We narrowed to 4,947 results for: mcs

1. pk335-CARGO-11mer-35kb-DSF-1-11

   Plasmid

   #227489

   Purpose

   11-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

   Depositor

   Joanna Wysocka

   Article

   Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

   Insert

   CARGO to 35kb Downstream Fgf5
   

   Use

   CRISPR

   Available Since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pk335-CARGO-6mer-14kb-USF-1-6

   Plasmid

   #227470

   Purpose

   6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

   Depositor

   Joanna Wysocka

   Article

   Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

   Insert

   CARGO to 14kb Upstream Fgf5
   

   Use

   CRISPR

   Available Since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pk335-CARGO-6mer-14kb-USF-7-12

   Plasmid

   #227471

   Purpose

   6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

   Depositor

   Joanna Wysocka

   Article

   Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

   Insert

   CARGO to 14kb Upstream Fgf5
   

   Use

   CRISPR

   Available Since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pk335-CARGO-12mer-14kb-USF-1-12

   Plasmid

   #227472

   Purpose

   12-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

   Depositor

   Joanna Wysocka

   Article

   Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

   Insert

   CARGO to 14kb Upstream Fgf5
   

   Use

   CRISPR

   Available Since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. EW371 (PB) (ZNF35/ZNF35tar-6bp spacers)x4-H2B-BFP insul CMV-TO-ZNF35(5-8)-ZNF35(5-8)-deimlink-NZF

   Plasmid

   #236173

   Purpose

   PiggyBac-integrated plasmid containing hygromycin resistance gene, ZNF35/ZNF35-driven BFP, and ZNF35/ZNF35-NZF transcription factor

   Depositor

   Xiaojing Gao

   Article

   Wolfsberg et al bioRxiv 2025.02.24.639918

   Inserts

   ZNF35(5-8)-ZNF35(5-8)-deImmunLink-NZF
   H2B (H2BC11 Human)
   

   Tags

   mTagBFP2

   Expression

   Mammalian

   Promoter

   CMV and four ZNF35/ZNF35 sites

   Available Since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVEA3-KNC

   Plasmid

   #234801

   Purpose

   Mammalian expression of bovine arrestin-3 (K11A, K12A, L49A, D51A, R52A, L69A, Y239A, D241A, C252A, P253A, D260A, Q262A)

   Depositor

   Vsevolod Gurevich

   Article

   Breitman et al J Biol Chem. 2012 Jun 1;287(23):19653-64

   Insert

   Arrestin-3 (ARR3 Bovine)
   

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   K11A, K12A, L49A, D51A, R52A, L69A, Y239A, D241A,…

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. EW482 CMV-TO-ADAR2dd(E488Q, T501A)-PUF-9R-MARIAcomp(V241D, H243M) [D241C, M243Q] (FLP-IN)

   Plasmid

   #236128

   Purpose

   Plasmid encoding ADAR2dd attached to PUF-9R with deimmunizing and function-restoring mutations under control of CMV promoter with two TetR binding sites

   Depositor

   Xiaojing Gao

   Article

   Wolfsberg et al bioRxiv 2025.02.24.639918

   Insert

   ADAR2dd-PUF-9R (ADARB1 Synthetic, Human)
   

   Expression

   Mammalian

   Mutation

   E488Q, T501A in ADAR2dd; V241C, H243Q in PUF-9R

   Promoter

   CMV promoter with two TetR binding sites

   Available Since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. EW370 (PB) (ZNF35/ZNF250tarv3-6bp spacers)x4-H2B-BFP insul CMV-TO-ZNF35(5-8)-ZNF250(1-4)-deimlink-NZF

   Plasmid

   #236172

   Purpose

   PiggyBac-integrated plasmid containing hygromycin resistance gene, ZNF35/ZNF250-driven BFP, and ZNF35/ZNF250-NZF transcription factor

   Depositor

   Xiaojing Gao

   Article

   Wolfsberg et al bioRxiv 2025.02.24.639918

   Inserts

   ZNF35(5-8)-ZNF250(1-4)-deImmunLink-NZF
   H2B (H2BC11 Human)
   

   Tags

   mTagBFP2

   Expression

   Mammalian

   Promoter

   CMV and four ZNF35/ZNF250 sites

   Available Since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. EW489 CMV-TO-ZNF35(5-8)-ZNF250(1-4)-(GGS)x8[G2V G4W S6L G7L G10W G11D G13L G17M G19I G22M G23Q S24Q]-FKBP (FLP-IN)

