We narrowed to 17,075 results for: Por

1. pB80-SSPB(micro)-mVenus-ppKin14VIb(861-1321)

   Plasmid

   #174641

   Purpose

   Optogenetic coupling to dimeric moss kinesin-14 for retrograde transport via SSPB(micro)

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   SSPB(micro)-mVenus-ppKin14VIb(861-1321)
   

   Tags

   SSPB(micro)-Venus

   Expression

   Mammalian

   Mutation

   SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…

   Promoter

   Chicken beta-actin

   Available Since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. UAS-u(XXL)Cas9

   Plasmid

   #127387

   Purpose

   Expresses Cas9 at very low level

   Depositor

   Michael Boutros

   Article

   Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

   Insert

   uORF(XXL)-Cas9
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Antares-SEPLuc C1A4E

   Plasmid

   #141088

   Purpose

   precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRES and weaker precursor of Nanoluc

   Depositor

   Jeak Ling Ding

   Article

   Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

   Insert

   Antares-T2A-puromycin-IRESv24-SEP-C1A4E
   

   Expression

   Mammalian

   Mutation

   Please see depositor comments

   Promoter

   CMV

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-4T-3-cytXlATL2

   Plasmid

   #124063

   Purpose

   Fragment of Xenopus laevis Atlastin (cytATL). This fragment contains the GTPase domain and the three-helix bundle, but lacks the transmembrane segments and the cytosolic tail.

   Depositor

   Tom Rapoport

   Article

   Wang et al J Cell Biol. 2013 Dec 9;203(5):801-14. d

   Insert

   cytXIATL2
   

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pProEx-Htb-cytATL2(R323Q)-6Gly-sfGFP

   Plasmid

   #124066

   Purpose

   E. coli. exp. and purif. of X. laevis cytATL-R232Q fragment fused to GFP in c-terminus.

   Depositor

   Tom Rapoport

   Article

   Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

   Insert

   cytATL-R232Q-sfGFP
   

   Tags

   sfGFP

   Expression

   Bacterial

   Mutation

   R232Q

   Promoter

   trc

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. zebrafish SLC2A2 (zfSLC2A2)

   Plasmid

   #65103

   Purpose

   Full length SLC2A2 cDNA (Danio rerio)

   Depositor

   Josep Planas

   Article

   Castillo et al Am J Physiol Regul Integr Comp Physiol.

   Insert

   SLC2A2 (slc2a2 Zebrafish)
   

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Jan. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

7. p3 FLAG_CMV14/OCTN2 (delta554_557/PDZ)

   Plasmid

   #112803

   Purpose

   Expression of OCTN2 mutant

   Depositor

   Katarzyna Nalęcz

   Article

   Jurkiewicz et al Biochim Biophys Acta. 2017 May;1864(5):7

   Insert

   OCTN2/SLC22A5 (Slc22a5 Rat)
   

   Tags

   3 x Flag

   Expression

   Mammalian

   Mutation

   amino acids 554_557 deleted/PDZ

   Available Since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. p3 FLAG_CMV14/OCTN2 (delta14_22)

   Plasmid

   #112800

   Purpose

   Expression of OCTN2 mutant

   Depositor

   Katarzyna Nalęcz

   Article

   Czeredys et al PLoS One. 2013 Dec 13;8(12):e82105. doi:

   Insert

   OCTN2/SLC22A5 (Slc22a5 Rat)
   

   Tags

   3 x Flag

   Expression

   Mammalian

   Mutation

   amino acids 14_22 deleted

   Available Since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL2-MCT2Prom2.5

   Plasmid

   #87351

   Purpose

   Human monocarboxylate transporter 2 (MCT2) promoter 2.5 kbp

   Depositor

   Saroj Mathupala

   Article

   Caruso et al Neoplasia. 2017 Jan 13;19(2):121-134. do

   Insert

   MCT2 (SLC16A7 Human)
   

   Use

   Luciferase

   Expression

   Bacterial and Mammalian

   Promoter

   2.5 kbp proximal promoter of human MCT2 (nested d…

   Available Since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL2-MCT2Prom1.1

