial

Mutation

Chimeric LacI/GalR repressor with the TAN DBD and…

Available since

July 10, 2015

Availability

Academic Institutions and Nonprofits only

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pZS1[GalS-L,LacI-L]

Plasmid

#60759

Purpose

Contains PIq driving expression GalS-L, and PI driving expression of LacI-L.

Depositor

Matthew Bennett

Publication

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

GalS-L
wt LacI

Use

Synthetic Biology

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. LacI DBD with GalS L…

Available since

July 10, 2015

Availability

Academic Institutions and Nonprofits only

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pZS1[GalS-L,RbsR-L]

Plasmid

#60763

Purpose

Contains PIq driving expression GalS-L, and PIq driving expression of RbsR-L.

Depositor

Matthew Bennett

Publication

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

GalS-L
RbsR-L

Use

Synthetic Biology

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. LacI DBD with GalS L…

Available since

July 10, 2015

Availability

Academic Institutions and Nonprofits only

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pZS1[GalS-L,FruR-L]

Plasmid

#60764

Purpose

Contains PIq driving expression GalS-L, and PIq driving expression of FruR-L.

Depositor

Matthew Bennett

Publication

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

GalS-L
FruR-L

Use

Synthetic Biology

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. LacI DBD with FruR L…

Available since

July 10, 2015

Availability

Academic Institutions and Nonprofits only

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pZS1[GalS-L,TreR-L]

Plasmid

#60765

Purpose

Contains PIq driving expression GalS-L, and PI driving expression of TreR-L.

Depositor

Matthew Bennett

Publication

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

GalS-L
TreR-L

Use

Synthetic Biology

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. LacI DBD with GalS L…

Available since

July 10, 2015

Availability

Academic Institutions and Nonprofits only

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pzs1[GalS-L]

Plasmid

#60743

Purpose

Contains PIq driving expression of GalS-R, the Fucose inducible chimera with the wt Lac DBD.

Depositor

Matthew Bennett

Publication

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Insert

GalS-L

Use

Synthetic Biology

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. LacI DBD with GalS L…

Available since

July 10, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pZS1[FruR-L,RbsR-L]

Plasmid

#60761

Purpose

Contains PIq driving expression TreR-L, and PIq driving expression of RbsR-L

Depositor

Matthew Bennett

Publication

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

FruR-L
RbsR-L

Use

Synthetic Biology

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. LacI DBD with FruR L…

Available since

July 10, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pZM3[I:FruR-L,I:RbsR-L]

Plasmid

#60777

Purpose

Contains PI driving expression FruR-L, and PI driving expression of RbsR-L.

Depositor

Matthew Bennett

Publication

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

FruR-L
RbsR-L

Mutation

LacI/GalR repressor chimera. LacI DBD with FruR L…

Available since

July 10, 2015

Availability

Academic Institutions and Nonprofits only

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pZS1[Iq:LacI-T]

Plasmid

#60756

Purpose

Contains PIq driving expression LacI-T, the Lactose inducible chimera with the TAN DBD.

Depositor

Matthew Bennett

Publication

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Insert

LacI-T

Use

Synthetic Biology

Expression

Bacterial

Mutation

LacI without the tetramerization domain and the T…

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pZS1[RbsR-L,TreR-L]

Plasmid

#60766

Purpose

Contains PIq driving expression RbsR-L, and PI driving expression of TreR-L.

Depositor

Matthew Bennett

Publication

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

RbsR-L
TreR-L

Use

Synthetic Biology

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. LacI DBD with RbsR L…

Available since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pZS1[TreR-L,LacI-L]

Plasmid

#60760

Purpose

Contains PI driving expression TreR-L, and PI driving expression of LacI-L

Depositor

Matthew Bennett

Publication

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

TreR-L
Dimeric LacI

Use

Synthetic Biology

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. LacI DBD with TreR L…

Available since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pZS1[RbsR-L,LacI-L]

Plasmid

#60757

Purpose

Contains PIq driving expression RbsR-L, and PI driving expression of LacI-L.

Depositor

Matthew Bennett

Publication

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

RbsR-L
Dimeric LacI

Use

Synthetic Biology

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. LacI DBD with RbsR L…

Available since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pZS1[RbsR-T]

Plasmid

#60751

Purpose

Contains PIq driving expression RbsR-T, the Ribose inducible chimera with the TAN DBD.

Depositor

Matthew Bennett

Publication

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Insert

RbsR-T

Use

Synthetic Biology

Expression

Bacterial

Mutation

Chimeric LacI/GalR repressor with the TAN DBD and…

Available since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pZS1[RbsR-L]

Plasmid

#60741

Purpose

Contains PIq driving expression RbsR-L, the Ribose inducible chimera with the wt Lac DBD.

Depositor

Matthew Bennett

Publication

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Insert

RbsR-L

Use

Synthetic Biology

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. LacI DBD with RbsR L…

Available since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pWal10-roe-sfGFP

Plasmid

#240239

Purpose

Gateway destination plasmid to generate C-terminal sfGFP fusions under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

Depositor

Justin Bosch, Norbert Perrimon

Publication

Bosch et al Nat Commun. 2026 Apr 20. doi: 10.1038/s4

Type

Empty backbone

Use

Gateway Cloning

Expression

Insect

Available since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR_CG6867_nostop

Plasmid

#240238

Purpose

Gateway entry clone with CG6867 without stop codon

Depositor

Justin Bosch, Norbert Perrimon

Publication

Bosch et al Nat Commun. 2026 Apr 20. doi: 10.1038/s4

Insert

CG6867 (CG6867 Fly)

Use

Gateway shuttle vector

Available since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pC1_oROS-HT_LF(C199S)

Plasmid

#216412

Purpose

Expresses the loss-of-function mutation C199S of the genetically encoded, chemigenetic hydrogen peroxide sensor oROS-HT in mamalian cells.

Depositor

Andre Berndt

Publication

Lee et al bioRxiv 2024.02.06.579232

Insert

oROS-HT_LF(C199S)

Expression

Mammalian

Promoter

CMV

Available since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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pJK_ToeRepG2_N64_trigger

Plasmid

#132746

Purpose

T7 RNAP-driven expression of second-generation toehold repressor index 64 trigger RNA

Depositor

Alexander Green, Peng Yin

Publication

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

ToeRepG2_N64_trigger

Expression

Bacterial

Available since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAG_PluxB_ToeRep_N01_trigger

Plasmid

#132736

Purpose

Inducible expression of first-generation toehold repressor index 1 trigger RNA via the PluxB promoter

Depositor

Alexander Green, Peng Yin

Publication

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

PluxB_ToeRep_N01_trigger

Expression

Bacterial

Available since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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