Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 18,759 results for: nls

Clear keywords
Showing: 2501 - 2520 of 18759 results

  3. DsRed-CPI-17-T38E-NLS

    Plasmid
    #100790
    Purpose
    Mammalian expression of Fluorescent CPI-17 phospho mimetic nuclear
    Depositor
    Joseph Gordon
    Insert
    CPI-17-T38E-NLS (PPP1R14A Human)
    Tags
    DsRed
    Expression
    Mammalian
    Mutation
    T38E
    Promoter
    CMV
    Available since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    100790-plasmid-map-sequence-id-193221

    Enlarge

  4. HA-CPI-17-T38A-NLS

    Plasmid
    #100780
    Purpose
    Mammalian expression of CPI-17 neutral mutant nuclear
    Depositor
    Joseph Gordon
    Insert
    CPI-17-T38A-NLS (PPP1R14A Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    T38A
    Promoter
    CMV
    Available since
    Sept. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    100780-plasmid-map-sequence-id-193203

    Enlarge

  5. HA-CPI-17-T38E-NLS

    Plasmid
    #100778
    Purpose
    Mammalian expression of CPI-17 phospho-mimetic nuclear
    Depositor
    Joseph Gordon
    Insert
    CPI-17-T38E-NLS (PPP1R14A Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    T38E
    Promoter
    CMV
    Available since
    Sept. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    100778-plasmid-map-sequence-id-193198

    Enlarge

  8. lentiCRISPR_DKO

    Plasmid
    #183192
    Purpose
    Plasmid with Cas9, two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen
    Depositor
    Lang Li
    Publication
    Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    hU6, mU6
    Available since
    Aug. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    183192-plasmid-map-sequence-id-360098

    Enlarge

  14. pSLQ2818 pPB: CAG-PYL1-KRAB-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9

    Plasmid
    #84241
    Purpose
    Expresses ABA-inducible KRAB-Sp dCas9
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    ABI-tagBFP-Sp dCas9
    PYL1-KRAB
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    2xNLS (SV40), ABI, HA tag, IRES-Puro, PYL1, and t…
    Expression
    Mammalian
    Promoter
    CAG and PGK
    Available since
    Nov. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    84241-plasmid-map-sequence-id-160882

    Enlarge

  15. FNpCDNA3

    Plasmid
    #45346
    Depositor
    Robert Oshima
    Type
    Empty backbone
    Tags
    FLAG and SV40 NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 14, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    45346-plasmid-map-sequence-id-63229

    Enlarge

  16. hGFAP-Cre

    Plasmid
    #40591
    Purpose
    Mouse astrocyte expression of Cre
    Depositor
    Albee Messing
    Publication
    Zhuo et al Genesis. 2001 Oct;31(2):85-94.
    Insert
    Cre recombinase (GFAP Human, Phage, Mouse)
    Use
    Cre/Lox and Mouse Targeting
    Tags
    MP-1 fragment (mouse protamine 1 intron and polya…
    Expression
    Mammalian
    Mutation
    Construct contains a nuclear-targeted Cre recombi…
    Promoter
    hGFAP promoter
    Available since
    Sept. 20, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    40591-plasmid-map-sequence-id-302078

    Enlarge

  17. pET-28b-T7-hAsCas12a-enRR(E174R/S542R/K607R)-NLS(nucleoplasmin)-6xHis (AAS1902)

    Plasmid
    #114077
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a-enRR(E174R/S542R/K607R) with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a-enRR (E174R/S542R/K607R) with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    E174R/S542R/K607R
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    114077-plasmid-map-sequence-id-220525

    Enlarge

  18. pH-STEME-NG-esgRNA

    Plasmid
    #138136
    Purpose
    Targeted simultaneous C-to-T and A-to-G in rice
    Depositor
    Caixia Gao
    Insert
    APOBEC3A-wtTadA-TadA7.10-nCas9-NG-UGI-NLS
    Expression
    Plant
    Available since
    Sept. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    138136-plasmid-map-sequence-id-311723

    Enlarge

  20. pCMV-T7-ABEmax(7.10)-SpCas9-NG-P2A-EGFP (RTW4564)

    Plasmid
    #140005
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ABEmax(7.10) SpCas9-NG with P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    140005-plasmid-map-sequence-id-275488

    Enlarge

Showing: 2501 - 2520 of 18759 results