    Plasmid

    #87352

    Purpose

    Human monocarboxylate transporter 2 (MCT2) promoter 1.1 kbp

    Depositor

    Saroj Mathupala

    Article

    Caruso et al Neoplasia. 2017 Jan 13;19(2):121-134. do

    Insert

    MCT2 (SLC16A7 Human)
    

    Use

    Luciferase

    Expression

    Bacterial and Mammalian

    Promoter

    1.1 kbp proximal promoter of human MCT2 (nested d…

    Available Since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL2-MCT2Prom3.2

    Plasmid

    #87349

    Purpose

    Human monocarboxylate transporter 2 (MCT2) promoter 3.2 kbp

    Depositor

    Saroj Mathupala

    Article

    Caruso et al Neoplasia. 2017 Jan 13;19(2):121-134. do

    Insert

    MCT2 (SLC16A7 Human)
    

    Use

    Luciferase

    Expression

    Bacterial and Mammalian

    Promoter

    3.2 kbp proximal promoter of MCT2 (nested deletio…

    Available Since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. p1G108CFa

    Plasmid

    #85089

    Purpose

    Expresses PCNA1 variant (G108C) fused to an FAD domain of P450 BM3 (A74G/C773S/C810S) in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    The G108C variant of PCNA1 fused to an FAD domain of P450 BM3
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    G108C in PCNA1, A74G/C773S/C810S in FAD domain of…

    Promoter

    T7 promoter

    Available Since

    Nov. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEMS1241

    Plasmid

    #29123

    Purpose

    Expression of the eGFP reporter gene was not detected in the brain and eye.

    Depositor

    Elizabeth Simpson

    Article

    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

    Insert

    Ple12
    

    Tags

    EGFP-NLS

    Available Since

    Nov. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. UAS-u(XS)Cas9

    Plasmid

    #127382

    Purpose

    Expresses Cas9 at very high level

    Depositor

    Michael Boutros

    Article

    Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

    Insert

    uORF-Cas9
    

    Use

    CRISPR

    Expression

    Insect

    Availability

    Academic Institutions and Nonprofits only

    

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15. M51 Super 8x FOPFlash (TOPFlash mutant)

    Plasmid

    #12457

    Purpose

    Control for beta-catenin reporter. Contains mutated TCF/LEF binding sites upstream of a luciferase reporter.

    Depositor

    Randall Moon

    Article

    Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

    Insert

    mutant TCF/LEF binding sites (Ctnnb1 )
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    mutant TCF/LEF binding sites

    Available Since

    March 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEvol-pAzFRS.2.t1

    Plasmid

    #73546

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

    Depositor

    Farren Isaacs

    Article

    Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

    Inserts

    pAzFRS.2.t1
    pAzFRS.2.t1
    

    Expression

    Bacterial

    Available Since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKrox24(MapErk)DsRed

    Plasmid

    #200114

    Purpose

    Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    MapErk sequence in minimal promoter
    

    Expression

    Mammalian

    Mutation

    highly modified sequence

    Promoter

    5 copies of designed MapErk sequence, no other pr…

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE2 GFP-Rtn4a

    Plasmid

    #86684

    Purpose

    Lentivirus to stably express fluorescent human protein in mammalian cells

    Depositor

    Tom Rapoport

    Article

    Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

    Insert

    Rtn4a (RTN4 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEvol-pAcFRS.2.t1

    Plasmid

    #73544

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

    Depositor

    Farren Isaacs

    Article

    Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

    Inserts

    pAcFRS.2.t1
    pAcFRS.2.t1
    

    Expression

    Bacterial

    Available Since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSPObooster

    Plasmid

    #216160

    Purpose

    Plasmid expressing the RME1(ins-308A) and MKT1(30G) genes from S. cerevisiae to correct sporulation defect of S288C derived yeast lab strains

    Depositor

    Grant Brown

    Article

    Loll-Krippleber et al Yeast. 2024 Sep 9. doi: 10.1002/yea.3978

    Inserts

    MKT1(30G) (MKT1 Budding Yeast)
    RME1(ins-308A) (RME1 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    MKT1 gene containing an aspartic acid to glycine …

    Promoter

    endogenous promoter

    Available Since

    May 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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