We narrowed to 115,891 results for: mpi

1. pX551-miniCMV-SpCas9

   Plasmid

   #107031

   Purpose

   AAV plasmid expressing Cas9 under control of miniCMV promoter

   Depositor

   Alex Hewitt

   Insert

   SpCas9
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1-MS2-HA-HshnRNPR_AA

   Plasmid

   #148235

   Purpose

   Mammalian Expression of HshnRNPR

   Depositor

   Elisa Izaurralde

   Insert

   HshnRNPR (HNRNPR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Rich-pT7GG

   Plasmid

   #108443

   Purpose

   This plasmid is useful for in vitro transcription of genes using T7 promoter. Can be used for Xenopus oocyte assay. It is derived from pT7TS-rich. The vector is compatible for Golden-gate assembly

   Depositor

   Richard Bélanger

   Type

   Empty backbone

   Use

   Xenopus oocyte expression system

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAG35

   Plasmid

   #35123

   Depositor

   John McCusker

   Article

   Goldstein et al Yeast. 1999 Oct;15(14):1541-53.

   Insert

   natMX3
   

   Use

   Yeast replicating

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAG34

   Plasmid

   #35125

   Depositor

   John McCusker

   Article

   Goldstein et al Yeast. 1999 Oct;15(14):1541-53.

   Insert

   hphMX4
   

   Use

   Yeast replicating

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenti6MFV SLC40A1

   Plasmid

   #194132

   Purpose

   Expresses MTP1 (SLC40A1) protein in mammalian cells

   Depositor

   Jun Liu

   Insert

   SLC40A1 (SLC40A1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRDA_221

   Plasmid

   #169142

   Purpose

   Constitutive expression of EGFP and sgRNAs targeted to EGFP for measurement of activity of AsCas12a\EnAsCas12a.

   Depositor

   John Doench, David Root

   Insert

   EGFP , EGFP sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET-MBP-Geo_st

   Plasmid

   #87700

   Purpose

   Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP

   Depositor

   Jennifer Doudna

   Insert

   GeoCas9
   

   Use

   Tags

   10xHis-MBP-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3 mCherry LIC cloning vector (6B)

   Plasmid

   #30125

   Purpose

   Empty LIC vector; adds an mCherry gene to the C-terminus of your protein of interest.

   Depositor

   Scott Gradia

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LncRNA overexpression EF1α_BbsI_SV40 polyA_EGFP

    Plasmid

    #219828

    Purpose

    Overexpression vector for non-coding RNAs driven by EF1α promoter with GFP. Cloning using BbsI.

    Depositor

    Philipp Maass

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 31, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pRS327

    Plasmid

    #51787

    Purpose

    multicopy (YEp) vector with a LYS2 marker and a 2-μm origin of replication

    Depositor

    David Stillman

    Article

    Eriksson et al Biotechniques. 2004 Feb;36(2):212-3.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPB-CAG-dCas9-VPR-wpA-pPuroTK

    Plasmid

    #214127

    Purpose

    Gene expression by Piggybac transposase in mammalian cells

    Depositor

    Sho Yoshimatsu

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Codon-optimized for mammalian cells

    Promoter

    Available since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCS-CG

    Plasmid

    #12154

    Depositor

    Inder Verma

    Article

    Miyoshi et al J Virol. 1998 Oct . 72(10):8150-7.

    Insert

    CMV-GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-3'3xFlag

    Plasmid

    #208616

    Purpose

    Empty vector for mammalian expression, insert 3xFlag tag using BamHI andAflII site of pcDNA3.1 (-), both site retained, BamHI in-frame with 3xFlag

    Depositor

    Quanfu Ma

    Type

    Empty backbone

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pOpen-DNA Topoisomerase 1B Vaccinia Virus

    Plasmid

    #165510

    Purpose

    Used in TOPO cloning. Recognizes the DNA sequence 5'-(C/T)CCTT-3' and digests double stranded DNA at this sequence.

