We narrowed to 5,443 results for: SPI

1. pCMV_NFL(K363TAG)-FLAG

   Plasmid

   #182656

   Purpose

   Expresses mouse neurofilament light chain with a TAG codon at the position K363 & C-terminal FLAG tag (DYKDDDDK) in mammalian cells. Can be used for amber codon suppression & click labeling of NFL.

   Depositor

   Ivana Nikić-Spiegel

   Article

   Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

   Insert

   mouse neurofilament light chain with a K363TAG mutation (Nefl Mouse)
   

   Use

   Tags

   FLAG tag (DYKDDDDK)

   Expression

   Mammalian

   Mutation

   K363TAG

   Promoter

   CMV

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCLHCX-mPM20D1-flag

   Plasmid

   #84566

   Purpose

   mouse PM20D1, C-term flag and his tags, in mammalian retroviral expression plasmid. Amp/Hygro selection

   Depositor

   Bruce Spiegelman

   Article

   Long et al Cell. 2016 Jul 14;166(2):424-35. doi: 10

   Insert

   peptidase M20 domain containing 1 (Pm20d1 Mouse)
   

   Use

   Retroviral

   Tags

   6xHis and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTwist-SARS-CoV-2 Δ18 B.1.351v2

   Plasmid

   #169463

   Purpose

   Encodes SARS-CoV-2 B.1.351v2 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

   Insert

   SARS-CoV-2 B.1.351v2 Spike truncated to remove 18 amino acids (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTwist-SARS-CoV-2 Δ18 B.1.1.7

   Plasmid

   #169459

   Purpose

   Encodes SARS-CoV-2 B.1.1.7 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

   Insert

   SARS-CoV-2 B.1.1.7 Spike truncated to remove 18 amino acids (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTwist-SARS-CoV-2

   Plasmid

   #164435

   Purpose

   Encodes SARS-CoV-2 Spike Protein for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

   Insert

   SARS-CoV-2 Spike (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET24a-VHH-2xTS

   Plasmid

   #109419

   Purpose

   Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to two tyrosine sulfation (TS) motifs. VHH-2xTS also contains a T7, HA, BAP and His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

   Insert

   anti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
   

   Use

   Tags

   BAP, HA, His6, T7, and Tyrosine sulfation (TS) se…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHDM-SARS-CoV-2

   Plasmid

   #164433

   Purpose

   Encodes SARS-CoV-2 Spike Protein for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

   Insert

   SARS-CoV-2 Spike (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV.E2.ChETA

   Plasmid

   #220310

   Purpose

   Directs ChETA optogenetic actuator to fast spiking interneurons in cortex. Allows for single APs to be evoked.

   Depositor

   Matt Rowan

   Article

   Kumar et al Nat Commun. 2024 Apr 1;15(1):2823. doi:

   Insert

   ChETA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   S5E2

   Available since

   June 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTwist-SARS-CoV-2 Δ18 B.1.351v3

   Plasmid

   #169464

   Purpose

   Encodes SARS-CoV-2 B.1.351v3 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

   Insert

   SARS-CoV-2 B.1.351v3 Spike truncated to remove 18 amino acids (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA Flag PGC1 T177A S538A

    Plasmid

    #18093

    Depositor

    Bruce Spiegelman

    Article

    Jager et al Proc Natl Acad Sci U S A. 2007 Jul 17. 1

    Insert

    PGC-1a (Ppargc1a Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    T177A S538A

    Promoter

    Available since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only