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Plasmid#120728PurposeExpression of a Flag-tagged version of DDX11 KAE mutant in mammalian cellsDepositorInsertDDX11 KAE mutant (DDX11 Human)
UseTags3x FLAGExpressionMammalianMutationSubstitution of E201 and Y202 with K and APromoterCMVAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-DDX11-Flag-KAK
Plasmid#120729PurposeExpression of a Flag-tagged version of DDX11 KAK mutant in mammalian cellsDepositorInsertDDX11 KAK mutant (DDX11 Human)
UseTags3x FLAGExpressionMammalianMutationSubstitution of E201, Y202 and E203 with K, A and…PromoterCMVAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Enhancer-ZC3H12D_PromoterMUT
Plasmid#153068PurposeZC3H12D promoter region carrying mutations in BHLHE40 binding sites, cloned upstream of luciferase reporterDepositorInsertZC3H12D promoter region mutated in BHLHE40 binding sites (ZC3H12D Human)
UseLuciferaseTagsExpressionMammalianMutationMutagenesis to disrupt 3 BHLHE40 binding sites. N…PromoterNoneAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF-casBCDE(+6)
Plasmid#89728PurposeExpresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a longer version of the wt spacer (+6) extended by 6 nucleotides at the leader-distal end.DepositorInsertCRISPR array
UseTagsExpressionBacterialMutationDerivative CRISPR array containing a longer versi…PromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1_GST-EGFP-ATG13_IDR (191-205aa)
Plasmid#223791PurposeExpression of recombinant protein for purificationDepositorInsertATG13 IDR (ATG13 Human)
UseTagsGST-EGFPExpressionBacterialMutationaa 191-205 onlyPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1_GST-EGFP-ATG13_IDR (191-230aa)
Plasmid#223792PurposeExpression of recombinant protein for purificationDepositorInsertATG13 IDR (ATG13 Human)
UseTagsGST-EGFPExpressionBacterialMutationaa 191-230 onlyPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1_GST-EGFP-ATG13_IDR (206-230aa)
Plasmid#223793PurposeExpression of recombinant protein for purificationDepositorInsertATG13 IDR (ATG13 Human)
UseTagsGST-EGFPExpressionBacterialMutationaa 206-230 onlyPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGenlenti_ATG13-delta-206-230
Plasmid#223774PurposeStable expression of protein in mammalian cell culture, rescue experimentsDepositorInsertATG13 (ATG13 Human)
UseLentiviralTagsExpressionMutationaa 206-230 deleted (expresses 1-205, 231-517)PromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-Dual_BNIP3(1-158aa)-THROMBIN-GST (W18A/L21A; deltaLIR)
Plasmid#223778PurposeExpression of recombinant protein for purificationDepositorInsertBNIP3 soluble part (BNIP3 Human)
UseTagsThrombin cleavage-GSTExpressionInsectMutationW18A/L21A; deletion of LIRPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1_GST-EGFP-ATG13_IDR_delta191-230 (231-517aa)
Plasmid#223785PurposeExpression of recombinant protein for purificationDepositorInsertATG13 IDR (ATG13 Human)
UseTagsGST-EGFPExpressionBacterialMutationaa 231-517 onlyPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1_GST-EGFP-ATG13_IDR_delta191-204 (205-517aa)
Plasmid#223786PurposeExpression of recombinant protein for purificationDepositorInsertATG13 IDR (ATG13 Human)
UseTagsGST-EGFPExpressionBacterialMutationaa 205-517 onlyPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1_GST-EGFP-ATG13_IDR_delta206-230 (191-205_231-517aa)
Plasmid#223787PurposeExpression of recombinant protein for purificationDepositorInsertATG13 IDR (ATG13 Human)
UseTagsGST-EGFPExpressionBacterialMutationexpresses aa 191-517, with 206-230 deletedPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGenLenti V5-NIX (E72A/L75A/D77A/E81A; deltaWIPI2)
Plasmid#223789PurposeStable expression of protein in mammalian cell cultureDepositorInsertNIX (BNIP3L) (BNIP3L Human)
UseLentiviralTagsV5ExpressionMutationE72A/L75A/D77A/E81A; disrupts WIPI2 bindingPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_6xHis-TEV-mCherry-ATG13 IDR (191-517aa)
Plasmid#223762PurposeExpression of recombinant protein for purificationDepositorInsertATG13 IDR (ATG13 Human)
UseTags6xHis-TEV-mCherryExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-Dual_BNIP3(1-158aa)-EGFP-TEV-GST (W18A/L21A; deltaLIR)
Plasmid#223766PurposeExpression of recombinant protein for purificationDepositorInsertBNIP3 soluble part (BNIP3 Human)
UseTagsEGFP-TEV-GSTExpressionInsectMutationW18A/L21A; deletion of LIRPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_NIX(1-182aa)-THROMBIN-GST (W36A/L39A; deltaLIR)
Plasmid#223738PurposeExpression of recombinant protein for purificationDepositorInsertNIX (BNIP3L) soluble part (BNIP3L Human)
UseTagsThrombin cleavage-GSTExpressionBacterialMutationW36A/L39A; deletion of LIRPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_FUNDC1(1-50aa)-THROMBIN-GST (Y18A/L21A; deltaLIR)
Plasmid#223739PurposeExpression of recombinant protein for purificationDepositorInsertFUNDC1 soluble part, mutant (FUNDC1 Human)
UseTagsThrombin cleavage-GSTExpressionBacterialMutationY18A/L21A; deletion of LIRPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_NIX(1-182aa)-EGFP-TEV-GST (W36A/L39A; deltaLIR)
Plasmid#223748PurposeExpression of recombinant protein for purificationDepositorInsertNIX (BNIP3L) soluble part (BNIP3L Human)
UseTagsEGFP-TEV-GSTExpressionBacterialMutationW36A/L39A; deletion of LIRPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr2-EREG-ScNeo
Plasmid#209909PurposeTo monitor the status of Epiregulin, the plasmid encodes a recombinant Epiregulin fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent proteinDepositorInsertEREG-ScNeo (EREG Human)
UseTagsGGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…ExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr2-AREG-ScNeo
Plasmid#209910PurposeTo monitor the status of Amphiregulin, the plasmid encodes a recombinant AREG fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent proteinDepositorInsertAREG-ScNeo (AREG Human)
UseTagsmNeonGreen and mScarletExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only