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pGEX-6P-1-HsTNRC6A_1209-1709-F1359A_S

Plasmid

#147525

Purpose

Bacterial Expression of HsTNRC6A_1209-1709-F1359A

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6A_1209-1709-F1359A (TNRC6A Human)

Use

Tags

Expression

Bacterial

Mutation

one non silent mutation K1578E compared to the se…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-HsTNRC6A_1209-1709-M2WA_S

Plasmid

#147526

Purpose

Bacterial Expression of HsTNRC6A_1209-1709-M2WA

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6A_1209-1709-M2WA (TNRC6A Human)

Use

Tags

Expression

Bacterial

Mutation

one non silent mutation K1578E compared to the se…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsEDC4_974-1401_S

Plasmid

#147529

Purpose

Mammalian Expression of HsEDC4_974-1401

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

Insert

HsEDC4_974-1401 (EDC4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-CeAIN2_254-400-HsTNRC6C_1207-1690_R

Plasmid

#147393

Purpose

Insect Expression of CeAIN2_254-400-HsTNRC6C_1207-1690

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400-HsTNRC6C_1207-1690 (TNRC6C Human, Nematode)

Use

Tags

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpG-HsNot7_R

Plasmid

#147366

Purpose

Bacterial Expression of HsNot7

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot7 (CNOT7 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGW182-12xmut_1-539_O

Plasmid

#147157

Purpose

Insect Expression of DmGW182-12xmut_1-539

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

DmGW182-12xmut_1-539 (gw Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsNot7_L

Plasmid

#146889

Purpose

Mammalian Expression of HsNot7

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsNot7 (CNOT7 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmNot1_J

Plasmid

#146710

Purpose

Insect Expression of DmNot1

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

DmNot1 (Not1 Fly)

Use

Tags

Expression

Insect

Mutation

11 silent mutations compared to the sequence give…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRD424

Plasmid

#178202

Purpose

Expression of Rok from the hns promoter

Depositor

Remus Dame

Article

Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

Insert

phns + rok

Use

Tags

Expression

Bacterial

Mutation

Promoter

hns promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pTSK405

Plasmid

#190817

Purpose

For C-terminus 3xGFP tagging of a gene of interest using URA3 selection marker in S. cerevisiae

Depositor

Gunjan Mehta, Tatiana Karpova

Article

Ball et al Nucleic Acids Res. 2016 Dec 1;44(21):e16

Insert

URA3 (URA3 Budding Yeast)

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pRD410

Plasmid

#178200

Purpose

Expression of sRok from the hns promoter

Depositor

Remus Dame

Article

Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

Insert

phns + srok

Use

Tags

Expression

Bacterial

Mutation

Promoter

hns promoter

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pETM-41P-HsXRN1_1174-1649-Strep_AH

Plasmid

#148901

Purpose

Bacterial Expression of HsXRN1_1174-1649

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2019 Sep 26;47(17):92

Insert

HsXRN1_1174-1649 (XRN1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP2-E148QF404AF407AF409A_V

Plasmid

#147812

Purpose

Mammalian Expression of HsDCP2-E148QF404AF407AF409A

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

Insert

HsDCP2-E148QF404AF407AF409A (DCP2 Human)

Use

Tags

Expression

Mammalian

Mutation

two silent mutations compared to the sequence giv…

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP2-5xmut_V

Plasmid

#147806

Purpose

Mammalian Expression of HsDCP2-5xmut

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

Insert

HsDCP2-5xmut (DCP2 Human)

Use

Tags

Expression

Mammalian

Mutation

two silent mutations compared to the sequence gi…

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP2_97-420_V

Plasmid

#147810

Purpose

Mammalian Expression of HsDCP2_97-420

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

Insert

HsDCP2_97-420 (DCP2 Human)

Use

Tags

Expression

Mammalian

Mutation

one silent mutation compared to the sequence give…

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP2-D15AR19A_V

Plasmid

#147811

Purpose

Mammalian Expression of HsDCP2-D15AR19A

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

Insert

HsDCP2-D15AR19A (DCP2 Human)

Use

Tags

Expression

Mammalian

Mutation

Two silent mutations compared to the sequence giv…

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pWPT-mEGFP

Plasmid

#190606

Purpose

Obtained by creating a deletion inside the mCherry coding sequence in pWPT-/GCCACC-mEGFP-IRES-mCherry (Addgene #49235)

Depositor

Alessandro Quattrone

Article

Ambrosini et al Nucleic Acids Res. 2022 Oct 14;50(18):10

Inserts

mEGFP
mCherry

Use

Lentiviral

Tags

Expression

Mutation

a deletion was created in mCherry CDS impairing i…

Promoter

EIF1-short

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19-sg-1

Plasmid

#190607

Purpose

Expresses a gRNA for base editing the EGFP Kozak sequence of pWPT-/mEGFP-1T-IRES-mCherry

Depositor

Alessandro Quattrone

Article

Ambrosini et al Nucleic Acids Res. 2022 Oct 14;50(18):10

Insert

gRNA sequence

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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Immune checkpoint (library 2) Backbone

Plasmid

#192363

Purpose

Plasmid vector for in vivo immune checkpoint screening library (for checkpoint library)

Depositor

Lijia Ma

Article

Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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T cell activation (library 1) Backbone

Plasmid

#192364

Purpose

Plasmid vector for in vitro Jurkat activation screening (for immune library)

Depositor

Lijia Ma

Article

Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge