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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 4,184 results for: 256

Showing: 2481 - 2500 of 4184 results

  2. pJK215

    Plasmid
    #72427
    Purpose
    Acetobacter aceti 1023 gDNA
    Depositor
    T. J. Kappock
    Inserts
    succinyl-diaminopimelate desuccinylase
    2,3,4,5-tetrahydropyridine-2,6-carboxylate N-succinyltransferase
    acetylglutamate kinase
    ribosome biogenesis GTP-binding protein YsxC
    insertase
    ribonuclease P
    50S ribosomal protein L34
    hypothetical protein
    glyoxylase I
    hypothetical protein
    UDP-N-acetylglucosamine acyltransferase
    3-hydroxyacyl-ACP dehydratase
    UDP-3-O-(3-hydroxymyristoyl) glucosamine N-acyltransferase
    Use
    Phage lambda cloning vector with genomic dna inse…
    Tags
    Expression
    Mutation
    missing C-terminal domain residues 277-391(ter) a…
    Promoter
    n/a
    Available since
    March 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    72427-plasmid-map-sequence-id-137744

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  3. pDule2-3-nitroTyrosine (A7)

    Plasmid
    #174079
    Purpose
    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Beyer et al J Mol Biol. 2020 Jul 24;432(16):4690-470
    Insert
    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y32H H70T D158H I159A L162R
    Promoter
    GlnRS (constitutive)
    Available since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    174079-plasmid-map-sequence-id-341130

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  4. CA-RIT-NFAT1 (IRES-GFP)

    Plasmid
    #85181
    Purpose
    Constitutively active NFAT1, mutated to interfere selectively with the NFAT:AP-1 interaction. Co-expresses EGFP for selection
    Depositor
    Anjana Rao
    Article
    Martinez et al Immunity. 2015 Feb 6. pii: S1074-7613(15
    Insert
    CA-RIT-NFAT1 (Nfatc2 Mouse)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Amino acids 1-3 removed; CA: PASSGSSASF mutated t…
    Promoter
    Available since
    April 12, 2017
    Availability
    Academic Institutions and Nonprofits only

  8. pD649-HAsp-NEGR1-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156728
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    NEGR1 (NEGR1 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156728-plasmid-map-sequence-id-434531

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  9. pD649-HAsp-KIR2DS1-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156675
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    KIR2DS1 (KIR2DS1 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    156675-plasmid-map-sequence-id-383691

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  10. pcDNA5/FRT/TO-GAlphai1-RLuc8

    Plasmid
    #140973
    Purpose
    Encodes a G alpha subunit (GNAl1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphai1-RLuc8 (GNAI1 Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140973-plasmid-map-sequence-id-300655

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  11. pD649-HAsp-SCN1B-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156534
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    SCN1B (SCN1B Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    Aug. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    156534-plasmid-map-sequence-id-368609

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  12. TFORF0314

    Plasmid
    #141579
    Purpose
    Lentiviral vector for overexpressing the CREM transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CREM (CREM Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141579-plasmid-map-sequence-id-400422

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  14. pD649-HAsp-HLA-B-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156835
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    HLA-B (HLA-B Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156835-plasmid-map-sequence-id-475924

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  16. pD649-HAsp-TNFRSF12A-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156487
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    TNFRSF12A (TNFRSF12A Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    Aug. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    156487-plasmid-map-sequence-id-368388

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  17. pD649-HAsp-PTPRF-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #157036
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    PTPRF (PTPRF Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    157036-plasmid-map-sequence-id-401022

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  18. pD649-HAsp-OPCML-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156733
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    OPCML (OPCML Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156733-plasmid-map-sequence-id-434532

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Showing: 2481 - 2500 of 4184 results