We narrowed to 159,481 results for: addgene

1. CRISPR_TRAC

   Plasmid

   #164993

   Purpose

   Expression of gRNA targeting TCRalpha constant locus

   Depositor

   Matthew Meyerson

   Article

   Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

   Insert

   gRNA against TRAC
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. 4xHRE-MinTK-CRE

   Plasmid

   #141144

   Purpose

   lentiviral expression vector to generate a hypoxia fate-mapping system

   Depositor

   Daniele Gilkes

   Article

   Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

   Insert

   4xHRE-MinTK-CRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPTD-DRBD

   Plasmid

   #35622

   Depositor

   Steven Dowdy

   Article

   Eguchi et al Nat Biotechnol. 2009 Jun;27(6):567-71. E

   Insert

   PKR DRBD-1
   

   Use

   Tags

   6xHIS, HA, and TAT

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 15, 2012

   Availability

   Academic Institutions and Nonprofits only

4. U6::sgBFP-U6::sgKrasG12D-pX333-red

   Plasmid

   #149448

   Purpose

   pX333 backbone expressing 2 sgRNA targeting BFP and KRAS G12D to edit mouse KRAS G12D allele.

   Depositor

   Jonathan Chernoff

   Article

   Budagyan et al Methods Mol Biol. 2021;2262:323-334. doi

   Insert

   sgRNA BFP and mouse KRAS G12D
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFA6a-link-ymVenus-URA3

   Plasmid

   #168058

   Purpose

   Replaces Addgene plasmid 118459. Yeast tagging vector to create a gene fusion with yeast optimized mVenus with CaUra3 selection; Contains the linker region at the 5’ end of the FP

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymVenus
   

   Use

   Tags

   ymVenus

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKLV2-EF1a-NucGFP

   Plasmid

   #184696

   Purpose

   Lentiviral transfer plasmid for expression of NucGFP

   Depositor

   Brian Van Tine

   Article

   Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

   Insert

   NucGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT3TS-miniTurboID-EGFP

   Plasmid

   #163847

   Purpose

   In vitro transcription of miniTurboID-EGFP. Parton lab clone JRT

   Depositor

   Rob Parton

   Article

   Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

   Insert

   miniTurbo
   

   Use

   In vitro transcription of rna

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   Sept. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. VV246: CoChR(C108S)-GCaMP6f-ER in fck

   Plasmid

   #58521

   Purpose

   Expresses CoChR(C108S) fused to GCaMP6f in mammalian cells

   Depositor

   Adam Cohen

   Article

   Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

   Insert

   CoChR(C108S)-GCaMP6f-ER
   

   Use

   Lentiviral

   Tags

   GCaMP6f

   Expression

   Mammalian

   Mutation

   changed Cysteine 108 to Serine

   Promoter

   a-CamKII

   Available since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pdCas9-omega

   Plasmid

   #114632

   Purpose

   Addgene plasmid 44249 where dCas9 has been replaced with dCas9 fused to the omega subunit of RNAP

   Depositor

   Anushree Chatterjee

   Article

   Otoupal et al Commun Biol. 2018 Sep 3;1:129. doi: 10.1

   Insert

   dCas9-omega
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Tet promoter

   Available since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pICH-chl-roGFP2-ORP1

    Plasmid

    #191699

    Purpose

    Ti plasmid pICH86988 harbouring chl-roGFP2-ORP1 for peroxide detection in the plastids of plant cells.

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

    Insert

    chl-roGFP2-ORP1
    

    Use

    Binary agrobacterium ti plasmid

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S promoter

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. TET-pLKO.1 PURO shGFP

    Plasmid

    #83085

    Purpose

    Lentiviral shRNA vector for inducible knockdown of GFP (control hairpin)

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    shGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

12. Dopey2_pCSdest

    Plasmid

    #53850

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    Dopey2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6 and CMV

    Available since

    Sept. 8, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pSADdeltaG-GFP-ER(T2)CreER(T2)

    Plasmid

    #32649

    Depositor

    Edward Callaway

    Article

    Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

    Inserts

    GFP
    ER(T2)CreER(T2)
    

    Use

    Rabies virus

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

14. LoriT

    Plasmid

    #163691

    Purpose

    Modified L4440 containing now CamR and oriT for conjugation

    Depositor

    Baris Tursun

    Article

    Kazmierczak et al Nucleic Acids Res. 2021 Feb 26;49(4):e22

    Insert

    oriT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBigTB-FlpERT2

    Plasmid

    #149435

    Purpose

    tamoxifen-inducible Flp recombinase

    Depositor

    Primo Schaer

    Article

    Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

    Insert

    Flp-ERT2 fusion protein
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    ERT2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSLIK 3xFLAG-MTUS1 zeo

    Plasmid

    #192323

    Purpose

    Lentiviral expression vector for an inducible 3xFLAG-MTUS1

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    MTUS1 (MTUS1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. TET-pLKO.1 PURO shLacZ

    Plasmid

    #98632

    Purpose

    Lentiviral shRNA vector for inducible knockdown of LacZ (control hairpin)

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    shLacZ
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR1A_no_ccDB_mUty

    Plasmid

    #158570

    Purpose

    V5-tagged sequence of mUty cloned into entry vector for recombination

    Depositor

    Christian Deschepper

    Article

    Deschepper et al Sci Rep. 2020 Sep 10;10(1):14900. doi: 1

    Insert

    Mus musculus ubiquitously transcribed tetratricopeptide repeat containing, Y-linked (Uty), transcript variant 2 (Uty Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH1

    Plasmid

    #164800

    Purpose

    For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA1

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    ERH CRISPRi gRNA1
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1Alpha and U6

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH2

    Plasmid

    #164801

    Purpose

    For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA2

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    ERH CRISPRi gRNA2
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1Alpha and U6

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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