Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 14,191 results for: CAN

Showing: 2421 - 2440 of 14191 results

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sgMAPKAP1_3

Plasmid

#124913

Purpose

Targeting human MAPKAP1 (SIN1) gene by CRISPR-Cas9

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MAPKAP1 (MAPKAP1 Human)

Use

CRISPR and Lentiviral

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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KB501: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; CMV promoter

Plasmid

#121813

Purpose

pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and CMV promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Gateway Cloning and Synthetic Biology

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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LSB-hsa-miR-302a-3p

Plasmid

#103400

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-302a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-302a-3p target (MIR302A Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF-1a

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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LSB-hsa-miR-885-3p

Plasmid

#103744

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-885-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-885-3p target (MIR885 Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF-1a

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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LSB-hsa-miR-579-5p

Plasmid

#103661

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-579-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-579-5p target (MIR579 Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF-1a

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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LSB-hsa-miR-510-5p

Plasmid

#103593

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-510-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-510-5p target (MIR510 Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF-1a

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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LSB-hsa-miR-495-3p

Plasmid

#103568

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-495-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-495-3p target (MIR495 Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF-1a

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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LSB-hsa-miR-26a-1-3p

Plasmid

#103377

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-26a-1-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-26a-1-3p target (MIR26A1 Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF-1a

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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LSB-hsa-miR-26a-2-3p

Plasmid

#103378

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-26a-2-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-26a-2-3p target (MIR26A2 Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF-1a

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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LSB-hsa-miR-483-3p

Plasmid

#103550

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-483-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-483-3p target (MIR483 Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF-1a

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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LSB-hsa-miR-31-3p

Plasmid

#103420

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-31-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-31-3p target (MIR31 Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF-1a

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-miR-96-5p

Plasmid

#103761

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-96-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-96-5p target (MIR96 Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF-1a

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-miR-122a-5p

Plasmid

#103183

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-122a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-122a-5p target (MIR122 Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF-1a

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-miR-126-5p

Plasmid

#103193

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-126-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-126-5p target (MIR126 Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF-1a

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIBN-TetR-tagRFP-T

Plasmid

#103809

Purpose

'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-TetR-tagRFP-T (CIB1, tetR Mustard Weed, E. coli)

Expression

Mammalian

Mutation

TetR: A4G (M2V)

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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KB601: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; EF1a promoter

Plasmid

#121819

Purpose

pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Gateway Cloning and Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-let-7f-2-3p

Plasmid

#103155

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-let-7f-2-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-let-7f-2-3p target (MIRLET7F2 Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF-1a

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-miR-93-3p

Plasmid

#103755

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-93-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-93-3p target (MIR93 Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF-1a

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-miR-34a-3p

Plasmid

#103464

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-34a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-34a-3p target (MIR345 Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF-1a

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-miR-145-5p

Plasmid

#103246

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-145-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-145-5p target (MIR145 Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF-1a

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 2421 - 2440 of 14191 results

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