We narrowed to 44,083 results for: cha

1. pFA6a-TRP1-PGAL1-GFP

   Plasmid

   #41615

   Depositor

   John Pringle

   Article

   Longtine et al Yeast. 1998 Jul;14(10):953-61.

   Insert

   GAL1 promoter (GAL1 Budding Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   GFP(S65T), TRP1 reverse orientation, and yeast AD…

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 11, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAV0756

   Plasmid

   #133508

   Purpose

   Expression of 3mCherry tagged CRIB domain to monitor active Cdc42

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pHis5(StuI)-p(act1)-CRIB(gic2aa2-181)-3mCherry-bsdMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAV0764

   Plasmid

   #133520

   Purpose

   Expression of mCherry targeted to the endoplasmic reticulum with a signal sequence and a retention signal (AHDL)

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pLys3(BstZ17I)-p(BiP)-SignalSequenceBiP-mCherry-AHDL-bsdMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAV0773

   Plasmid

   #133519

   Purpose

   Expression of sfGFP targeted to the endoplasmic reticulum with a signal sequence and a retention signal (AHDL)

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pLys3(BstZ17I)-p(BiP)-SignalSequenceBiP-sfGFP-AHDL-bsdMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAV0835

   Plasmid

   #133512

   Purpose

   Expression of mCherry tagged CHD domain to monitor actin cytoskeleton

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pUra4(AfeI)-p(act1)-mCherry-CHDRng2-terminator(ScCYC1)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAV0816

   Plasmid

   #133510

   Purpose

   Expression of mTagBFP2 tagged CRIB domain to monitor active Cdc42

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pAde6(PmeI)-p(act1)-CRIB(gic2aa2-181)-mTagBFP2-terminator(ScADH1)-bsdMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p-mCherry-N1-slowChloC

   Plasmid

   #85462

   Purpose

   Historical plasmid. For inhibition of neuronal activity, #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)

   Depositor

   Peter Hegemann

   Article

   Wietek et al Science. 2014 Mar 27.

   Insert

   slowChloC
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   E90R, T159C, D156N

   Promoter

   CMV (+enhancer)

   Available Since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFA6a-TRP1-PGAL1-GST

   Plasmid

   #41612

   Depositor

   John Pringle

   Article

   Longtine et al Yeast. 1998 Jul;14(10):953-61.

   Insert

   GAL1 promoter (GAL1 Budding Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   GST, TRP1 reverse orientation, and yeast ADH1 ter…

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 11, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMH-HT_ygiF-FL

   Plasmid

   #127795

   Purpose

   Expresses full-length ygiF in E. coli cells

   Depositor

   Michael Hothorn

   Article

   Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

   Insert

   ygiF (ygiF Escherichia coli)
   

   Use

   Tags

   6xHis tag + TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. VV228: sdChR(C138S E154A)-TS in fck

    Plasmid

    #58523

    Purpose

    Expresses sdChR(C138S E154A) in mammalian cells

    Depositor

    Adam Cohen

    Article

    Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

    Insert

    sdChR(C138S, E154A)-TS
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    changed Cysteine 138 to Serine; changed Glutamate…

    Promoter

    a-CamKII

    Available Since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. myc-SpEAF pBacPAK8

    Plasmid

    #15320

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

    Insert

    ELL associated factor (eaf1 Fission Yeast)
    

    Use

    Baculovirus construction

    Tags

    Myc

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pJET-TtrpC

    Plasmid

    #213467

    Purpose

    Vector containing the TrpC terminator for Golden Gate cloning in the Fg vector

    Depositor

    Kim Hammond-Kosack

    Article

    Darino et al Fungal Biol Biotechnol. 2024 Feb 26;11(1

    Insert

    Terminator of tryptophan synthase of Aspergillus nidulans
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJET-PgpdA

    Plasmid

    #213466

    Purpose

    Vector containing the gpdA promoter for Golden Gate cloning in the Fg vector

    Depositor

    Kim Hammond-Kosack

    Article

    Darino et al Fungal Biol Biotechnol. 2024 Feb 26;11(1

    Insert

    Promoter of the glyceraldehyde-3-phosphate dehydrogenase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJET-PtrpC

    Plasmid

    #213465

    Purpose

    Vector containing the TrpC promoter for Golden Gate cloning in the Fg vector

    Depositor

    Kim Hammond-Kosack

    Article

    Darino et al Fungal Biol Biotechnol. 2024 Feb 26;11(1

    Insert

    Promoter of tryptophan synthase of Aspergillus nidulans
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. KBB556

    Plasmid

    #185108

    Purpose

    1st 200 amino acids (N-term + transmembrane domain) of Pom152 fused to HA and GFP with S158A, F159A, F160A

    Depositor

    Ken Belanger

    Article

    Brown et al Biol Open. 2021 Oct 15;10(10). pii: 2722

    Insert

    POM152
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    1st 200 amino acids (N-term + transmembrane domai…

    Promoter

    Available Since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. KBB553

    Plasmid

    #185107

    Purpose

    1st 200 amino acids (N-term + transmembrane domain) of Pom152 fused to HA and GFP with Y180L, Q182L

    Depositor

    Ken Belanger

    Article

    Brown et al Biol Open. 2021 Oct 15;10(10). pii: 2722

    Insert

    POM152
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    1st 200 amino acids (N-term + transmembrane domai…

    Promoter

    Available Since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-Avi-Coh (E154C)-HIS

    Plasmid

    #153447

    Purpose

    E. coli expression of Rc. CohE construct with E154C mutation.

    Depositor

    Michael Nash

    Article

    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

    Insert

    Rc.CohE
    

    Use

    Tags

    6xHis and Avi-tag

    Expression

    Bacterial

    Mutation

    E154C mutation on CohE

    Promoter

    T7 promoter

    Available Since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAV0747

    Plasmid

    #133481

    Purpose

    Expression in fission yeast from promoter ppom1 , integrated at ura4 locus

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pUra4(AfeI)-p(pom1)-sfGFP-terminator(ScCYC1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAV0748

    Plasmid

    #133482

    Purpose

    Expression in fission yeast from promoter ppak1 , integrated at ura4 locus

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pUra4(AfeI)-p(pak1)-sfGFP-terminator(ScCYC1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAV0605

    Plasmid

    #133503

    Purpose

    Expression of sfGFP targeted to the plasma membrane with an amphipathic helix of RitC

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pUra4(AfeI)-p(act1)-sfGFP-RitC-terminator(tdh1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge