We narrowed to 3,688 results for: MAK;

1. pAAV-Ef1a-Coff/Fon-mCherry

   Plasmid

   #137134

   Purpose

   Intersectional viral expression of mCherry in cells expressing Flp AND NOT Cre

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Coff/Fon-mCherry
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   n/a

   Promoter

   EF1a

   Available since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCXLE-mp53DD

   Plasmid

   #41859

   Purpose

   Non-integrating (episomal) expression of mouse p53DD - p53 carboxy-terminal dominant-negative fragment

   Depositor

   Shinya Yamanaka

   Article

   Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

   Insert

   Trp 53 (Trp53 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Δ40-903

   Promoter

   CAG

   Available since

   July 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn Coff/Fon hChR2(H134R)-EYFP

   Plasmid

   #55648

   Purpose

   Cre-off/Flp-on ChR2-EYFP under the Synapsin promoter

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Insert

   ChR2(H134R)-EYFP
   

   Use

   AAV; Cre off/flp on chr2-eyfp

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHLS-EF1a-FKBP12-Gag(HIV)

   Plasmid

   #138476

   Purpose

   Expresses FKBP12 fused Gag for packaging FRB-SpCas9 into NanoMEDIC particle.

   Depositor

   Akitsu Hotta

   Article

   Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

   Insert

   HIV Gag
   

   Use

   Tags

   FRBP12 and Lynn Myristolation site

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-nEF-Coff/Fon-iC++-EYFP

   Plasmid

   #137157

   Purpose

   Intersectional viral expression of iC++-EYFP in cells expressing Flp AND NOT Cre

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Coff/Fon-iC++-EYFP
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   n/a

   Promoter

   nEF

   Available since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-nEF-Coff/Fon DREADD Gi-mCherry

   Plasmid

   #177669

   Purpose

   Expresses DREADD hM4D (Gi) only in Flp expressing cells and not in Cre expressing cells

   Depositor

   Karl Deisseroth

   Article

   Knoedler et al Cell. 2022 Feb 17;185(4):654-671.e22. do

   Insert

   DREADD Gi-mCherry
   

   Use

   AAV, Cre/Lox, and Mouse Targeting ; Intrsect

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   nEF

   Available since

   March 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-Hygro-EF1A-EGFP-PARP1

   Plasmid

   #176146

   Purpose

   EGFP fused to the N-terminus of PARP1 & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   Poly(ADP-Ribose) Polymerase 1 (PARP1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFUSEss-CHIg-mG3_M18

   Plasmid

   #82356

   Purpose

   Expression plasmid coding for heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18.

   Depositor

   Joanna Bereta

   Article

   Klaus et al Sci Rep. 2016 Aug 3;6:30938. doi: 10.103

   Insert

   mouse immunoglobulin heavy chain IgG3 isotype (Ighg3 Mouse)
   

   Use

   Tags

   none

   Expression

   Mammalian

   Mutation

   none

   Promoter

   hEF1-HTLV prom

   Available since

   Sept. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. PL-5LTR-RGR(DMD​#​1​)-AmCyan-A

   Plasmid

   #138482

   Purpose

   Expresses sgRNA targeting human DMD (Dystrophin) for packaging into NanoMEDIC particle.

   Depositor

   Akitsu Hotta

   Article

   Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

   Insert

   Ribozyme-flanked gRNA and AmCyan
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. FUW-TetO-Gata6

    Plasmid

    #128827

    Purpose

    dox-dependent expression of mouse Gata6

    Depositor

    Yossi Buganim

    Article

    Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

    Insert

    Gata6 (Gata6 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. mVenus-proα1(I)

    Plasmid

    #119845

    Purpose

    Expresses mouse Type I procollagen α1 chain (Col1a1) with mVenus replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

    Depositor

    Sergey Leikin

    Article

    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

    Insert

    Type I procollagen α1 chain (Col1a1 Mouse)
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Col1a1 exons 2-3 replaced by fluorescent tag

    Promoter

    CMV

    Available since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR-GAL4-UAS-huCD25- IRES-mCherry-PGK-BFP

    Plasmid

    #223211

    Purpose

    Lentiviral vector - mCherry reporter and huCD25 for Gal4DBDVP64 synNotch receptors with a constitutive BFP

    Depositor

    Wendell Lim

    Article

    Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

    Insert

    CD25 (IL2RA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLIB_GST-TEV-SRC(WT)

    Plasmid

    #223742

    Purpose

    Expression of recombinant protein for purification, codon optimized for Insect cells

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    Src Kinase (SRC Human)
    

    Use

    Tags

    GST-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCas-pML-1B_6xHis-Cas12m

    Plasmid

    #192282

    Purpose

    MmCas12m fused to His-tag for protein purification

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Insert

    MmCas12m
    

    Use

    CRISPR

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5-FRT-TO-EGFR*-CUbo

    Plasmid

    #212821

    Purpose

    Constitutive or doxycycline-inducible expression of EGFR*-CUbo in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    EGFR* (EGFR Budding Yeast, Human)
    

    Use

    Tags

    CUbo

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGS-FuG-E

    Plasmid

    #67509

    Purpose

    This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. If you use this plasmid, you can make type E envelope coating virus particle with NeuRet.

    Depositor

    Kazuto Kobayashi

    Article

    Kato et al J Neurosci Methods. 2014 Apr 30;227:151-

    Insert

    Fusion Glycoprotein type E
    

    Use

    Lentiviral

    Tags

    Fusion protein of Vesicular stomatitis Indiana vi…

    Expression

    Mutation

    Promoter

    CAGGS

    Available since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-nEF Con/Fon hChR2(H134R)-EYFP

    Plasmid

    #55644

    Purpose

    Cre-on/Flp-on ChR2-EYFP under the short Ef1a promoter

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV; Cre on/flp on chr2-eyfp

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    nEF

    Available since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pETDuet-1_6xHis-TEV-mCh-NDP52

    Plasmid

    #187829

    Purpose

    Plasmid for the expression and purification of 6xHis-TEV-mCh-NDP52 in E. coli Rosetta pLysS. Internal Reference: SMC1148

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    NDP52 (CALCOCO2 Human)
    

    Use

    Tags

    6xHisTag, TEV, and mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCRISPR-RFP entry

    Plasmid

    #192278

    Purpose

    Entry vector for cloning MmCas12m spacers. RFP flanked by MmCas12m CRISPR repeats can be digested out with BbsI.

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Insert

    RFP flanked by type V-M CRISPR repeat sequences
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23119

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV-hSyn-ASAP4b-Kv-WPRE

    Plasmid

    #201030

    Purpose

    Expresses somatically enriched ASAP4b in neurons, can be used to package AAV

    Depositor

    Michael Lin

    Article

    Evans et al Nat Methods. 2023 Jul;20(7):1104-1113. d

    Insert

    ASAP4b-Kv
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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