    Depositor

    Jennifer Molloy, Drew Endy, FreeGenes Project

    Insert

    DNA Topoisomerase 1B Vaccinia Virus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pLIX403-iRFP-ccdB-Puro

    Plasmid

    #158566

    Purpose

    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    iRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. IDG_SCN2B_OE_1

    Plasmid

    #161624

    Purpose

    Overexpresses SCN2B

    Depositor

    Mike McManus

    Insert

    SCN2B (SCN2B Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. IDG_SCN3B_OE_1

    Plasmid

    #161625

    Purpose

    Overexpresses SCN3B

    Depositor

    Mike McManus

    Insert

    SCN3B (SCN3B Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TMC1-TwinStrepII

    Plasmid

    #83267

    Purpose

    Expression of tagged protein in mammalian cells

    Depositor

    Peter Barr-Gillespie

    Insert

    TMC1 (Tmc1 Mouse)
    

    Use

    Tags

    Twin Strep Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-EGFP-p50

    Plasmid

    #107977

    Purpose

    Expresssion of NF-kB p50

    Depositor

    Georg Weber

    Insert

    NF-kB p50 (Nfkb1 Mouse)
    

    Use

    Tags

    N/A

    Expression

    Mammalian

    Mutation

    Amino acids VQACI added to C-terminus

    Promoter

    CMV

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTipi2.1

    Plasmid

    #225877

    Purpose

    Allows for expression of recombinant antibodies

    Depositor

    Institute for Protein Innovation (IPI)

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 23, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. pFastBac Dual His6 MBP N10 TEV LIC cloning vector (5C)

    Plasmid

    #30123

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6-MBP-N10-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCAG-N2cG

    Plasmid

    #100811

    Purpose

    express rabies virus CVS N2c strain glycoprotein gene (N2cG)

    Depositor

    Ian Wickersham

    Insert

    rabies virus CVS N2c strain glycoprotein gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSB-Bleo Gal4 tBFP

    Plasmid

    #139190

    Purpose

    Gal4-inducible tag blue fluorescent protein (tBFP) in pSB-Bleo Gal4 Sleeping Beauty backbone.

    Depositor

    Neal Devaraj

    Insert

    tBFP
    

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Gal4 CMV and STAT3

    Available since

    July 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1 Calreticulin

    Plasmid

    #191940

    Purpose

    Expression of calreticulin

    Depositor

    Luca Scorrano

    Insert

    Calreticulin (Calr Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. 1245 pCMVb p300

    Plasmid

    #10717

    Depositor

    William Sellers

    Insert

    p300 (EP300 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.1-StrepII

    Plasmid

    #208617

    Purpose

    Empty vector for mammalian expression, insert a Strep II tag (with ATG) using XbaI and XhoI site of pcDNA3.1 (-), both site retained

    Depositor

    Quanfu Ma

    Type

    Empty backbone

    Use

    Tags

    Strep II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMB-Tl-ADPHK

    Plasmid

    #237469

    Purpose

    Expression of His-tagged Thermococcus litoralis ADP hexokinase

    Depositor

    Michael Baker

    Insert

    ADP hexokinase
    

    Use

    Tags

    C-terminal 10 His-tag and N-terminal strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. lenti-RfxCas13d-Blast-BFP

    Plasmid

    #196726

    Purpose

    Sortable and selectable RfxCas13d.

    Depositor

    Bernhard Schmierer

    Insert

    RfxCas13d-T2A-BSD-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    SV40 NLS

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. hSYN-mito-dsRED2

    Plasmid

    #173069

    Purpose

    mitochondria targeted dsRED2 expressed off of human Synapsin I promoter.

    Depositor

    Thomas Schwarz

    Insert

    Mito
    

    Use

    Lentiviral

    Tags

    dsRED

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin 1

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBac[3xP3-DsRed; UAS-Tc'hsp_p-tGFP-SV40]

    Plasmid

    #86453

    Purpose

    UAS responder construct for misexpression in Tribolium; UAS drives tGFP expression; (Transformation plasmid; Red eye marker)

    Depositor

    Gregor Bucher

    Insert

    3xP3-DsRed; UAS-Tc'hsp_p-tGFP-SV40
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pPGK-Cre-bpA

    Plasmid

    #11543

    Purpose

    Expresses Cre recombinase from mouse PGK promoter in mammalian cells.

    Depositor

    Klaus Rajewsky

    Insert

    Phosphoglycerate kinase 1 promoter
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse PGK

    Available since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET15-MHL

    Plasmid

    #26092

    Purpose

    SGC Empty backbone for bacterial expression under T7 promoter. Uses infusion based cloning method.

