We narrowed to 5,822 results for: pcas

1. pCA528 SPASTIN , L177D, residues 108-200

   Plasmid

   #180634

   Purpose

   Bacterial expression for SPASTIN MIT, residues 108-200. Has L177D mutation. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-13.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   SPASTIN
   

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 108-200, L177D

   Promoter

   T7

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCA528 CHMP4B, residues 156-224, no Cys

   Plasmid

   #180641

   Purpose

   Bacterial expression for CHMP4B C-terminal tail, residues 156-224. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-140

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   CHMP4B (CHMP4B Human)
   

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 156-224

   Promoter

   T7

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCA528 CHMP4C, residues 156-233, no Cys

   Plasmid

   #180642

   Purpose

   Bacterial expression for CHMP4C C-terminal tail, residues 156-233. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-14.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   CHMP4C (CHMP4C Human)
   

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   residues 316-366

   Promoter

   T7

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCA528 IST1, residues 315-360, no Cys

   Plasmid

   #180643

   Purpose

   Bacterial expression of IST1 C-terminal tail, residues 315-360. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-99.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   IST1 (IST1 Human)
   

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   residues 315-360

   Promoter

   T7

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCA528 RPS6KC1 MIT, residues 235-316

   Plasmid

   #180622

   Purpose

   Bacterial expression for RPS6KC1 MIT domain, residues 235-316. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-87.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   RPS6KC1 (RPS6KC1 Human)
   

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 235-316

   Promoter

   T7

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-DIO-inv[roxSTOProx-ZsGreen-bGH]

   Plasmid

   #196883

   Purpose

   Dual Cre/Dre-dependent expression of ZsGreen. Used as Cre-Dre cotransfection reporter

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   roxSTOProx-ZsGreen-bGH
   

   Use

   Cre/Lox; Dre/rox

   Expression

   Mammalian

   Promoter

   Quimeric CAG

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCA19-HSPB1 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)

   Plasmid

   #199450

   Purpose

   CRISPR donor plasmid to insert TriTag (mTagBFP harbors 12XMS2 in its intron) into the C-terminus of human HSPB1 gene

   Depositor

   Baohui Chen

   Article

   Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

   Insert

   HDR donor template
   

   Use

   CRISPR

   Available Since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCA22-SEC61B 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)

   Plasmid

   #199453

   Purpose

   CRISPR donor plasmid to insert TriTag (mTagBFP) into the C-terminus of human SEC61B gene

   Depositor

   Baohui Chen

   Article

   Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

   Insert

   HDR donor template
   

   Use

   CRISPR

   Available Since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCA31-pCS2-CMV-stdPCP-p65-HSF1

   Plasmid

   #199462

   Purpose

   Expression of stdPCP-PH in zebrafish embryos

   Depositor

   Baohui Chen

   Article

   Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

   Insert

   stdPCP-PH
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCA30-pCS2-CMV-stdMCP-p65-HSF1

    Plasmid

    #199461

    Purpose

    Expression of stdMCP-PH in zebrafish embryos

    Depositor

    Baohui Chen

    Article

    Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

    Insert

    stdMCP-PH
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCA21-HSPB1 5'HA-NarTag (12XPP7)-3'HA (HDR donor)

    Plasmid

    #199452

    Purpose

    CRISPR donor plasmid to insert NarTag (mTagBFP harbors 12XPP7 in its intron) into the C-terminus of human HSPB1 gene

    Depositor

    Baohui Chen

    Article

    Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

    Insert

    HDR donor template
    

    Use

    CRISPR

    Available Since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCA29-pCS2-CMV-Zebrafish NarTag (eGFP)

    Plasmid

    #199460

    Purpose

    Expression of Zebrafish NarTag (eGFP harbors 9XMS2 in its intron) in zebrafish embryos

    Depositor

    Baohui Chen

    Article

    Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

    Insert

    Zebrafish NarTag
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAG-FLAG-IRES-Blasticidin-hOCT4 S236D

    Plasmid

    #198765

    Purpose

    expression of hOCT4 mutants

    Depositor

    Hyonchol Jang

    Article

    Kim et al Cancers (Basel). 2020 Sep 11;12(9):2601.

    Insert

    hOCT4 236D (POU5F1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S236D

    Available Since

    April 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX335_U6-Chimeric_BB-CBh-hSpCas9n(D10A)_SOX17_Ex2_KI

    Plasmid

    #195502

    Purpose

    Cas9 nickase expression vector bearing a sgRNA targeting Exon 2 of human SOX17

    Depositor

    Alexander Meissner

    Article

    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

    Insert

    sgRNA (SOX17 Human)
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)_g1_CASH-1

    Plasmid

    #188975

    Purpose

    A human codon-optimized SpCas9 nickase and chimeric g1 guide RNA expression plasmid.

    Depositor

    Sangdun Choi

    Article

    Javaid et al Stem Cells Int. 2022 Sep 22;2022:4537335

    Insert

    g1 guide RNA
    

    Expression

    Mammalian

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)_g2_CASH-1

    Plasmid

    #188976

    Purpose

    A human codon-optimized SpCas9 nickase and chimeric g2 guide RNA expression plasmid.

    Depositor

    Sangdun Choi

    Article

    Javaid et al Stem Cells Int. 2022 Sep 22;2022:4537335

    Insert

    g2 guide RNA
    

    Expression

    Mammalian

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAG-MBP-Foldon-ATG9 (801-839)

    Plasmid

    #188089

    Purpose

    To make ATG9 C-terminal tail trimer for expression in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG9 (ATG9A Human)
    

    Tags

    MBP tag

    Expression

    Mammalian

    Mutation

    ATG9 (801-839)

    Promoter

    CMV

    Available Since

    Aug. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-MBP-Foldon-ATG9 (723-839)

    Plasmid

    #188088

    Purpose

    To make ATG9 C-terminal tail trimer for expression in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG9 (ATG9A Human)
    

    Tags

    MBP tag

    Expression

    Mammalian

    Mutation

    ATG9 (723-839)

    Promoter

    CMV

    Available Since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG-MBP-Foldon-ATG9 (828-839)

    Plasmid

    #188090

    Purpose

    To make ATG9 C-terminal tail trimer for expression in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG9 (ATG9A Human)
    

    Tags

    MBP tag

    Expression

    Mammalian

    Mutation

    ATG9 (828-839)

    Promoter

    CMV

    Available Since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. BPK880-pCAG-DmrC-NLS-3xFLAG-VP64

    Plasmid

    #136912

    Purpose

    DmrC with VP64 fused to it's C-terminus; Mammalian expression vector

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

    Insert

    pCAG-DmrC-NLS-3xFLAG-VP64
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Chicken Beta Actin

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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