We narrowed to 2,459 results for: GEM

1. pLenti-Synapsin-pHoenix-WPRE

   Plasmid

   #70111

   Purpose

   lentiviral expression of pHoenix (Synaptophysin-pHluorin-Arch3-mKate2) driven by human Synapsin I promoter for optogenetic acidification of synaptic vesicles

   Depositor

   Christian Rosenmund

   Article

   Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

   Insert

   pHoenix (Syp Rat, Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria)
   

   Use

   Lentiviral

   Tags

   Arch3, H+/K+ ATPase beta-subunit, mKate2, and pHl…

   Expression

   Mutation

   Promoter

   Synapsin

   Available since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. mouse E-cadherin GFP (423)

   Plasmid

   #67937

   Purpose

   Mammalian expression of E-cadherin-GFP

   Depositor

   Alpha Yap

   Article

   Truffi et al J Cell Sci. 2014 Jun 1;127(Pt 11):2420-3

   Insert

   E-cadherin (Cdh1 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. TetOn-CLIMP63-mCherry-eGFP

   Plasmid

   #139871

   Purpose

   Dox-inducible lentiviral expression of human CLIMP63

   Depositor

   Jacob Corn

   Article

   Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

   Insert

   CLIMP63 (CKAP4 Human)
   

   Use

   Lentiviral

   Tags

   mCherry-eGFP

   Expression

   Mutation

   Promoter

   Available since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+)-HLA-Flag-natT1R3

   Plasmid

   #113950

   Purpose

   mammalian expression plasmid for FLAG-tagged human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

   Depositor

   Erik Procko

   Article

   Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

   Insert

   T1R3 (TAS1R3 Human)
   

   Use

   Tags

   FLAG and Signal/leader sequence from HLA class I …

   Expression

   Mammalian

   Mutation

   residues 21-852

   Promoter

   Available since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFBD-Akt13C(S473D)

   Plasmid

   #86577

   Purpose

   Expresses cleavable human full-length Akt1(S473D) phosphomimetic

   Depositor

   Thomas Leonard, Ivan Yudushkin

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1 (AKT1 Human)
   

   Use

   Tags

   10xHis

   Expression

   Insect

   Mutation

   3C cleavage site inserted at pos. 130; S473D phos…

   Promoter

   Available since

   April 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKLV-EF1aGFP2AKAT7(E508Q)-W

   Plasmid

   #159293

   Purpose

   Lentiviral vector expressing GFP and KAT7 (gRNA-resistant, E508Q mutant) driven by human EF1a promoter

   Depositor

   Kosuke Yusa

   Article

   Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

   Insert

   KAT7 E508Q
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   CRISPR sites mutated (gRNA ID. 5 and A10) and E50…

   Promoter

   human EF1a promoter

   Available since

   Dec. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CamkII-bPac(WT)-mCherry-minWPRE.sbd

   Plasmid

   #118278

   Purpose

   Expresses bPAC(WT)-mCherry under a minimal CamKII Promoter

   Depositor

   Dietmar Schmitz

   Article

   Bernal Sierra et al Nat Commun. 2018 Nov 5;9(1):4611. doi: 1

   Insert

   bPAC(WT)
   

   Use

   AAV

   Tags

   mCherry and myc

   Expression

   Mutation

   Promoter

   CamKII

   Available since

   June 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. ESR1-luciferase

   Plasmid

   #113351

   Purpose

   evaluate FOXA1 DIV motif enhancer activity near 50 kb of ESR1 gene

   Depositor

   Ralf Jauch

   Article

   Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

   Insert

   ESR1-enhancer (ESR1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCEP4-HA-Flag-natT1R2 FS1

   Plasmid

   #113955

   Purpose

   mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

   Depositor

   Erik Procko

   Article

   Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

   Insert

   T1R2 (TAS1R2 Human)
   

   Use

   Tags

   FLAG and Signal/leader sequence from influenza he…

   Expression

   Mammalian

   Mutation

   delete N-terminal 21 residues, one base pair dele…

   Promoter

   cmv

   Available since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLIB-GST-3C-CDK1

    Plasmid

    #177011

    Purpose

    Generate baculovirus for insect cell expression of CDK1 with N-terminal GST-3C (PreScission)

    Depositor

    Andrea Musacchio

    Article

    Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

    Insert

    CDK1 (CDK1 Human)
    

    Use

    Tags

    GST-3C

    Expression

    Insect

    Mutation

    codon-optimised for insect cell expression

    Promoter

    Available since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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