We narrowed to 5,046 results for: codon optimized

1. pT3TS_Transposase

   Plasmid

   #109768

   Purpose

   Codon optimised transposase sequence in an optimal vector for in vitro transcription. Contains the T3 promoter and synthetic polyA tract.

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   Tol2 Transposase
   

   Use

   Zebrafish plasmids

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. His-NavMs

   Plasmid

   #100004

   Purpose

   Electrophysiology in mammalian cells: Mammalian expression of voltage gated sodium channel and GFP

   Depositor

   Paul DeCaen

   Article

   Sula et al Nat Commun. 2017 Feb 16;8:14205. doi: 10

   Insert

   His-NavMs, Voltage gated sodium channel
   

   Use

   Tags

   Hexa-His tag followed by a thrombin cleavage site

   Expression

   Mammalian

   Mutation

   C-terminus from a codon-optimized synthetic gene,…

   Promoter

   CMV

   Available since

   Aug. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMK365 (Tet-OsTIR1-loxP-PURO-loxP)

   Plasmid

   #121185

   Purpose

   OsTIR1 introduction

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

   Insert

   Tet-OsTIR1-loxP-PURO-loxP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   OsTIR1 codon optimized for human cells

   Promoter

   Available since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR221_SLC22A1

   Plasmid

   #132034

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Mocking et al Int J Mol Sci. 2022 Jan 21;23(3):1203. d

   Insert

   SLC22A1 (SLC22A1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 25, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pUB-Cas9

   Plasmid

   #86556

   Purpose

   For ubiquitous expression of Cas9 in planta. sgRNA can be introduced as described in Hahn et al. (2017)

   Depositor

   Andreas Weber

   Article

   Hahn et al Front Plant Sci. 2017 Jan 24;8:39. doi:

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Cas9 is codon optimized for C. reinhardtii

   Promoter

   UBIQUITIN10

   Available since

   May 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSL2620 (pTniQ_Vch)

   Plasmid

   #200892

   Purpose

   Expresses human codon-optimized V. cholerae TniQ from a CMV promoter.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   VchTniQ
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSCL.139

   Plasmid

   #184994

   Purpose

   Express Cas9-P2A-Eco1RT in human cells

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   SpCas9-P2A-Eco1RT
   

   Use

   Tags

   SV40NLS

   Expression

   Mammalian

   Mutation

   Human codon optimized RT

   Promoter

   TetOn-3g

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. C1-FT-mCitrine-EEVEE-CaaX

   Plasmid

   #155225

   Purpose

   MPAct with increased linker flexiblity

   Depositor

   Tobias Meyer

   Article

   Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

   Insert

   F-tractin (aa 9-52 of rat ITPKA) fused to mCitrine C-term tagged with a codon optimized EEVEE and CaaX seq derived from Kras4b
   

   Use

   Tags

   mCitrine

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSSM16: pCMV-HA-NLS-SpuFz1

   Plasmid

   #205271

   Purpose

   Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1

   Depositor

   Feng Zhang

   Article

   Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

   Insert

   SpuFz1
   

   Use

   Tags

   HA, NLS(Makkerh)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. 3xFLAG-dCas9/pMSCVneo

    Plasmid

    #134982

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR and Retroviral

    Tags

    3xFLAG tag and NLS

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    LTR

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. 3xFLAG-dCas9/pMSCVpuro

    Plasmid

    #134983

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR and Retroviral

    Tags

    3xFLAG tag and NLS

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    LTR

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330 EGFR-sgRNA

    Plasmid

    #188633

    Purpose

    A human codon-optimized SpCas9 and chimeric guide RNA targeting C-terminus of human EGFR

    Depositor

    Jared Toettcher

    Article

    Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS2+_Flag-myc-NES-Tir1

    Plasmid

    #117717

    Purpose

    mRNA synthesis of Flag-myc-NES-TIR1 for injection

    Depositor

    Joerg Mansfeld

    Article

    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

    Insert

    TRANSPORT INHIBITOR RESPONSE 1
    

    Use

    Tags

    Flag-Myc-NES

    Expression

    Mammalian

    Mutation

    codon optimized TIR1 for murine expression

    Promoter

    SP6

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. MDO1-c014

    Plasmid

    #173103

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    MACROD1 (MACROD1 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    S58-A325, codon optimized

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. P628_SpCas9-RecA

    Plasmid

    #87263

    Purpose

    Human codon optimized RecA protein was fused to SpCas9 for enhanced genome editing efficiency

    Depositor

    Yonglun Luo

    Article

    Lin et al J Biotechnol. 2017 Mar 1;247:42-49. doi:

    Insert

    RecA
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    no

    Promoter

    CBh

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIntegrase_7

    Plasmid

    #60578

    Purpose

    plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Integrase_7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pBAD

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTU398

    Plasmid

    #178980

    Purpose

    13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization)

    Depositor

    Stan Brouns

    Article

    van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

    Insert

    gRAMP
    

    Use

    Tags

    Strep-Tag II SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. Gi-c001

    Plasmid

    #173078

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    GNAI1 (GNAI1 Human)
    

    Use

    Tags

    mCitrine

    Expression

    Bacterial

    Mutation

    codon optimized

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pACT2-CAS9c

    Plasmid

    #51055

    Purpose

    Express Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 promoter for genome editing. SV40 NLS is fused to the C terminal. GAL4 region from pACT2 is removed.

    Depositor

    Yunde Zhao

    Article

    Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

    Insert

    Cas9c
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    yeast ADH1 promoter

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. ARH2-c003

    Plasmid

    #173094

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    ADPRHL1 (ADPRHL1 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    E2-K354, codon optimized

    Promoter

    Available since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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