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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 42,612 results for: cha

Showing: 2501 - 2520 of 42612 results

  1. CLC-EGFP206(6)

    Plasmid
    #193574
    Purpose
    Expresses human Clathrin Light Chain B tagged with mEGFP at amino acid position 206 with a rigid alpha helix of 6 amino acids in mammalian cells.
    Depositor
    Justin Taraska
    Article
    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1
    Insert
    Clathrin light chain B (CLTB Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Deleted amino acids 207-211
    Promoter
    CMV
    Available since
    Feb. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    193574-plasmid-map-sequence-id-380173

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  2. CLC-EGFP206(7)

    Plasmid
    #193575
    Purpose
    Expresses human Clathrin Light Chain B tagged with mEGFP at amino acid position 206 with a rigid alpha helix of 7 amino acids in mammalian cells.
    Depositor
    Justin Taraska
    Article
    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1
    Insert
    Clathrin light chain B (CLTB Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Deleted amino acids 207-211
    Promoter
    CMV
    Available since
    Feb. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    193575-plasmid-map-sequence-id-383667

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  3. CLC-EGFP206(flex5)

    Plasmid
    #193576
    Purpose
    Expresses human Clathrin Light Chain B tagged with mEGFP at amino acid position 206 with a flexible linker of 5 amino acids in mammalian cells.
    Depositor
    Justin Taraska
    Article
    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1
    Insert
    Clathrin light chain B (CLTB Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Deleted amino acids 207-211
    Promoter
    CMV
    Available since
    Feb. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    193576-plasmid-map-sequence-id-380174

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  4. CLC-EGFP206(flex6)

    Plasmid
    #193577
    Purpose
    Expresses human Clathrin Light Chain B tagged with mEGFP at amino acid position 206 with a flexible linker of 6 amino acids in mammalian cells.
    Depositor
    Justin Taraska
    Article
    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1
    Insert
    Clathrin light chain B (CLTB Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Deleted amino acids 207-211
    Promoter
    CMV
    Available since
    Feb. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    193577-plasmid-map-sequence-id-380176

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  5. CLC-EGFP206(flex7)

    Plasmid
    #193578
    Purpose
    Expresses human Clathrin Light Chain B tagged with mEGFP at amino acid position 206 with a flexible linker of 7 amino acids in mammalian cells.
    Depositor
    Justin Taraska
    Article
    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1
    Insert
    Clathrin light chain B (CLTB Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Deleted amino acids 207-211
    Promoter
    CMV
    Available since
    Feb. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    193578-plasmid-map-sequence-id-383666

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  8. pET28a-ybbr-XMod-Doc (BMB-KO, S199AzF)-HIS

    Plasmid
    #153445
    Purpose
    E. coli expression (amber suppression) of Rc. XDocB binding mode B knock-out mutant with serine at position 199 replaced with amber codon.
    Depositor
    Michael Nash
    Article
    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:
    Insert
    Rc.XDocB
    Use
    Tags
    6xHis and ybbr tag
    Expression
    Bacterial
    Mutation
    Serine 199 was mutated to amber codon (TAG). Argi…
    Promoter
    T7 promoter
    Available since
    July 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    153445-plasmid-map-sequence-id-306484

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  9. pET28a-ybbr-XMod-Doc (BMA-KO, S199AzF)-HIS

    Plasmid
    #153444
    Purpose
    E. coli expression (amber suppression) of Rc. XDocB binding mode A knock-out mutant with serine at position 199 replaced with amber codon.
    Depositor
    Michael Nash
    Article
    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:
    Insert
    Rc.XDocB
    Use
    Tags
    6xHis and ybbr tag
    Expression
    Bacterial
    Mutation
    Serine 199 was mutated to amber codon (TAG). Argi…
    Promoter
    T7 promoter
    Available since
    July 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    153444-plasmid-map-sequence-id-306456

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  17. pcDNA5 FRT/TO SF-HERC2 (ShB-R)

    Plasmid
    #55613
    Purpose
    Dox-inducible expression of full length, ShB-resistant, 3xFLAG/STREP tagged wild-type HERC2 in Flp-In TREx cells
    Depositor
    David Chan
    Article
    Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.
    Insert
    3xFLAG tagged HERC2 (WT) (HERC2 Human)
    Use
    Tags
    3xFLAG and STREP
    Expression
    Mammalian
    Mutation
    Silent mutations have been incorporated into the …
    Promoter
    Available since
    July 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    55613-plasmid-map-sequence-id-86499

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  19. his3MX6-ins3

    Plasmid
    #195040
    Purpose
    pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference
    Depositor
    Gloria Brar
    Article
    Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75
    Insert
    His5+
    Use
    Yeast genomic targeting
    Tags
    S. cerevisiae DEG1 terminator, A. gossypii TEF pr…
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    195040-plasmid-map-sequence-id-384243

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Showing: 2501 - 2520 of 42612 results