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xfz3N/pCS2+

Plasmid

#16328

Depositor

Peter Klein

Article

Deardorff et al Development. 2001 Oct . 128(19):3655-63.

Insert

frizzled-3 (fzd3.L Frog)

Use

Vertebrate expression

Mutation

Extracellular ligand binding domain of Xenopus fr…

Available Since

Dec. 21, 2007

Availability

Academic Institutions and Nonprofits only
-67 hTF

Plasmid

#15452

Depositor

Nigel Mackman

Article

Mackman et al FASEB J. 1995 Jul . 9(10):883-9.

Insert

Tissue factor promoter (F3 Human)

Use

Luciferase

Expression

Mammalian

Available Since

July 27, 2007

Availability

Academic Institutions and Nonprofits only

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pLG371

Plasmid

#128410

Purpose

Cuminic acid-inducible system

Depositor

Christopher Voigt

Article

Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

Insert

gfpmut2 (NEWENTRY Synthetic)

Expression

Bacterial

Promoter

Pspank(C)

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLG231

Plasmid

#124369

Purpose

IPTG inducible system (gfpmut2 with Ppsank and riboJ) for expression in B. subtilis

Depositor

Christopher Voigt

Article

Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

Insert

gfpmut2

Expression

Bacterial

Promoter

Pspank

Available Since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-Notch5#29

Plasmid

#22415

Depositor

Nathan Lawson

Article

Lawson et al Development. 2001 Oct . 128(19):3675-83.

Insert

Notch5

Use

Riboprobe

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
pTU-B-005

Plasmid

#124413

Purpose

Slot B, contains sfGFP-MarAn20 (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot B with sfGFP-MarAn20 reporter + terminator + spacer

Expression

Bacterial

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTU-C-005

Plasmid

#124414

Purpose

Slot C, contains RepA70-mKate2 (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot C with RepA70-mKate2 reporter + terminator

Expression

Bacterial

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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K218Tc-3

Plasmid

#1300

Depositor

Susan Lindquist

Article

Schirmer et al Proc Natl Acad Sci U S A 2001 Jan 30;98(

Insert

mutant HSP104 (HSP104 Budding Yeast)

Expression

Yeast

Mutation

K218T: A315T

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pLIC.B4.SPy0289

Plasmid

#11534

Depositor

Sung-Hou Kim

Article

Liu et al Proteins. 2005 Jun 1. 59(4):875-81.

Insert

IscU

Tags

HisTag-MBP, TEV cleavable

Expression

Bacterial

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only
p39.pA.NGFR.mCMV.hPGK.SrtA(Pasqual).WPRE[sequenced]

Plasmid

#226758

Purpose

secreted SrtA in p36

Depositor

Ferdinando Pucci

Article

Hamilton et al Adv Biosyst. 2020 Dec;4(12):e2000007. do

Insert

sortase A (srtA )

Expression

Mammalian

Available Since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only

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p33.pA.NGFR-mSrtA(Pasqual)-flag.mCMV.hPGK.GFP.wpre[sequenced]

Plasmid

#226730

Purpose

sortase A(Pasqual) in p30

Depositor

Ferdinando Pucci

Article

Hamilton et al Adv Biosyst. 2020 Dec;4(12):e2000007. do

Insert

sortase A (srtA Synthetic)

Expression

Mammalian

Available Since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only

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p35.pA.NGFR.mCMV.hPGK.mSrtA(Pasqual)TM.WPRE[sequenced]

Plasmid

#226760

Purpose

sortase A (Pasqual)- TM in p36

Depositor

Ferdinando Pucci

Article

Hamilton et al Adv Biosyst. 2020 Dec;4(12):e2000007. do

Insert

sortase A (srtA )

Expression

Mammalian

Available Since

Oct. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-ABCC1_K684I_STOP

Plasmid

#221430

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Insert

ABCC1 (ABCC1 Human)

Expression

Mammalian

Mutation

K684I

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-ABCC1_S769N_STOP

Plasmid

#221431

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Insert

ABCC1 (ABCC1 Human)

Expression

Mammalian

Mutation

S769N

Available Since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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NF2A-c001

Plasmid

#175472

Purpose

Protein expression in bacterial cells. FERM domain, M1-R338.

Depositor

Opher Gileadi

Insert

NF2 (NF2 Human)

Tags

His6-TEV

Expression

Bacterial

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pTU-A-010

Plasmid

#124418

Purpose

Slot A, contains mKO2-MarA (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot A with mKO2-MarA reporter + terminator + spacer

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTU-B-010

Plasmid

#124419

Purpose

Slot B, contains sfGFP-MarAn20 (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot B with sfGFP-MarAn20 reporter + terminator + spacer

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTU-C-010

Plasmid

#124420

Purpose

Slot C, contains RepA70-mKate2 (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot C with RepA70-mKate2 reporter + terminator

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pBS xenopus Endodermin (pBS-eddL6)

Plasmid

#24968

Depositor

Edward De Robertis

Article

Sasai et al EMBO J. 1996 Sep 2. 15(17):4547-55.

Insert

Endodermin (a2m Frog)

Expression

Bacterial

Available Since

June 29, 2010

Availability

Academic Institutions and Nonprofits only
pLG173

Plasmid

#124368

Purpose

Control plasmid with only spectinomycin

Depositor

Christopher Voigt

Article

Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

Insert

empty backbone (control) (NEWENTRY Synthetic)

Expression

Bacterial

Promoter

No promoter

Availability

Academic Institutions and Nonprofits only

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