We narrowed to 4,709 results for: lum

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Tet-pLKO-puro shPOLE1_1

Plasmid

#160810

Purpose

Express Dox repressible shPOLE1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shPOLE1_1

Use

Lentiviral

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3_erGAP3

Plasmid

#78118

Purpose

Low affinity fluorescent calcium indicator of the GAP (GFP-Aequorin Protein) family targeted to the endoplasmic reticulum

Depositor

Teresa Alonso, Javier García-Sancho

Article

Navas-Navarro et al Cell Chem Biol. 2016 Jun 1. pii: S2451-9

Insert

erGAP3

Tags

KDEL and calreticulin signal peptide

Expression

Mammalian

Mutation

S175G, D180Y, I167V and L15Q in GFP moiety and D…

Promoter

CMV

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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Rtn4a-GFP

Plasmid

#61807

Purpose

Human Rtn4a fluorescently tagged with GFP on the C-terminus for mammalian expression

Depositor

Gia Voeltz

Article

Shibata et al J Biol Chem. 2008 Jul 4. 283(27):18892-9

Insert

Rtn4a (RTN4 Human)

Tags

AcGFP

Expression

Mammalian

Mutation

Rtn4a Stop Codon removed

Promoter

CMV

Available Since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

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TetON-shYAP

Plasmid

#115667

Purpose

Inducible YAP knockdown

Depositor

Joan Massague

Article

Er et al Nat Cell Biol. 2018 Aug;20(8):966-978. d

Insert

YAP shRNA (YAP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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GFP scFv [N86/38]

Plasmid

#182095

Purpose

Mammalian Expression of GFP scFv. Derived from hybridoma N86/38.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting GFP

Tags

HA, His

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-SFFV-KTN1-WPRE-UbC-Emerald

Plasmid

#225941

Purpose

Lentiviral vector plasmid expressing human kinectin 1 (KNT1) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

Depositor

Richard Eva, Bart Nieuwenhuis, Evan Reid

Inserts

KTN1 (KTN1 Human)
Emerald

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV and UbC

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSicoR-Ef1a-mCh-Puro

Plasmid

#31845

Purpose

Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression. Includes puromycin selection.

Depositor

Bruce Conklin

Article

Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

Type

Empty backbone

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

U6 for shRNA; Ef1alpha for mCherry-puromycin

Available Since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pNK5785

Plasmid

#219753

Purpose

pGAP-like vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 under control of GAP promoter, for yeast expression

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

pGAP - nnLuz_v4 - tAOX

Use

Luciferase

Expression

Yeast

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAd-Track Flag HA PGC-1 alpha

Plasmid

#14426

Depositor

Pere Puigserver

Article

Lerin et al Cell Metab. 2006 Jun . 3(6):429-38.

Insert

PGC-1 alpha (Ppargc1a Mouse)

Use

Adenoviral

Tags

Flag and HA

Expression

Mammalian

Mutation

Last four amino acids of PGC-1a removed to make t…

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
pMuLE_EXPR_CMV-eGFP_TOP-NLuc1.1_12GLI-FLuc_CBF-GLuc

Plasmid

#113862

Purpose

Triple pathway reporter, 3P-Luc; wnt-NLuc1.1, hedgehog-FLuc, notch-GLuc; plus CMV-eGFP. Gateway expression vector for lentivirus generation.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Inserts

CMV-eGFP
TOP-NLuc1.1
12GLI-Fluc
CBF-GLuc

Use

Lentiviral

Expression

Mammalian

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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CHOP promoter (-649/+136) pmCherry-1

Plasmid

#36035

Depositor

Quan Lu

Article

Oh et al J Biol Chem. 2012 Feb 17;287(8):6025-34.

Insert

CHOP promoter (-647 to +136) (DDIT3 Human)

Expression

Mammalian

Available Since

Aug. 7, 2012

Availability

Academic Institutions and Nonprofits only

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MSCV-opEBNA2-IRES-mCherry

Plasmid

#220355

Purpose

Expresses EBV latent gene EBNA2 in mammalian cells (EBNA2 coding sequence was optimised for expression)

Depositor

Klaus Rajewsky

Article

Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

Insert

opEBNA2 (EBNA-2 Epstein-Barr Virus (EBV) strain B95-8)

Use

Retroviral

Expression

Mammalian

Mutation

codon-optimised for expression in mammalian cells

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMSR:MSMEG_0709:Dendra

Plasmid

#159077

Purpose

T7 expression of C-term fluorescent fusion in E. coli

Depositor

Keith Derbyshire, Joseph Wade

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_0709

Use

Integrating mycobacterial expression vector attl5

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Promoter

pmycTetO

Available Since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pMSR:MSMEG_0919:Dendra

Plasmid

#174936

Purpose

C-term fluorescent fusion

Depositor

Keith Derbyshire, Joseph Wade

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_0919

Use

Integrating mycobacterial expression vector attl5…

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Promoter

pmycTetO

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pMSR:MSMEG_5482:Dendra

Plasmid

#167437

Purpose

C-term fluorescent fusion

Depositor

Keith Derbyshire, Joseph Wade

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_5482

Use

Integrating mycobacterial expression vector attl5…

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Promoter

pmycTetO

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pQCXIP mScarlet-MOSPD2 WT

Plasmid

#186472

Purpose

Expression of human MOSPD2 fused to mScarlet in mammalian cells

Depositor

Catherine Tomasetto

Article

Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)

Use

Retroviral

Tags

mScarlet

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pNK5712

Plasmid

#219752

Purpose

pGAP-like vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 under control of GAP promoter, for yeast expression

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

pGAP - nnH3H_v2 - tAOX

Expression

Yeast

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGEM-HSPA5P

Plasmid

#44150

Purpose

a fragment containing 429 bp of the human HSPA5 promoter (-282 to +147)

Depositor

Joan Conaway

Article

Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

Insert

HSPA5 promoter (HSPA5 Human)

Expression

Mammalian

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only
pNK5867

Plasmid

#219751

Purpose

pGAP-like vector encoding hispidin-synthase from Mycena citricolor under control of GAP promoter, for yeast expression

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

pGAP - mcitHispS - tAOX

Expression

Yeast

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pNK1508

Plasmid

#219750

Purpose

pGAP-like vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA under control of GAP promoter, for yeast expression

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

pGAP - npgA - tAOX

Expression

Yeast

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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