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MMW3001 - human expression plasmid for SpCas9 Cluster 2 + Q926A

Plasmid

#101184

Purpose

Human expression plasmid for SpCas9 Cluster 2 + Q926A variant: CMV-T7-hSpCas9-Cluster2+Q926A(G582A, V583A, E584A, D585A, N588A, Q926A)-NLS(SV40)-3xFLAG

Depositor

Keith Joung, Jennifer Doudna

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

hSpCas9-Cluster2+Q926A(G582A/V583A/E584A/D585A/N588A/Q926A)

Use

Tags

3x FLAG and NLS (SV40)

Expression

Mutation

G582A/V583A/E584A/D585A/N588A/Q926A

Promoter

CMV

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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MMW3709 - human expression plasmid for SpCas9 Cluster 1 + Q926A

Plasmid

#101179

Purpose

Human expression plasmid for SpCas9 Cluster 1 + Q926A variant: CMV-T7-hSpCas9-Cluster1+Q926A(N692A, M694A, Q695A, H698A, Q926A)-NLS(SV40)-3xFLAG

Depositor

Keith Joung, Jennifer Doudna

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

hSpCas9-Cluster1+Q926A(N692A/M694A/Q695A/H698A/Q926A)

Use

Tags

3x FLAG and NLS (SV40)

Expression

Mammalian

Mutation

N692A/M694A/Q695A/H698A/Q926A

Promoter

CMV

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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MMW3914 - human expression plasmid for SpCas9 Cluster 1 H698 + Q926A

Plasmid

#101180

Purpose

Human expression plasmid for SpCas9 Cluster 1 H698 + Q926A variant: CMV-T7-hSpCas9-Cluster1H698+Q926A(N692A, M694A, Q695A, Q926A)-NLS(SV40)-3xFLAG

Depositor

Keith Joung, Jennifer Doudna

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

hSpCas9-Cluster1H698+Q926A(N692A/M694A/Q695A/Q926A)

Use

Tags

3x FLAG and NLS (SV40)

Expression

Mammalian

Mutation

N692A/M694A/Q695A/Q926A

Promoter

CMV

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF4D03

Plasmid

#70969

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Taf12 (Taf12 Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_CUS1F03

Plasmid

#71071

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Su(var)205 (Su(var)205 Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only
pWZLblasti-HA-MEK5 CBM-E

Plasmid

#53169

Purpose

Expresses HA tagged human MEK5 L280AH281AM323AQ332A from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK5 (MAP2K5 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

L280A/H281A/M323A/Q332A

Promoter

Available since

Sept. 19, 2014

Availability

Academic Institutions and Nonprofits only
PB-tetO-NGN2

Plasmid

#197090

Purpose

PiggyBac plasmid with tet-inducible expression of transcription factors for cortical neuron differentiation

Depositor

Brian Wainger

Article

Held et al Cell Rep. 2023 Aug 30;42(9):113046. doi:

Insert

NGN2 (NEUROG2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pX330 p53

Plasmid

#59910

Purpose

pX330 backbone expressing sgRNA targeting p53 to edit mouse p53. Expresses Cas9 from CBh promoter

Depositor

Tyler Jacks

Article

Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

Insert

sgRNA targeting p53 (Trp53 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pX330 Pten

Plasmid

#59909

Purpose

pX330 backbone expressing sgRNA targeting Pten to edit mouse Pten. Expresses Cas9 from CBh promoter

Depositor

Tyler Jacks

Article

Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

Insert

sgRNA targeting Pten (Pten Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

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pINDUCER-21-RUNX1

Plasmid

#97043

Purpose

Expresses RUNX1 in mammalian cells

Depositor

George Daley

Article

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Runt Related Transcription Factor 1 (RUNX1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Tet on

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only