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pOPINK-CAPN15 NZF2 (34-72)

Plasmid

#235645

Purpose

Bacterial expression of human CAPN15 NZF2 (34-72)

Depositor

David Komander

Article

Michel et al Cell Rep. 2024 Aug 27;43(8):114545. doi:

Insert

CAPN15 NZF2 (CAPN15 Human)

Tags

6xHis-GST-3c

Expression

Bacterial

Mutation

aa 34-72 only

Promoter

T7

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pOPINK-CAPN15 NZF1 (1-42)

Plasmid

#235644

Purpose

Bacterial expression of human CAPN15 NZF1 (1-42)

Depositor

David Komander

Article

Michel et al Cell Rep. 2024 Aug 27;43(8):114545. doi:

Insert

CAPN15 NZF1 (CAPN15 Human)

Tags

6xHis-GST-3c

Expression

Bacterial

Mutation

aa 1-42 only

Promoter

T7

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-hM3D(Gq)-mCherry-shScg2-CWB

Plasmid

#223659

Purpose

Expresses hM3D(Gq) and a shRNA targetting Scg2 in a Cre-dependent manner

Depositor

Oscar Marin

Article

Selten et al Nature. 2025 Apr 30. doi: 10.1038/s41586

Insert

hM3D(Gq)-mCherry and shScg2

Use

AAV and RNAi

Tags

mCherry

Expression

Mammalian

Promoter

hSyn

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-FLAG-APEX2-NES-K.l.LEU2

Plasmid

#229229

Purpose

Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

Depositor

Fulvio Reggiori

Article

Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-FLAG-APEX2-NES-TRP1

Plasmid

#229228

Purpose

Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

Depositor

Fulvio Reggiori

Article

Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro human RLIM N581K

Plasmid

#232242

Purpose

Mammalian expression of Human RLIM N581K

Depositor

Francisco Bustos

Article

Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

Insert

Human RLIM N581K (RLIM Human)

Expression

Mammalian

Mutation

N581K

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX6P1 human RLIM N581K

Plasmid

#232241

Purpose

Protein expression of GST-human RLIM N581K

Depositor

Francisco Bustos

Article

Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

Insert

GST-human RLIM N581K (RLIM Human)

Tags

GST

Expression

Bacterial

Mutation

N581K

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pGG-PIP02

Plasmid

#196740

Purpose

Contains PEP2 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

PLANT ELICITOR PEPTIDE 2 (PROPEP2 Mustard Weed)

Use

Greengate (gg) entry vector

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP03

Plasmid

#196741

Purpose

Contains PEP3 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

PLANT ELICITOR PEPTIDE 3 (PROPEP3 Mustard Weed)

Use

Greengate (gg) entry vector

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-D6Y_AroPERFECT

Plasmid

#215634

Purpose

For overexpression of Gal4-DBD D6Y AroPERFECT

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

D6Y AroPERFECT

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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BoxB-MS2 adRNA (RAB7A-GAC site)

Plasmid

#170149

Purpose

AAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcript

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

BoxB-RAB7A(GAC)-MS2

Use

AAV

Expression

Mammalian

Promoter

Human U6

Available Since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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MS2-adRNA (RAB7A-GAC site)

Plasmid

#170140

Purpose

AAV vector carrying a MS2 adRNA targeting the RAB7A transcript

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MS2-RAB7A(GAC)-MS2

Use

AAV

Expression

Mammalian

Promoter

Human U6

Available Since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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CMV DJ-1 C106A

Plasmid

#220914

Purpose

Expresses DJ-1 with the C106A mutation

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Insert

DJ-1 (PARK7 Human)

Expression

Mammalian

Mutation

C106A

Promoter

CMV

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pKL2-138

Plasmid

#219413

Purpose

Estradiol-inducible expression of 42-10 Zinc finger activator

Depositor

Ahmad Khalil

Article

Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

Insert

{pTEF1-ZEV}{pGal1(Zif268op)x6-3xFLAG-NLS-VP16-mRuby2-(42-10-4x)-PDZLigWT-tSSA1}

Expression

Yeast

Mutation

Four Arg to Ala mutations in zinc finger backbone

Available Since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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pGal4-DBD-OCT4_WT_N

Plasmid

#215622

Purpose

For overexpression of Gal4-DBD OCT4 WT N

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

OCT4 N

Expression

Mammalian

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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rSirt3 shRNA (pLKO.1 vector)

Plasmid

#216807

Purpose

shRNA against rat Sirt3

Depositor

Ghazaleh Ashrafi

Article

Tiwari et al J Cell Biol. 2024 Jan 1;223(1):e20230504

Insert

Sirt3 (Sirt3 Rat)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-S6Y_AroPERFECT

Plasmid

#215631

Purpose

For overexpression of Gal4-DBD S6Y AroPERFECT

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

S6Y AroPERFECT

Expression

Mammalian

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGal4-DBD-OCT4_AroPERFECT_C

Plasmid

#215625

Purpose

For overexpression of Gal4-DBD OCT4 AroPERFECT C

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

OCT4 AroPERFECT C

Expression

Mammalian

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-S6Y_AroPATCHY3

Plasmid

#215632

Purpose

For overexpression of Gal4-DBD S6Y AroPATCHY 3

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

S6Y AroPATCHY 3

Expression

Mammalian

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pGal4-DBD-OCT4_WT_C

Plasmid

#215623

Purpose

For overexpression of Gal4-DBD OCT4 WT C

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

OCT4 C

Expression

Mammalian

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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