We narrowed to 6,573 results for: tac

1. pBRα-σ70 D581G

   Plasmid

   #53732

   Purpose

   Plpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli σ70 region 4. Used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.

   Depositor

   Ann Hochschild

   Article

   Dove et al Nature. 1997 Apr 10;386(6625):627-30.

   Insert

   RNAP alpha NTD (aa 1-248) fused to E. coli sigma 70 (aa 528-613)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   The sigma 70 moiety carries the D581G substitution

   Promoter

   lpp/lacUV5 (tandem promoter)

   Available since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

2. GB-Dcp1b

   Plasmid

   #153979

   Purpose

   Dimerization dependent fluorescent protein GB core domain fused to mRNA decapping enzyme 1b

   Depositor

   Gia Voeltz

   Article

   Lee et al Science. 2020 Jan 31;367(6477). pii: 367

   Insert

   mRNA decapping enzyme 1b (DCP1B Human)
   

   Use

   Tags

   pAcGFP-C1

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCD5-D/bovine/Nebraska/9-5/2012-HEFwtED-GCN4-sfGFP-ST

   Plasmid

   #175019

   Purpose

   Expresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFP

   Depositor

   Robert de Vries

   Article

   Nemanichvili et al Viruses. 2021 Mar 31;13(4). pii: v130405

   Insert

   D/bovine/Nebraska/9-5/2012
   

   Use

   Tags

   GCN4-TEV-sfGFP-TwinStrep

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(-)-EGFP-FP4-mito

   Plasmid

   #229692

   Purpose

   Transient expression of EGFP FP4-mito in mammalian cells

   Depositor

   Alpha Yap

   Article

   Scott et al Mol Biol Cell. 2006 Mar;17(3):1085-95. d

   Insert

   EGFP; Mitochondira FP4 motifs from Listeria ActA
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1(-)-EGFP-AP4-mito

   Plasmid

   #229693

   Purpose

   Transient expression of EGFP AP4-mito in mammalian cells

   Depositor

   Alpha Yap

   Article

   Scott et al Mol Biol Cell. 2006 Mar;17(3):1085-95. d

   Insert

   EGFP; Control construct with tandem AP4 motifs (mitochondria)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. bdSUMO-GFP-HiBit

   Plasmid

   #170989

   Purpose

   For bacterial expression of bdSUMO tagged muGFP with NanoLuc complementation peptide variant #86

   Depositor

   Angus Johnston

   Article

   Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

   Insert

   14x-His-bdSUMO-muGFP-HiBiT
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV.hSyn.Cre

   Plasmid

   #170367

   Purpose

   AAV vector expressing Cre under human synapsin promotor

   Depositor

   Johan Jakobsson

   Article

   Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

   Insert

   Cre
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   human Synapsin

   Available since

   Jan. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. E[beta]P

   Plasmid

   #24313

   Depositor

   Roel Nusse

   Article

   Fuerer et al PLoS One. 2010 . 5(2):e9370.

   Insert

   EF1alpha-betacatenin(deltaGSK) // SV40-PuroR (Ctnnb1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Sites phosphorylated by GSK-3beta have been mutat…

   Promoter

   Available since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSmac-GFP

   Plasmid

   #40881

   Depositor

   Douglas Green

   Article

   Tait et al Dev Cell. 2010 May 18;18(5):802-13.

   Insert

   Smac-GFP (DIABLO Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

10. PLKO.1-G3BP1-3'UTR

    Plasmid

    #136038

    Purpose

    G3BP1 shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (GCCTGTAAGAAATACAGGATT)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    G3BP1 (G3BP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TagBFP2-C1-SACM1LdeltaTMD-C389S-Fis1tail

    Plasmid

    #220079

    Purpose

    SAC1 with a C389S mutation in it with the TMD deleted and replaced with the Fis1tail to replace the TMD and target the mitochondria instead of the ER with a Blue Fluorophore

    Depositor

    Gerry Hammond

    Article

    Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

    Insert

    SACM1l-C389S-Fis1tail (SACM1L Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C389SdeltaTMD

    Promoter

    Available since

    May 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLEX303 GFP-PIK3C2B

    Plasmid

    #162001

    Purpose

    Lentiviral expression vector containing PIK3C2B tagged with GFP

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

    Insert

    PIK3C2B (PIK3C2B Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mutation

    siRNA resistance to siRNA#2 with 4 silent mutatio…

    Promoter

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLentiCRISPR_zeo_sgNT135

    Plasmid

    #183181

    Purpose

    pLentiCRISPR that is selectable with zeocin with a non-targeting sgRNA

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    NT135
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. HA-merlin

    Plasmid

    #32836

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

    Insert

    Merlin (NF2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

15. GFP-Dcp1b

    Plasmid

    #153976

    Purpose

    Fluorescent protein fused to mRNA decapping enzyme 1b

    Depositor

    Gia Voeltz

    Article

    Lee et al Science. 2020 Jan 31;367(6477). pii: 367

    Insert

    mRNA recapping enzybe 1b (DCP1B Human)
    

    Use

    Tags

    pAcGFP-C1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRR-ERbeta-5Z

    Plasmid

    #23062

    Depositor

    Charles Miller

    Article

    Miller et al Plasmid. 2009 Dec 3. ():.

    Insert

    ERbeta (ESR2 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pLV-CMV-tagRFP-Mad2

    Plasmid

    #114021

    Purpose

    Lentiviral delivery of tagRFP-MAD2 expressed from the CMV promoter

    Depositor

    Geert Kops

    Article

    Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

    Insert

    tagRFP-Mad2 (MAD2L1) (MAD2L1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-SRGAP1

    Plasmid

    #187269

    Purpose

    Expression of SRGAP1-EGFP

    Depositor

    Alpha Yap

    Article

    Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

    Insert

    SRGAP1 (SRGAP1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SNAP-Dcp2

    Plasmid

    #153975

    Purpose

    SNAP-tag polypeptide fused to mRNA decapping enzyme 2

    Depositor

    Gia Voeltz

    Article

    Lee et al Science. 2020 Jan 31;367(6477). pii: 367

    Insert

    mRNA Decapping enzyme 2 (DCP2 Human)
    

    Use

    Tags

    SNAP-C1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTC392

    Plasmid

    #91223

    Purpose

    protoplast vector expressing gRNA23 targeting tomato ANT1 (control without TREX2 expression)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    gRNA targeting tomato ANT1
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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