   Plasmid

   #236147

   Purpose

   Plasmid encoding the ZNF35/ZNF250 zinc finger array attached by a deimmunized linker to FKBP1A, under control of CMV promoter with two TetR binding sites

   Depositor

   Xiaojing Gao

   Article

   Wolfsberg et al bioRxiv 2025.02.24.639918

   Insert

   ZNF35(5-8)-ZNF250(1-4)-deImmunLink-FKBP
   

   Expression

   Mammalian

   Promoter

   CMV promoter with two TetR binding sites

   Available Since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. EW267 CMV-TO-ADAR2dd(E488Q, T501A)-PUF-9R-MARIAcomp(V241D, H243M) (FLP-IN)

    Plasmid

    #236127

    Purpose

    Plasmid encoding ADAR2dd attached to PUF-9R with deimmunizing mutations under control of CMV promoter with two TetR binding sites

    Depositor

    Xiaojing Gao

    Article

    Wolfsberg et al bioRxiv 2025.02.24.639918

    Insert

    ADAR2dd-PUF-9R (ADARB1 Synthetic, Human)
    

    Expression

    Mammalian

    Mutation

    E488Q, T501A in ADAR2dd; V241D, H243M in PUF-9R

    Promoter

    CMV promoter with two TetR binding sites

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. EW372 (PB) (ZNF35/IKZF1tar-6bp spacers)x4-H2B-BFP insul CMV-TO-ZNF35(5-8)-IKZF1(2-4)-deimlink-NZF

    Plasmid

    #236174

    Purpose

    PiggyBac-integrated plasmid containing hygromycin resistance gene, ZNF35/IKZF1-driven BFP, and ZNF35/IKZF1-NZF transcription factor (with the IKZF1 portion containing ZFs 2-4 from that protein)

    Depositor

    Xiaojing Gao

    Article

    Wolfsberg et al bioRxiv 2025.02.24.639918

    Inserts

    ZNF35/IKZF1(2-4)-deimmunLink-NZF
    H2B (H2BC11 Human)
    

    Tags

    mTagBFP2

    Expression

    Mammalian

    Promoter

    CMV and four ZNF35/IKZF1 sites

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLAS2w.Phyg-CHST8 (3A)

    Plasmid

    #232651

    Purpose

    expresses CHST8 REP-to-AAA mutant in mammalian cells

    Depositor

    Chen-Yang Shen

    Article

    Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

    Insert

    CHST8 (3A) (CHST8 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    R258A, E259A, P260A

    Promoter

    CMV

    Available Since

    March 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pXJ-CD274-Nluc

    Plasmid

    #232658

    Purpose

    Nluc-tagged PD-L1 expresion in mammalian cells; lectin-captured luciferase reporter assay

    Depositor

    Chen-Yang Shen

    Article

    Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

    Insert

    CD274 (CD274 Human)
    

    Use

    Luciferase

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pXJ-Nluc-_(PLRSKK)-CA1-19

    Plasmid

    #232657

    Purpose

    Nluc-tagged modified CG_ in mammalian cells; lectin-captured luciferase reporter assay

    Depositor

    Chen-Yang Shen

    Article

    Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

    Insert

    CGA (CGA Human)
    

    Use

    Luciferase

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCM100_ PB5'IR-hU6-gRNA-CS1- CAG-tdTomato- PB3'IR

    Plasmid

    #229995

    Purpose

    PiggyBac sgRNA cloning plasmid with tdTomato reporter with a capture sequence (cs1)

    Depositor

    Christian Mayer

    Article

    Dvoretskova et al Nat Neurosci. 2024 May;27(5):862-872. do

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    NotI site for NEBuilder HiFi DNA Assembly with ss…

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. FLAG-MKI-MUT-P2A-GFP

    Plasmid

    #229406

    Purpose

    Lentiviral or overpexression of cDNA

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    MKI-MUT-P2A-GFP
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    interaction mutant L109G; L143G (Control mutant)

    Promoter

    EFS

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FLAG-MKI-P2A-GFP-DOX-N

    Plasmid

    #229407

    Purpose

    Lentiviral or overpexression of cDNA

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    MKI-P2A-GFP
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FLAG-MKI-P2A-GFP

    Plasmid

    #229405

    Purpose

    Lentiviral or overpexression of cDNA

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    MKI-P2A-GFP
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HA-MOB1A

    Plasmid

    #229427

    Purpose

    Lentiviral or overpexression of cDNA

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    MOB1A (MOB1A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HA-MOB1B

    Plasmid

    #229428

    Purpose

    Lentiviral or overpexression of cDNA

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    MOB1B (MOB1B Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge