We narrowed to 8,310 results for: Dos

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pCPH2A.X_S139A-GS3

Plasmid

#204749

Purpose

Expression of Cas9 and human H2AX with the S139A mutation

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2AX S139A (H2AX Human)

Expression

Mammalian

Mutation

H2AX with the S139A mutation

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-EGFP-C-IFT81

Plasmid

#218732

Purpose

Expresses N-terminally EGFP-tagged IFT81 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Ishida et al Mol Biol Cell. 2022 Aug 1;33(9):ar83. do

Insert

IFT81 (IFT81 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB405-TIC21-sGFP

Plasmid

#213865

Purpose

Binary vector for the expression of sGFP flanked with an N-terminal chloroplas inner envelope membrane transit peptide in plants .

Depositor

Yutaka Kodama

Article

Ichikawa et al Plant Direct. 2022 Nov 15;6(11):e462. do

Insert

chloroplast inner envelope membrane transit peptide (TIC21 Mustard Weed)

Expression

Bacterial and Plant

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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EC67841

Plasmid

#209512

Purpose

Cloning in complete guide expression cassettes, with Cas12a (D156R)

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsDCP2-E148Q_U

Plasmid

#147643

Purpose

Mammalian Expression of HsDCP2-E148Q

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsDCP2-E148Q (DCP2 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-mCherry-p115(S240A)

Plasmid

#181997

Purpose

Mammalian expression vector encoding S240A-mutated p115 RhoGEF tagged with mCherry.

Depositor

Jin Zhang

Article

Zhang et al PLoS Biol. 2020 Sep 3;18(9):e3000866. do

Insert

mCherry-p115(S240A) (ARHGEF1 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Serine 240 changed to alanine in p115.

Promoter

CMV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAVS1-TLR6

Plasmid

#118997

Purpose

Targeting vector for human AAVS1 with TLR6 reporter insert

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

AAVS1 (PPP1R12C, AAVS1 Human)

Expression

Mammalian

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pUC-DEST-cjBLIMP1-Venus-HygTK

Plasmid

#141028

Purpose

Reporter integration

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

mVenus

Expression

Mammalian

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-L1-PGK-PuroTK-L2

Plasmid

#141005

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

PuroTK

Expression

Mammalian

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pUC-DEST-R3R4(R)

Plasmid

#141010

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Expression

Mammalian

Available Since

July 6, 2020

Availability

Academic Institutions and Nonprofits only

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p3E tdTomato

Plasmid

#135210

Purpose

Red fluorescent tag

Depositor

David Tobin

Article

Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

Insert

tdTomato

Use

Synthetic Biology

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mCherry-CyrCR1.mutant

Plasmid

#128746

Purpose

Expresses mCherry in mammalian cells. ~200 nt of Cyrano with 5 point mutations in miR-7 site inserted in mCherry 3'UTR.

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

Cyrano conserved region (CR1) with mutant miR-7 site (OIP5-AS1 Human)

Expression

Mammalian

Mutation

5 point mutations disrupting the miR-7 site

Promoter

CMV

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pTORGm43GW

Plasmid

#112902

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin.

Depositor

Sebastian Schornack

Article

Evangelisti et al BMC Microbiol. 2019 Nov 27;19(1):265. do

Type

Empty backbone

Use

Oomycete expression

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pWS1806

Plasmid

#123047

Purpose

Melatonin GPCR

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

MTNR1A [3]

Expression

Yeast

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pRRLSIN.cPPT.PGK.NeuroD2.WPRE

Plasmid

#122054

Purpose

Lentiviral expression of NeuroD2

Depositor

Stephen Tapscott

Article

Fong et al Cell Rep. 2015 Mar 31;10(12):1937-46. do

Insert

NeuroD2 (Neurod2 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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Kv7.4 (1-645, Δ368-523)/HA tag/pcDNA3.1

Plasmid

#111459

Purpose

Electrophysiology in HEK cell

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Tags

Ha Tag

Expression

Mammalian

Mutation

Kv7.4 (1-645, Δ368-523), C643A

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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LZF80: Cre-on CAG-somCheRiff-CFP (DIO)

Plasmid

#104115

Purpose

synOptopatch construct

Depositor

Adam Cohen

Article

Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

Insert

somCheRiff-CFP

Use

Lentiviral

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only
pJOG641

Plasmid

#105427

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

GAL4-VP16-GR (transcription factor: inducible expression; dexamethasone)

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pTEI036

Plasmid

#105350

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

LexA operator & 35S min promoter

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-ChBP

Plasmid

#108892

Purpose

Multisite gateway vector for 3' tagging with a nanobody against mCherry

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

ChBP

Use

Gateway Cloning

Available Since

June 23, 2018

Availability

Academic Institutions and Nonprofits only

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