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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 42,599 results for: cha

Showing: 2481 - 2500 of 42599 results

  1. pBY011-AMN1ko

    Plasmid
    #183099
    Purpose
    S. cerevisiae Sigma1278b AMN1 gene knock out plasmid with KanMX selection marker.
    Depositor
    Gabor Balazsi
    Article
    Guinn et al Commun Biol. 2022 Mar 31;5(1):292. doi:
    Insert
    AMN1 (left homology arm) - KanMX - AMN1 (right homology arm) (AMN1 Budding Yeast)
    Use
    Tags
    KanMX
    Expression
    Bacterial and Yeast
    Mutation
    only includes external sequences (504 bp each) as…
    Promoter
    URA3, TEF1
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183099-plasmid-map-sequence-id-361230

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  2. pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (wild type)

    Plasmid
    #153439
    Purpose
    E. coli expression of Rc. XDocB wild-type construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.
    Depositor
    Michael Nash
    Article
    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:
    Insert
    Rc.XDocB
    Use
    Tags
    3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    153439-plasmid-map-sequence-id-306439

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  8. pCAG-mEGFP-CaMKIIa (T286D/T305A/T306A)

    Plasmid
    #127392
    Purpose
    Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286D/T305A/T306A)
    Depositor
    Ryohei Yasuda
    Article
    Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:
    Insert
    calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    changed Threonine 286 to Aspartic acid and Threon…
    Promoter
    pCAG
    Available since
    July 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    127392-plasmid-map-sequence-id-250433

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  9. pET28a-ybbr-HIS-ELP-ddFLN4-I27-XMod-Doc (wild type)

    Plasmid
    #153442
    Purpose
    E. coli expression of Rc. XDocB wild-type construct containing ybbr tag, ELP linker and ddFLN4 and I27 fingerprint domains. This construct was designed for AFM measurements.
    Depositor
    Michael Nash
    Article
    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:
    Insert
    Rc.XDocB
    Use
    Tags
    3xELP linker, 6xHis, Titin I27, ddFLN4 fingerprin…
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    Sept. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    153442-plasmid-map-sequence-id-310821

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  10. pAC8_PH-GST-Nter-GWs-Lox (VE5586)

    Plasmid
    #163766
    Purpose
    Transfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette with an N-ter GST tag under the pH promoter.
    Depositor
    Arnaud Poterszman
    Article
    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.
    Insert
    N-terminal GST tag
    Use
    Tags
    GST Tag
    Expression
    Insect
    Mutation
    Promoter
    PH
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    163766-plasmid-map-sequence-id-379000

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  11. pAC8_PH-6His-Nter-GWs-Lox (VE5587)

    Plasmid
    #163768
    Purpose
    Transfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette with an N-ter 6 His tag under the pH promoter.
    Depositor
    Arnaud Poterszman
    Article
    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.
    Insert
    N-terminal 6His tag
    Use
    Tags
    6 His Tag
    Expression
    Insect
    Mutation
    Promoter
    PH
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    163768-plasmid-map-sequence-id-379027

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  12. pAC8_MF-PH-10His-Cter (VE5631)

    Plasmid
    #161802
    Purpose
    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter10His tag under the p10 promoter.
    Depositor
    Arnaud Poterszman
    Article
    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.
    Insert
    C-terminal 10 His tag
    Use
    Tags
    10 His
    Expression
    Insect
    Mutation
    Promoter
    p10
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    161802-plasmid-map-sequence-id-324122

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  13. pAC8_MF-P10-mCherry-pH-3C-TwinStrep (VE5621)

    Plasmid
    #139770
    Purpose
    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with a C-ter TwinStrep tag under the PH promoter.
    Depositor
    Arnaud Poterszman
    Article
    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.
    Insert
    C-terminal TwinStrep tag
    Use
    Tags
    3C - TwinStrep tag
    Expression
    Mutation
    Promoter
    PH
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    139770-plasmid-map-sequence-id-324179

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  14. pAC8_MF-p10-10His-Cter (VE5742)

    Plasmid
    #139776
    Purpose
    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter10His tag under the pH promoter.
    Depositor
    Arnaud Poterszman
    Article
    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.
    Insert
    C-terminal 10His tag
    Use
    Tags
    10 His
    Expression
    Insect
    Mutation
    Promoter
    PH
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    139776-plasmid-map-sequence-id-324157

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  15. pAC8_MF-p10-Flag-Cter(VE5738)

    Plasmid
    #161798
    Purpose
    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter Flag tag under the p10 promoter.
    Depositor
    Arnaud Poterszman
    Article
    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.
    Insert
    C-terminal Flag tag
    Use
    Tags
    Flag
    Expression
    Insect
    Mutation
    Promoter
    p10
    Available since
    May 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    161798-plasmid-map-sequence-id-324175

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  16. pAC8_PH-10His-Nter-GWs-Lox (VE5588)

    Plasmid
    #161806
    Purpose
    Transfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette with an N-ter 10 His tag under the pH promoter.
    Depositor
    Arnaud Poterszman
    Article
    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.
    Insert
    N-terminal 10His tag
    Use
    Tags
    10 His
    Expression
    Insect
    Mutation
    Promoter
    PH
    Available since
    May 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    161806-plasmid-map-sequence-id-324125

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  17. pAC8_MF-p10-mCh PH-mCh (VE5625)

    Plasmid
    #139769
    Purpose
    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an mCherry cDNA under each promoter.
    Depositor
    Arnaud Poterszman
    Article
    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.
    Insert
    mCherry fluorescent protein
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    PH or p10
    Available since
    May 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    139769-plasmid-map-sequence-id-324116

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  20. CLC-EGFP206(5)

    Plasmid
    #193573
    Purpose
    Expresses human Clathrin Light Chain B tagged with mEGFP at amino acid position 206 with a rigid alpha helix of 5 amino acids in mammalian cells.
    Depositor
    Justin Taraska
    Article
    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1
    Insert
    Clathrin light chain B (CLTB Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Deleted amino acids 207-211
    Promoter
    CMV
    Available since
    Feb. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    193573-plasmid-map-sequence-id-380171

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Showing: 2481 - 2500 of 42599 results