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pMS4 (Empty insertion vector with SEC)

Plasmid

#154837

Purpose

Vector that can be modified to add gene(s) of interest to C. elegans ChrII:8420157 site via CRISPR with SEC selection

Depositor

Patrick Phillips

Article

Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

Insert

SEC

Use

CRISPR and Cre/Lox

Expression

Worm

Mutation

sqt-1(e1350)

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-Flex-FlpO

Plasmid

#191202

Purpose

Cre dependent FlpO expression

Depositor

Janelia Viral Tools

Article

Winnubst et al Cell. 2019 Sep 19;179(1):268-281.e13. do

Insert

FlpO

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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ZJOM141

Plasmid

#133669

Purpose

Integration of COX9-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast mitochondria.

Depositor

Zhiping Xie

Article

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

p1k-COX9-mTagBFP2 (COX9 Budding Yeast)

Tags

mTagBFP2

Expression

Yeast

Available Since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pEMS2158

Plasmid

#70119

Purpose

Promoterless AAV plasmid with MCS to insert promoter for expression of EmGFP.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-MCS-EmGFP

Use

AAV

Expression

Mammalian

Promoter

Promoterless

Available Since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pEMS1980

Plasmid

#49111

Purpose

Promoterless AAV plasmid with MCS to insert promoter for expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-MCS-iCre

Use

AAV

Expression

Mammalian

Promoter

Promoterless

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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MSGV Rh Acceptor PGK-NGFR

Plasmid

#64272

Purpose

Retroviral golden gate cloning vector for expression of functional Rhesus T-Cell Receptor genes with NGFR

Depositor

David Ott

Article

Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

Type

Empty backbone

Use

Retroviral

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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miniSOG-Rab5a-7

Plasmid

#57774

Purpose

Localization: Ras GTPase, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Rab5a

Tags

miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1-K20

Plasmid

#14743

Depositor

M Bishr Omary

Article

Zhou et al Mol Biol Cell. 2003 Jul . 14(7):2959-71.

Insert

Keratin 20 (KRT20 Human)

Expression

Mammalian

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pHH0103_GNAI3_G-alpha

Plasmid

#110014

Purpose

Protein expression and purification of GNAI3_G-alpha

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

GNAI3_G-alpha (GNAI3 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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px551-mSncg-0.27kb-HA-SpCas9

Plasmid

#153208

Purpose

Truncated Mouse gamma-synuclein (mSncg-0.27kb) promoter-mediates HA and Cas9 expression in retinal ganglion cells

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Insert

Cas9

Use

AAV and CRISPR

Tags

HA

Expression

Mammalian

Promoter

Truncated mSncg (0.27kb)

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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ZJOM96

Plasmid

#133663

Purpose

Integration of SEC26-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast early golgi.

Depositor

Zhiping Xie

Article

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

p1k-SEC26-mTagBFP2 (SEC26 Budding Yeast)

Tags

mTagBFP2

Expression

Yeast

Available Since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-Crispr-V2-HMID.v1

Plasmid

#103062

Purpose

LentiCrisprV2 with guide targeting V1-V5 GESTALT barcodes

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

Cas9 plus expressed GESTALT V1-V5 guide

Use

Lentiviral

Available Since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_ESRRA_Hormone-recep

Plasmid

#109908

Purpose

Protein expression and purification of ESRRA_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

ESRRA_Hormone-recep (ESRRA Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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ZJOM157

Plasmid

#133640

Purpose

Integration of GFP-PEP12 as an additional copy in genome, use auxotrophic marker URA3(Kluyveromyces lactis).Marker for yeast late endosome.

Depositor

Zhiping Xie

Article

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

p1k-GFP-Pep12 (PEP12 Budding Yeast)

Tags

EGFP

Expression

Yeast

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pACP-GPI Control Plasmid

Plasmid

#101131

Purpose

Expression of ACP-Tagged GPI anchor in Mammalian cells

Depositor

New England Biolabs, Ana Egana

Insert

GPI

Tags

ACP-Tag (ACP wt)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pMLS286

Plasmid

#73723

Purpose

Saptrap tagRFP + Cbr-unc-119 donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

tagRFP + Cbr-unc-119

Use

CRISPR and Cre/Lox

Expression

Worm

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pMLS291

Plasmid

#73724

Purpose

SapTrap mCherry + Cbr-unc-119 donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

mCherry + Cbr-unc-119

Use

CRISPR and Cre/Lox

Expression

Worm

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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mKate2-P2A-APEX2-2xML1N(7Q)

Plasmid

#67666

Purpose

Mammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain (mutant version), [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

mKate2-P2A-APEX2-ML1Nmut*2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR Age HsSAS-6 ST

Plasmid

#46382

Purpose

For expression of N-terminal fusions.

Depositor

Pierre Gonczy

Article

Kitagawa et al Cell. 2011 Feb 4;144(3):364-75. doi: 10.

Insert

SAS-6 (SASS6 Human)

Use

Gateway Cloning

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pCFJ895 - pEXP5605(cbr-unc-119)[Psmu-1 |smu-1 genomic | GFP-smu-1UTR]

Plasmid

#159817

Purpose

Positive control for germline GFP expression from simple array in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP5605(cbr-unc-119)[Psmu-1 |smu-1 genomic | GFP-smu-1UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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