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Showing: 4801 - 4840 of 40591 results

  1. pQCXIP-SHMY-PAUF

    Plasmid
    #182645
    Purpose
    Mammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), one tyrosine sulfation (TS) motif (Y), myc (M), His6 (H) and PAUF sequence
    Depositor
    Martin Spiess
    Article
    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii
    Insert
    Pancreatic adenocarcinoma upregulated factor (PAUF)
    Use
    Retroviral
    Tags
    His6-tag, N-terminal ER targeting sequence of hem…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    182645-plasmid-map-sequence-id-358618

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  4. pJS145

    Plasmid
    #179159
    Purpose
    Encodes 2xNLS::GFP(flexon), codon optimized for C. elegans. Blocks expression from ubiquitous promoter rps-27p with Flexon. Excision of flexon with Cre allows for strong expression.
    Depositor
    Iva Greenwald
    Article
    Shaffer et al Proc Natl Acad Sci U S A. 2022 Jan 18;11
    Insert
    rps-27p::2xNLS::GFP(flexon)::unc-54 3' UTR
    Use
    Tags
    Expression
    Worm
    Mutation
    Flexon replacing 2nd intron of 2xNLS::GFP
    Promoter
    rps-27p
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    179159-plasmid-map-sequence-id-352801

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  5. LiPOP1-NanoLuc

    Plasmid
    #168265
    Purpose
    Chemo-optogenetic control of necroptotic cell death by coupling LiPOP1 with NanoLuc-aided bioluminescence optogenetic stimulation (NanoLOGS)
    Depositor
    Yubin Zhou
    Article
    He et al Adv Sci (Weinh). 2021 May 6;8(13):210042
    Insert
    MLKL(1-125) (MLKL Human)
    Use
    Tags
    mCherry, NanoLuc, CRY2-PHR
    Expression
    Mammalian
    Mutation
    H15A, K16A, R17A
    Promoter
    CMV
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    168265-plasmid-map-sequence-id-334508

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  6. pB80-SSPB(micro)-GFP-GCN4-ppKin14VIb(861-1321)

    Plasmid
    #174640
    Purpose
    Optogenetic coupling to tetramerized moss kinesin-14 via SSPB(micro) for highly efficient retrograde transport
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    SSPB(micro)-GFP-GCN4-ppKinVIb(861-1321)
    Use
    Tags
    SSPB(micro)-GFP
    Expression
    Mammalian
    Mutation
    SSPB:Arg73Gln; EGFP: Met1Del; ppKin14VIb(aa861-13…
    Promoter
    Chicken beta-actin
    Available since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    174640-plasmid-map-sequence-id-347051

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  9. 3xFLAG-dCas9/pMSCVneo

    Plasmid
    #134982
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available since
    April 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    134982-plasmid-map-sequence-id-430627

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  10. pAD-KPS12-Pman

    Plasmid
    #133832
    Purpose
    Fluorescent protein mKate(KPS12) variant for optimized expression in Bacillus mycoides; under control of mannose inducible promoter.
    Depositor
    Oscar Kuipers
    Article
    Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57
    Insert
    mKate(KPS12)
    Use
    E.coli/bacillus spp. shuttle vector
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pman
    Available since
    Nov. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    133832-plasmid-map-sequence-id-261722

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  11. PXL

    Plasmid
    #75349
    Purpose
    PX330 derived. Bbs1 & annealed oligos to insert guide strand. BstB1+Pac1 of PXL and FUX-/pRubiX- T2A-Cas9 for choice of sgRNA, viral backbone, and fluorescent protein. Pac1 to introduce 2nd sgRNA.
    Depositor
    Bryan Luikart
    Article
    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10
    Insert
    Cas9
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    May 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    75349-plasmid-map-sequence-id-266810

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  13. 3xFLAG-dCas9/pMXs-neo

    Plasmid
    #51260
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available since
    Feb. 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    51260-plasmid-map-sequence-id-77871

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  15. pB80-SSPB(nano)-GFP-GCN4-ppKin14VIb(861-1321)

    Plasmid
    #174643
    Purpose
    Optogenetic coupling to tetramerized moss kinesin-14 via SSPB(micro) for highly efficient retrograde transport
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    SSPB(nano)-GFP-GCN4-ppKin14VIb(861-1321)
    Use
    Tags
    SSPB(nano)-GFP
    Expression
    Mammalian
    Mutation
    EGFP: Met1Del; ppKin14VIb(aa861-1321)
    Promoter
    Chicken beta-actin
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174643-plasmid-map-sequence-id-346994

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  18. mKate2-P2A-APEX2-ML1N*2

    Plasmid
    #67665
    Purpose
    Mammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain, [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.
    Depositor
    Rob Parton
    Article
    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:
    Insert
    mKate2-P2A-APEX2-ML1N*2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    67665-plasmid-map-sequence-id-117213

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  25. pAL204

    Plasmid
    #22282
    Purpose
    Expresses PhyB-mCFP with prenylation sequence from Kras, for red-light-induced recruitment of PIF to the plasma membrane
    Depositor
    Christopher Voigt
    Article
    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.
    Insert
    pCMV-PhyB(1-908)-10aaLinker-mCFP-Kras4BCT (PHYB Mustard Weed)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2010
    Availability
    Academic Institutions and Nonprofits only
    22282-plasmid-map-sequence-id-80604

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  28. pX330_TOMM20 sgRNA / hSpCas9

    Plasmid
    #172836
    Purpose
    Mammalian expression of a sgRNA targeting the intron 4 (last intron) of TOMM20 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting intron 4 of TOMM20 under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172836-plasmid-map-sequence-id-340903

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  29. CIBN-GFP-TMD

    Plasmid
    #79571
    Purpose
    expression of CIBN domain fused to GFP tagged mouse Syntaxin1 TMD domain (transmembrane domain and polybasic region)
    Depositor
    Olof Idevall-Hagren, Pietro De Camilli
    Article
    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10
    Insert
    syntaxin 1A (Stx1a Mouse)
    Use
    Tags
    CIBN and EGFP
    Expression
    Mammalian
    Mutation
    aa 249_288 (C-terminal region (polybasic stretch …
    Promoter
    CMV
    Available since
    July 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    79571-plasmid-map-sequence-id-456519

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  31. pK1E-RNAP

    Plasmid
    #48144
    Purpose
    Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage K1E under control of the xylose inducible promoter PxylA
    Depositor
    Dieter Jahn
    Article
    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2
    Insert
    rna polymerase K1E
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 21, 2013
    Availability
    Academic Institutions and Nonprofits only
    48144-plasmid-map-sequence-id-69927

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  34. 3xFLAG-dCas9/pMXs-IG

    Plasmid
    #51258
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    51258-plasmid-map-sequence-id-77869

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  38. pHM123DEnv

    Plasmid
    #137668
    Purpose
    Express HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)
    Depositor
    Anjali Joshi
    Article
    Joshi et al Virology. 2017 Dec;512:222-233. doi: 10.
    Insert
    HIV-1 Envelope Glycoprotein
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    137668-plasmid-map-sequence-id-273365

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Showing: 4801 - 4840 of 40591 results