We narrowed to 18,379 results for: Met

1. Tus pGST-//

   Plasmid

   #53305

   Purpose

   Expresses GST-tagged E.coli replication terminator protein Tus.

   Depositor

   John Lis

   Article

   Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

   Insert

   E.Coli Tus replication terminator protein
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. CFP-tTA-ER-C3

   Plasmid

   #134569

   Purpose

   Expresses a CFP-tTA (tetracycline transcriptional activator)-ER (estrogen receptor hormone binding domain) fusion protein

   Depositor

   Susan Janicki

   Article

   Rafalska-Metcalf et al PLoS One. 2010 Apr 21;5(4):e10272. doi:

   Insert

   tTA-ER
   

   Tags

   CFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQE30del

   Plasmid

   #106902

   Purpose

   pQE30del is conditionally replicating empty vector. Replication of the plasmid is inhibited in presense of IPTG or lactose. pQE30del can be used for construction of any E. coli helper plasmids.

   Depositor

   Sergey Sineoky

   Article

   Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   June 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti-sgNT3/Cre

   Plasmid

   #89654

   Purpose

   Expresses a non-targeting gRNA and Cre-recombinase

   Depositor

   Monte Winslow

   Article

   Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

   Insert

   non-targeting gRNA
   

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Available Since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLEX_307_ms2_VPR

   Plasmid

   #79376

   Purpose

   Recruits VPR to compatible gRNAs for transcriptional activation

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   ms2-VPR
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBCN27-Pmyo-2::GFP::unc-54_3'UTR

   Plasmid

   #26347

   Depositor

   Ben Lehner

   Article

   Semple et al Nat Methods. 2010 Sep;7(9):725-7. doi: 1

   Insert

   Pmyo-2::GFP::unc-54_3'UTR
   

   Expression

   Worm

   Available Since

   Dec. 6, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV_mCherry_2A_HA_2xMS2-VP64

   Plasmid

   #68419

   Purpose

   Transient expression of humanized MS2 (V75E/A81G), fused to the VP64 transcrption activator, in mammalian cells, under a CMV promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   MS2
   

   Use

   Synthetic Biology

   Tags

   HA and VP64

   Expression

   Mammalian

   Mutation

   MS2: V75E/A81G

   Promoter

   CMV

   Available Since

   Sept. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMito-iRFP720

   Plasmid

   #45464

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

   Insert

   iRFP720
   

   Tags

   human cytochrome C oxidase subunit VIII

   Expression

   Mammalian

   Mutation

   T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…

   Promoter

   CMV

   Available Since

   June 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJMK004

   Plasmid

   #34616

   Purpose

   Viral expression of Arch (D95N mutant with greater sensitivity but slower response) voltage indicator

   Depositor

   Adam Cohen

   Article

   Kralj et al Nat Methods. 2011 Nov 27. doi: 10.1038/n

   Insert

   Arch D95N
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Changed Aspartic Acid 95 to Asparagine

   Promoter

   CaMKII

   Available Since

   Jan. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIntegrase_7

    Plasmid

    #60578

    Purpose

    plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Integrase_7
    

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pBAD

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pZ8-P_dCas9

    Plasmid

    #74063

    Purpose

    pZ8-1 plasmid carrying dcas9 driven by the propionate-inducible prpD2 promoter (PprpD2), KanR

    Depositor

    Timothy Lu

    Article

    Cleto et al ACS Synth Biol. 2016 Feb 16.

    Insert

    dcas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    Propionate inducible promoter (prp)

    Available Since

    April 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMK377 (Hygro-P2A-mCherry2)

    Plasmid

    #121188

    Purpose

    N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    Hygro-P2A-mCherry2
    

    Use

    CRISPR

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. p1xNLS-FRB-mCherry-hsp86-BSD

    Plasmid

    #85795

    Purpose

    Expression of 1xNLS mislocaliser in P. falciparum

    Depositor

    Tobias Spielmann

    Article

    Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    1xNLS Mislocaliser
    

    Use

    P. falciparum expression

    Tags

    FRB*, mCherry

    Promoter

    Pfhsp86

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCFJ906

    Plasmid

    #44472

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Insert

    Minimal Mos1, unc-119, [4-3] Gateway
    

    Use

    Gateway destination vector

    Available Since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEB1-mCerulean3

    Plasmid

    #103973

    Purpose

    Plasmid encoding mCerulean3

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mCerulean3
    

    Use

    Low copy

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (F64L, Y66W, …

    Promoter

    proC

    Available Since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLEX_307_ms2_p65_hsf1

    Plasmid

    #79374

    Purpose

    Recruits p65_hsf1 to compatible gRNAs for transcriptional activation

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    ms2-p65-hsf1
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Sept. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. XW09

    Plasmid

    #65833

    Purpose

    Punc-4c-QS-SL2-mcherry3'UTR-unc-54

    Depositor

    Kang Shen

    Article

    Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

    Insert

    Neurospora crassa OR74A repressor (qa-1S)
    

    Tags

    mCherry

    Expression

    Worm

    Promoter

    Punc-4c

    Available Since

    Jan. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEB2-DsRed-Express

    Plasmid

    #103995

    Purpose

    Plasmid encoding DsRed-Express

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    DsRed-Express
    

    Use

    Low copy

    Expression

    Bacterial

    Mutation

    Mutations relative to DsRed (R2A, K5E, N6D, T21S,…

    Promoter

    proC

    Available Since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. ciCas9_pcDNA5

    Plasmid

    #100550

    Purpose

    Expresses ciCas9 in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

    Insert

    ciCas9
    

    Use

    CRISPR; Frt, flp-in

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEO100c

    Plasmid

    #50550

    Purpose

    Expresses sfGFP under the PompC1157 promoter

    Depositor

    Jeffrey Tabor

    Article

    Olson et al Nat Methods. 2014 Mar 9. doi: 10.1038/nm

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    May 22, 2014

    Availability

    Academic Institutions and Nonprofits only