We narrowed to 2,493 results for: ERK

1. pEN_TTmiRc2 3XFLAG-MKK4-EE-NLS

   Plasmid

   #87776

   Purpose

   Used as a donor vector containing N-term 3xFLAG to clone into pSLIK-Hygro

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   MKK4 (MAP2K4 Human)
   

   Use

   Tags

   3xFLAG and NLS

   Expression

   Mutation

   Promoter

   Available since

   March 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAG RpH-LAMP1-3xFLAG

   Plasmid

   #163018

   Purpose

   expresses ratiometric sensor of lysosomal lumenal pH

   Depositor

   Massimiliano Stagi

   Article

   Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

   Insert

   RpH-LAMP1-3xFLAG (Lamp1 Mouse)
   

   Use

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. EFNB1-bio-His

   Plasmid

   #51749

   Purpose

   Expresses full-length Ephrin-B1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   EFNB1 (EFNB1 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. 1GFP/RNase H1 WT

   Plasmid

   #174447

   Purpose

   Express GFP-tagged RNase H1 WT in E. coli.

   Depositor

   Karlene Cimprich

   Article

   Crossley et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

   Insert

   RNase H1 (RNASEH1 Human)
   

   Use

   Tags

   His-tag, GFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. piRFP670-N1-GalT

   Plasmid

   #87325

   Purpose

   To express GalT-iRFP670 in mammalian cells, GalT encodes the 1-81 amino acids from β1,4-galactosyltransferase

   Depositor

   Lei Lu

   Article

   Tie et al Mol Biol Cell. 2016 Jan 13. pii: mbc.E15

   Insert

   1-81 amino acids from β1,4-galactosyltransferase (B4GALT1 Human)
   

   Use

   Tags

   iRFP670

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-EFNB2-RBD-Fc (WT)

   Plasmid

   #200978

   Purpose

   Mammalian expression plasmid for soluble EFNB2 (wild type) fused to IgG1 Fc

   Depositor

   Erik Procko

   Article

   Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

   Insert

   EFNB2 (EFNB2 Human)
   

   Use

   Tags

   IgG1 Fc

   Expression

   Mammalian

   Mutation

   Soluble Receptor-Binding Domain

   Promoter

   CMV

   Available since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAG R2pH-LAMP1-3xFLAG

   Plasmid

   #157940

   Purpose

   expresses ratiometric sensor of lysosomal lumenal pH

   Depositor

   Massimiliano Stagi

   Article

   Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

   Insert

   R2pH-LAMP1-3xFLAG (Lamp1 Mouse)
   

   Use

   Tags

   3XFLAG, mCherry, and pHluorin

   Expression

   Mammalian

   Mutation

   Promoter

   Chick beta actin pomoter

   Available since

   Sept. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pF(UG) hSyn LAMP1-msGFP (JV012)

   Plasmid

   #179728

   Purpose

   Lentiviral expression of LAMP1-msGFP for use as a lysosomal marker

   Depositor

   Edwin Chapman

   Article

   Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

   Insert

   LAMP1 (Lamp1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDONOR223 RpH-LAMP1-3xFLAG

   Plasmid

   #163019

   Purpose

   gateway donor vector encoding ratiometric sensor of lysosomal lumenal pH

   Depositor

   Massimiliano Stagi

   Article

   Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

   Insert

   RpH-LAMP1-3xFLAG (Lamp1 Mouse)
   

   Use

   Gateway system

   Tags

   3XFLAG

   Expression

   Mutation

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCEP4-myc-EFNB2 (WT)

    Plasmid

    #200975

    Purpose

    Mammalian expression plasmid for wild type, myc-tagged EFNB2

    Depositor

    Erik Procko

    Article

    Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

    Insert

    EFNB2 (EFNB2 Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET-His6-SUMO-MproC145A

    Plasmid

    #172653

    Purpose

    Bacterial expression of SARS-CoV-2 Mpro C145A inactive mutant fused to SUMO

    Depositor

    Stephen Harrison

    Article

    MacDonald et al ACS Infect Dis. 2021 Sep 10;7(9):2591-25

    Insert

    SARS-CoV-2 Mpro (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    6xHis Tag and SUMO

    Expression

    Bacterial

    Mutation

    Catalytic Cysteine 145 mutated to Alanine

    Promoter

    T7

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. KAT6B-Tol2

    Plasmid

    #113384

    Purpose

    evaluate FOXA1 DIV motif enhancer activity near 50 kb of KAT6B gene

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    KAT6B-enhancer (KAT6B Human)
    

    Use

    Tol2 reporter

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

13. pDONOR223 R2pH-LAMP1-3xFLAG

    Plasmid

    #157941

    Purpose

    gateway donor vector encoding ratiometric sensor of lysosomal lumenal pH

    Depositor

    Massimiliano Stagi

    Article

    Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

    Insert

    R2pH-LAMP1-3xFLAG (Lamp1 Mouse)
    

    Use

    Gateway system

    Tags

    3XFLAG, mCherry, and pHluorin

    Expression

    Mutation

    Promoter

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. KAT6B-luciferase

    Plasmid

    #113353

    Purpose

    evaluate FOXA1 DIV motif enhancer activity near 50 kb of KAT6B gene

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    KAT6B-enhancer (KAT6B Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEX-4T-1 3xHA-MKK4-EE

    Plasmid

    #47577

    Purpose

    Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK4

    Depositor

    Kevin Janes

    Article

    Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

    Insert

    MKK4-EE (MAP2K4 Human)
    

    Use

    Tags

    3xHA and GST

    Expression

    Bacterial

    Mutation

    Lacks the first 36 residues of the RefSeq protein…

    Promoter

    Available since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

16. T7pr-His6-MBP-TEV-dCas9-BirA*

    Plasmid

    #159994

    Purpose

    Bacterial expression of dCas9-BirA*

    Depositor

    Jacob Corn

    Article

    Wang et al Mol Cell. 2020 Jul 16;79(2):221-233.e5.

    Insert

    dCas9-BirA*
    

    Use

    Tags

    6X-His, MBP, 3X-FLAG, NLS

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    T7

    Available since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA4/TO-bio-myc-NES-EGFP

    Plasmid

    #112712

    Purpose

    For tetracycline-inducible expression of bio-myc-NES-EGFP in mammalian cells

    Depositor

    Daniel Schoenberg

    Article

    Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

    Insert

    EGFP
    

    Use

    Tags

    HIV Rev nuclear localization signal (NES), biotin…

    Expression

    Mammalian

    Mutation

    Nucleotide sequence optimized for synthetic gene …

    Promoter

    CMV with Tet repressor binding sites

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. R2pH-LAMP-FRB

    Plasmid

    #225138

    Purpose

    R2pH-LAMP1-labelled lysosomes with FRB

    Depositor

    Massimiliano Stagi

    Article

    Cheetham-Wilkinson et al Sci Adv. 2025 Jan 3;11(1):eadr7325. doi:

    Insert

    Lamp1 (Lamp1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. R2pH-LAMPx3ALFA

    Plasmid

    #225136

    Purpose

    R2pH-LAMP1-labelled lysosomes with 3xLFA

    Depositor

    Massimiliano Stagi

    Article

    Cheetham-Wilkinson et al Sci Adv. 2025 Jan 3;11(1):eadr7325. doi:

    Insert

    Lamp1 (Lamp1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMSCV-FKBP-MAP2K4

    Plasmid

    #221295

    Purpose

    Expression of MAP2K4 (pLxIS) in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    MAP2K4 (pLxIS) (Map2k4 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG-4xFKBP

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge