We narrowed to 6,447 results for: nls
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Plasmid#227147PurposeVector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with two copies of UGI and hA3A* variant and HMG-D domain was inserted between hA3A* and eIscBn driven by CMV promoterDepositorInsertbpNLS-hA3A*-linker-HMG-D-linker-nOgeuIscB-v3-nucleoplasmin NLS-2XUGI-bpNLS
MutationD96R/E84R/V159R/H339APromoterCMVAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLH_Scr16
Plasmid#100538PurposeCEN/ARS plasmid with PhyBNT-NLS-CreN and PIF3-NLS-CreC expression cassettes for red/far-red light-regulated Cre recombinase activity in Saccharomyces cerevisiae.DepositorUseCre/Lox and Synthetic BiologyTagsCdc1 NLS, CreC, CreN, and SV40 NLSExpressionYeastMutationAA 1-621 of PhyB/ Mutation nucleotide 576 C-->APromoterFBA1 and TDH3Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
A3Ai-Cas9n-UGI-NLS
Plasmid#109425PurposeExpresses human APOBEC3A containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.DepositorInsertApolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A (APOBEC3A Human)
UseCRISPRExpressionMammalianMutationInsertion of an L1 intron into the coding sequenc…PromoterCMVAvailable SinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-endAsCas12a-EGFP-NLS-3xFlag
Plasmid#236363PurposeOverexpression of endAsCas12a in human cellsDepositorInsertendAsCas12a
UseCRISPR and LentiviralMutationE174R, S542R, K548RAvailable SinceJuly 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-HyperdAsCas12a-EGFP-NLS-3xFlag
Plasmid#236362PurposeOverexpression of HyperdAsCas12a in human cellsDepositorInsertHyperdAsCas12a
UseCRISPR and LentiviralMutationE174R, D256R, E321R, E371RAvailable SinceJuly 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dAsCas12a-EGFP-NLS-3xFlag
Plasmid#236361PurposeOverexpression of dAsCas12a in human cellsDepositorInsertdAsCas12a
UseCRISPR and LentiviralMutationWTAvailable SinceJuly 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
5aa SunTag VP64 AP3
Plasmid#115484PurposeCRISPR-Cas9 SunTag system to target VP64 to the AP3 locus with two guide RNAsDepositorInsertg2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pubc-FRB-BFP-IRES-FKBP-HaloTag_tdMCP-NLS-HA (RIDR negative control)
Plasmid#224133PurposeRIDR negative control.DepositorInsertpubc-FRB-tagBFP-IRES-FKBP-HaloTag_tdMCP-NLS-HA
TagsFKBP-HaloTag-tdMCP-NLS-HA and FRB-tagBFPExpressionMammalianPromoterubcAvailable SinceMay 13, 2026AvailabilityAcademic Institutions and Nonprofits only -
tet3G-cmv-FRB-SMG7C-IRES-FKBP-HaloTag_tdMCP-NLS-HA (tet3G-RIDR)
Plasmid#232461PurposeDox-inducible, high expression RIDRDepositorInserttet3G-cmv-FRB-SMG7C-IRES-FKBP-HaloTag-tdMCP-NLS-HA
TagsFKBP-HaloTag-tdMCP-NLS-HA and FRB-SMG7CExpressionMammalianPromotercmvAvailable SinceMay 13, 2026AvailabilityAcademic Institutions and Nonprofits only -
pL6puro-pSV40(3)-pmRevErbA-mScaI3-NLS-Pest
Plasmid#240117PurposeA lentiviral (red) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by SV40 promoter fragment.DepositorInsertmurine Nr1d1 promoter+mScarlet-I3-NLS-Pest-Reporter (Nr1d1 Mouse)
UseLentiviralMutationadded SV40-EnhancerAvailable SinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Synapsin-SspB-EGFP-VP16-P2A-FLAG-TetR-NLS-CapN-SsrA-CapC
Plasmid#213535PurposeExpresses the split transcription factors in cultured neuron and mouse brainDepositorInsertSspB-EGFP-VP16-P2A-FLAG-TetR-NLS-CapN-SsrA-CapC-HA-HA
UseAAVTagsEGFP and HAExpressionMammalianPromoterSynapsinAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSynapsin_NLS_GFP_P2A_SNAP_NR1
Plasmid#170458PurposeExpresses SNAP tagged NR1 in neuronal cellsDepositorAvailable SinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCVL.SFFV.Kozak.HA.NLS.Y2AniK227M.IRES.BFP
Plasmid#45579PurposeExpresses K227M nickase variant of I-Ani I nuclease in mammalian cells with BFP trackerDepositorInsertcoY2 Ani K227M
UseLentiviralTagsHA, IRES BFP, and NLSExpressionMammalianMutationco indicates codon optimization for mammalian exp…PromoterSFFVAvailable SinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
LP102: pMAGIC (R4-R3) NLS-Sp Cas9-NLS
Plasmid#132927PurposepMAGIC R4-R3 entry plasmid, contains 2xNLS SpCas9 (nuclease active) for 3 or 4-component MultiSite Gateway Pro assembly.DepositorInserthumanized SpCas9
UseCRISPR, Gateway Cloning, and Synthetic BiologyAvailable SinceOct. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGTag-NLS-eGFP-SV40
Plasmid#117811PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInserteGFP
UseSynthetic BiologyTagsNLSAvailable SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGTag-NLS-TagRFP-SV40
Plasmid#117805PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertTagRFP
UseSynthetic BiologyTagsNLSAvailable SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBCCP-GFP-NLS
Plasmid#173923PurposeFluorescent labeling of the nuclear envelopeDepositorInsertBiotin carboxyl carrier protein, AcGFP1
Tags3xNLSExpressionMammalianPromoterCMVAvailable SinceSept. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-21a_2xNLS_LbCpf1_protein_expression
Plasmid#114366PurposeProtein expression plasmid for 2xNLS LbCpf1 in pET-21a backboneDepositorInsert2xNLS_LbCpf1
UseCRISPRTagsNLSExpressionBacterialPromoterT7Available SinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX601-AAV-CMV-NLS-SaCas9-NLS-3xHA-bGHpA;U6-Cre-1
Plasmid#237890PurposeCre-KO AAV vector#1 containing SaCas9 and its sgRNA targeting CreDepositorInsertsgRNA-Cre
UseAAV and CRISPRExpressionMammalianPromoterU6Available SinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-fDIO-OFF-ArgiNLS-AausFP1
Plasmid#220605PurposeFlp-independent expression of a single-cell discriminating version of AausFP1 fluorescent protein. Flp activity can turn off expression.DepositorInsertAausFP1
UseAAVTagsArgiNLSExpressionMammalianPromoterCAGAvailable SinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only