We narrowed to 10,289 results for: aga

1. pZNF3.1.0-gDNA

   Plasmid

   #132440

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   ZNF3 (ZNF3 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHACK-GAL4>GAL80

   Plasmid

   #104874

   Purpose

   HACK Donor plasmids for converting GAL4 lines to GAL80 lines using HACK method.

   Depositor

   Christopher Potter

   Insert

   T2A-GAL80
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTRY4_ Pi21

   Plasmid

   #126904

   Purpose

   Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.

   Depositor

   Sophien Kamoun

   Insert

   Wheat_live_Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. CRISPR plasmid

   Plasmid

   #236553

   Purpose

   Encodes Cas9 and two guideRNAs, one to cut the genome downstream of HSPA5 (BiP/GRP78) and one to linearize the DONOR plasmid.

   Depositor

   Pablo Rivera-Fuentes

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCas9(BB)-2A-Blast_HP1beta_gRNA

   Plasmid

   #127908

   Purpose

   WT Cas9 Vector with Blasticidin Selection Marker targeting the 5' end of the human HP1beta gene

   Depositor

   Mark Groudine

   Insert

   gRNA for Human 5' HP1beta
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pICSL4723_ Pi21

   Plasmid

   #126892

   Purpose

   Genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.

   Depositor

   Sophien Kamoun

   Insert

   Wheat_live_Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTRY4_ WRKY28_2

   Plasmid

   #126900

   Purpose

   Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.

   Depositor

   Sophien Kamoun

   Insert

   Wheat_live_Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJK138

   Plasmid

   #73314

   Purpose

   Pas2p peroxisomal membrane targeting sequence

   Depositor

   T. J. Kappock

   Insert

   Pas2p (peroxisomal membrane targeting region)
   

   Use

   Tags

   His6 and c-myc

   Expression

   Yeast

   Mutation

   encodes residues 1-42

   Promoter

   AOX1

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. bU6-sgSik1_1st-mU6-sgSik2-hU6-sgSik3_1st

   Plasmid

   #177232

   Purpose

   Expresses Sik1(bU6), Sik2 (mU6) and Sik3 (hU6) gRNAs and Cre-recombinase

   Depositor

   Monte Winslow

   Insert

   sgSik1_1st/sgSik2/sgSik3_1st
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   bU6/mU6/hU6

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-GR-GECO1.2

    Plasmid

    #42187

    Depositor

    Robert Campbell

    Article

    Hoi et al J Am Chem Soc. 2012 Dec 26.

    Insert

    GR-GECO1.2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pLKO.1-TRC.mKO2_shHuR CDS

    Plasmid

    #110426

    Purpose

    TRCN0000276129 (Target CGAGCTCAGAGGTGATCAAAG), silence human ELAVL1 (HuR) gene and express monomeric Kusabira-Orange2.

    Depositor

    Sandra Martha Gomes Dias

    Insert

    ELAVL1 HuR
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (RNA Pol III)

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-GR-GECO1.1

    Plasmid

    #42186

    Depositor

    Robert Campbell

    Article

    Hoi et al J Am Chem Soc. 2012 Dec 26.

    Insert

    GR-GECO1.1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

13. bU6-sgNeo1-mU6-sgNuak1-hU6-sgNuak2

    Plasmid

    #177234

    Purpose

    Expresses Neomycin (bU6), Nuak1 (mU6) and Nuak2(hU6) gRNAs and Cre-recombinase

    Depositor

    Monte Winslow

    Insert

    sgNeo1/sgNuak1/sgNuak2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    bU6/mU6/hU6

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pML107-KAE1

    Plasmid

    #232891

    Purpose

    Plasmid expressing Cas9 and gRNA GATGACAACTGAATGCAGAG which targets the KAE1 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    KAE1 gRNA (KAE1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pML107-TLG1

    Plasmid

    #232897

    Purpose

    Plasmid expressing Cas9 and gRNA GATGAAAACGAAGACGTGAG which targets the TLG1 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    TLG1 gRNA (TLG1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pML104-LEU2-2

    Plasmid

    #232883

    Purpose

    Plasmid expressing Cas9 and gRNA GGTAGTGTTAGACCTGAACA which targets the LEU2 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    LEU2 gRNA (LEU2 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pML107-INO80

    Plasmid

    #232890

    Purpose

    Plasmid expressing Cas9 and gRNA GGAAGTAGAATCATTCGTAG which targets the INO80 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    INO80 gRNA (INO80 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNSU299_PDR12

    Plasmid

    #166093

    Purpose

    Plasmid for constituive spCas9 and tet-inducible PDR12 targeting sgRNA expression for double stranded break formation in yeast

    Depositor

    Rebecca Burgess

    Insert

    PDR12 (PDR12 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Tet-inducible

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJK139

    Plasmid

    #73315

    Purpose

    Pas2p peroxisomal membrane biogenesis protein

    Depositor

    T. J. Kappock

    Insert

    Pas2p peroxisomal membrane targeting protein
    

    Use

    Tags

    His6 and c-myc

    Expression

    Yeast

    Mutation

    Encodes a glycine-204 to serine mutation

    Promoter

    AOX1

    Available since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBCPB+

    Plasmid

    #18940

    Depositor

    Michele Calos

    Article

    Groth et al Proc Natl Acad Sci U S A. 2000 May 23. 9

    Insert

    attP, lacZ, attB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 13, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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