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Tags

    6xHis and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    Sept. 6, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. mem-mCherry-pTRIP

    Plasmid

    #163521

    Purpose

    Used to construct lentivirus to express membrane-targeted mCherry in mammalian cells

    Depositor

    Thomas Weber

    Insert

    membrane targeted mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pRetroQ-Cre-ERT2

    Plasmid

    #59701

    Purpose

    to make Cre-ERT2 expressing stable cell line by retrovirus

    Depositor

    Richard Youle

    Insert

    Cre-ERT2
    

    Use

    Retroviral

    Tags

    Estrogen receptor (ERT)

    Expression

    Mammalian

    Mutation

    Promoter

    cmvIE

    Available since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Col1A2:TFPnls-T2a-mErt-Cre-Ert

    Plasmid

    #111151

    Purpose

    Fibroblasts specific inducible Cre

    Depositor

    Elly Tanaka

    Insert

    TFPnls-T2a-mErt-Cre-Ert
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    wt

    Promoter

    Col1A2

    Available since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCMV-STAT6-IRES-Neo

    Plasmid

    #35482

    Depositor

    Axel Nohturfft

    Insert

    STAT6 (STAT6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAS_4xMmaPylT_EF1_mCherry_TAG_EGFP

    Plasmid

    #174893

    Purpose

    amber suppression reporter mCherry-TAG-EGFP expression, with MmaPylT amber suppressor tRNA cassette

    Depositor

    Simon Elsaesser

    Insert

    mCherry and EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. AbVec2.0-mIghg2c

    Plasmid

    #127159

    Purpose

    Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG2c isotype

    Depositor

    Hedda Wardemann

    Insert

    Ighg2c (Ighg2c Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV IE1 gene promoter

    Available since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAG32

    Plasmid

    #35122

    Depositor

    John McCusker

    Article

    Goldstein et al Yeast. 1999 Oct;15(14):1541-53.

    Insert

    hphMX4
    

    Use

    Yeast replicating

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCMV-ABE8e-NRCH

    Plasmid

    #165416

    Purpose

    Expresses the base editor ABE8e-NRCH in mammalian cells

    Depositor

    David Liu

    Insert

    ABE8e-NRCH
    

    Use

    Tags

    BP-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. FUGW-VGLUT1-pHluorin

    Plasmid

    #207470

    Purpose

    Lentiviral expression of VGLUT1-pHluorin

    Depositor

    Robert Edwards

    Insert

    VGLUT1 (Slc17a7 Rat)
    

    Use

    Lentiviral

    Tags

    pHluorin

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCSFLPe

    Plasmid

    #31130

    Depositor

    Gerhart Ryffel

    Article

    Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

    Insert

    FLP recombinase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pLinker-6xHA-HXGPRT-LoxP

    Plasmid

    #86552

    Purpose

    The plasmid is used for endogenous tagging using CRISPR technology in Toxoplasma

    Depositor

    David Sibley

    Insert

    Linker-6xHA-HGPRT-LoxP
    

    Use

    Expression in toxoplasma gondii

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pAAV-GFAP-hChR2(H134R)-mCherry

    Plasmid

    #27055

    Purpose

    AAV expression of humanized ChR2(H134R) fused to mCherry, for optogenetic excitation

    Depositor

    Karl Deisseroth

    Insert

    hChR2(H134R)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    GFAP

    Available since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pExp-Trx

    Plasmid

    #112577

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Trx (thioredoxin (trxA) from E. coli fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis-TrxA and Strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pAG CMV N-HA Puro

    Plasmid

    #237879

    Purpose

    Empty mammalian expression plasmid for cloning N-terminally HA-tagged proteins

    Depositor

    Meghan Rego, Addgene Research Program

    Type

    Empty backbone

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 12, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

48. LZRS-Rfa

    Plasmid

    #31601

    Depositor

    Paul Khavari

    Article

    Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pAG25

    Plasmid

    #35121

    Depositor

    John McCusker

    Article

    Goldstein et al Yeast. 1999 Oct;15(14):1541-53.

    Insert

    natMX4
    

    Use

    Yeast replicating

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. FLX1.8CMVMitoTurboRFP

    Plasmid

    #28131

    Depositor

    Geoffrey Owens

    Article

    Chen et al PLoS One. 2008 . 3(7):e2804.

    Insert

    MitoTurboRFP
    

    Use

    Lentiviral

    Tags

    COX VIII signal sequence

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge