We narrowed to 2,493 results for: ERK
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pJCC_103 SpCas9 T1337C
Plasmid#179525PurposeFor bacterial expression of SpCas9 T1337C (for DNA cross-linking) with an N-terminal His-MBP tagDepositorInsertSpCas9 T1337C
UseTags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged threonine 1337 to cysteinePromoterAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
Sc NybbR eRF3∆165N (H348E)
Plasmid#175420PurposeEncodes His-MBP-TEV-ybbR-H348E eRF3∆165NDepositorInserteRF3 (SUP35 Budding Yeast)
UseTagsHis-MBP-TEV and ybbRExpressionBacterialMutation∆165N, H348EPromoterT7Available sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pCDNA3 Flag MKK4
Plasmid#14615DepositorInsertMKK4 (MAP2K4 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag MKK4(glu)
Plasmid#14813DepositorInsertMKK4(glu) (MAP2K4 Human)
UseTagsFlagExpressionMammalianMutationSer-220Glu Thr-224GluPromoterAvailable sinceJuly 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag MKK4(ala)
Plasmid#15314DepositorInsertMKK4(ala) (MAP2K4 Human)
UseTagsFlagExpressionMammalianMutationSer-220Ala Thr-224AlaPromoterAvailable sinceJuly 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTA231-p.Syn-PAmCherry1Hfx
Plasmid#164661Purposeexpression of H. volcanii codon-optimized PAmCherry1DepositorInsertPAmCherry1Hfx
UseArchaeal expressionTagsExpressionMutationcodon-optimization for Haloferax volcaniiPromoterp.SynAvailable sinceFeb. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag MKK4beta
Plasmid#15517DepositorInsertpCDNA3 Flag MKK4beta (MAP2K4 Human)
UseTagsFlagExpressionBacterialMutationPromoterAvailable sinceFeb. 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTA231-p.Syn-Dendra2Hfx
Plasmid#164660Purposeexpression of H. volcanii codon-optimized Dendra2DepositorInsertDendra2Hfx
UseArchaeal expressionTagsExpressionMutationcodon-optimization for Haloferax volcaniiPromoterp.SynAvailable sinceFeb. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAC-luc-CLK
Plasmid#33132DepositorInsertCLK 3' UTR (Clk Fly)
UseLuciferaseTagsFirefly LuciferaseExpressionInsectMutationPromoterActin 5CAvailable sinceJan. 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
HA SEK AL pcDNA3.1 zeo (+) - SEK1 (A-L)
Plasmid#24653DepositorInsertSEK1 (MAP2K4 Human)
UseTagsHAExpressionMammalianMutationSer220Ala, Thr224LeuPromoterAvailable sinceJune 21, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJCC_162 SpCas9 KES(1107-1109)GG
Plasmid#179526PurposeFor bacterial expression of SpCas9 KES(1107-1109)GG (phosphate lock loop mutant) with an N-terminal His-MBP tagDepositorInsertSpCas9 KES(1107-1109)GG
UseTags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationreplaced KES (residues 1107-1109) with GGPromoterAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJCC_164 SpCas9 R1333A/R1335A
Plasmid#179528PurposeFor bacterial expression of SpCas9 R1333A/R1335A (PAM-binding arginines mutant) with an N-terminal His-MBP tagDepositorInsertSpCas9 R1333A/R1335A
UseTags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged arginines 1333 and 1335 to alaninePromoterAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
EcRfaHKOW-L1L2NusG
Plasmid#113126PurposeExpresses C-terminus of E. coli RfaH with L1 and L2 loops replaced with NusG residuesDepositorInsertRfaH (rfaH Escherichia coli)
UseTagsHis6-MBP-N10-TEVExpressionBacterialMutationDeleted residues 1-107, replaced L1 and L2 loops …PromoterT7Available sinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRM055 His-TEV-Cas9 6xNLS 0C
Plasmid#196246PurposeExpression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)DepositorInsertCas9
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPBJ pFOS-KTR-Gal4-PEST-tub3’ pCMV-TagBFP
Plasmid#176531PurposePiggyBAC vector expressing a FOS promoter driven "KGV" synthetic transcription factor and a CMV promoter driven BFP marker.DepositorInsertsKGV transcription factor
TagBFP
UsePiggybac transposase integration vectorTagsErkKTR, Gal4 DNA binding domain, PEST destabiliza…ExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBC2T-Ypet-FtnA
Plasmid#108969Purposeexpresses BC2-tag-Ypet-FtnA in mammalian cellsDepositorInsertFtnA (ftnA Escherichia coli)
UseTagsBC2-tag and YpetExpressionMammalianMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJCC_163 SpCas9 K233A/K234A/K253A/K263A
Plasmid#179527PurposeFor bacterial expression of SpCas9 K233A/K234A/K253A/K263A (helix-rolling basic patch mutant) with an N-terminal His-MBP tagDepositorInsertSpCas9 K233A/K234A/K253A/K263A
UseTags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged lysines 233, 234, 253, and 263 to alaninePromoterAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only
