We narrowed to 54,462 results for: plasmid

1. CaMKII-somBiPOLES-ChroME2f-mCerulean

   Plasmid

   #192581

   Purpose

   AAV-vector for bidirectional optogenetic manipulation of excitatory neurons with blue shifted excitation spectrum

   Depositor

   Simon Wiegert

   Insert

   somBiPOLES
   

   Use

   AAV

   Tags

   soma-targeting motif from Kv2.1 channel

   Expression

   Mammalian

   Promoter

   CaMKIIa(0.4)

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSCM167.2-Venus-VF-hSFRP1

   Plasmid

   #189541

   Purpose

   Protein expression of SFRP1 in mammalian cells.

   Depositor

   Haian Fu

   Insert

   secreted frizzled related protein 1 (SFRP1 Human)
   

   Tags

   VF

   Expression

   Mammalian

   Available Since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAIO-Ef1a-PE2-GFP:KCNQ2-C201R_pp

   Plasmid

   #185061

   Purpose

   Ef1a driven PE2 with pegRNA for editing C201R mutation (including silent PAM mutation) in KCNQ2. See Addgene plasmid #184445

   Depositor

   Bobby Koeleman

   Insert

   U6:pegRNA:scaffold:PBS+RT template
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.2-V5-DEST-hSFRP1-GST

   Plasmid

   #189543

   Purpose

   Protein expression of SFRP1 in mammalian cells.

   Depositor

   Haian Fu

   Insert

   secreted frizzled related protein 1 (SFRP1 Human)
   

   Tags

   GST

   Expression

   Mammalian

   Available Since

   Oct. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOTTC1584 - pscAAV CMV-IE secreted EGFP-THPKTEL WPRE

   Plasmid

   #188539

   Purpose

   An AAV packaging vector that expresses secreted C-CDNF under control of the EGFP promoter.

   Depositor

   Brandon Harvey

   Insert

   secreted EGFP
   

   Use

   AAV

   Tags

   CDNF(1-28) and THPKTEL WPRE

   Promoter

   CMV-IE

   Available Since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Akaluc VLP

   Plasmid

   #182212

   Purpose

   YIp for expressing MPyV deltaVP1 (GAL1 promoter) and Akaluc tagged with VP2C (GAL10 promoter).

   Depositor

   Claudia Vickers

   Inserts

   VP2C-Akaluc
   Murine polyomavirus deltaVP1 (NLS deletion mutant)
   

   Use

   Synthetic Biology; Tem imaging

   Expression

   Yeast

   Promoter

   GAL1 and GAL10

   Available Since

   March 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. SDCBPA-c020

   Plasmid

   #175484

   Purpose

   Protein expression in bacterial cells. PDZ1 and PDZ2 domains, G111-V288.

   Depositor

   Opher Gileadi

   Insert

   SDCBP (SDCBP Human)
   

   Tags

   His6-TEV

   Expression

   Bacterial

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV V5-Eva1b

   Plasmid

   #175158

   Purpose

   Lentiviral expression of V5-tagged mouse Eva1b

   Depositor

   Larry Gerace

   Insert

   Eva1b (Eva1b Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Jan. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV Cenpv-V5

   Plasmid

   #175125

   Purpose

   Lentiviral expression of mouse Cenpv-V5

   Depositor

   Larry Gerace

   Insert

   Cenpv (Cenpv Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. L-SACSIN-74

    Plasmid

    #166526

    Purpose

    scFv of a human scaffold targeting Sacsin, DnaJ homolog subfamily C member 29. Antigen coverage aa 84-339 of 4579

    Depositor

    Susanne Gräslund

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, Sortag, His6 and OmpA leader sequence

    Expression

    Bacterial

    Promoter

    LacZ

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. O-FHL1-1

    Plasmid

    #166543

    Purpose

    scFv of a human scaffold targeting Four and a half LIM domains protein 1. Antigen coverage aa 1-280 aa, full-length

    Depositor

    Susanne Gräslund

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Promoter

    LacZ

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. C-BRPF3-8

    Plasmid

    #166516

    Purpose

    scFv of a human scaffold targeting Bromodomain and PHD finger-containing protein 3. Antigen coverage aa 1056-1195 of 1205, PWWP domain.

    Depositor

    Susanne Gräslund

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Promoter

    LacZ

    Available Since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNR2F1-donor

    Plasmid

    #112388

    Purpose

    CRISPR donor plasmid to tag human transcription factor NR2F1 with GFP

    Depositor

    Kevin White

    Insert

    NR2F1 homology arms flanking EGFP-IRES-Neo cassette (NR2F1 Human)
    

    Use

    CRISPR

    Mutation

    rs974916569

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pZNF250-donor

    Plasmid

    #112383

    Purpose

    CRISPR donor plasmid to tag human transcription factor ZNF250 with GFP

    Depositor

    Kevin White

    Insert

    ZNF250 homology arms flanking EGFP-IRES-Neo cassette (ZNF250 Human)
    

    Use

    CRISPR

    Mutation

    hg38:8:144882271:C>G,hg38:8:144882273:T>C

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pZNF140-donor

    Plasmid

    #112363

    Purpose

    CRISPR donor plasmid to tag human transcription factor ZNF140 with GFP

    Depositor

    Kevin White

    Insert

    ZNF140 homology arms flanking EGFP-IRES-Neo cassette (ZNF140 Human)
    

    Use

    CRISPR

    Mutation

    hg38:12:133105834:A>G, hg38:12:133105852:A>G

    Available Since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pZNF639-donor

    Plasmid

    #112345

    Purpose

    CRISPR donor plasmid to tag human transcription factor ZNF639 with GFP

    Depositor

    Kevin White

    Insert

    ZNF639 homology arms flanking EGFP-IRES-Neo cassette (ZNF639 Human)
    

    Use

    CRISPR

    Mutation

    hg38:3:179333542:G>T,rs978050614

    Available Since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTBPL1-donor

    Plasmid

    #112316

    Purpose

    CRISPR donor plasmid to tag human transcription factor TBPL1 with GFP

    Depositor

    Kevin White

    Insert

    TBPL1 homology arms flanking EGFP-IRES-Neo cassette (TBPL1 Human)
    

    Use

    CRISPR

    Mutation

    rs35776966, hg38:6:133987646:GTGTAT>-

    Available Since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. PKN2 (PRKCL2A-c038)

    Plasmid

    #110265

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PRKCL2 (PKN2 Human)
    

    Tags

    His6-ZBasic-TEV

    Expression

    Insect

    Promoter

    polyhedrin promoter

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-Flag-Hsp40-S149A/S151A

    Plasmid

    #84998

    Purpose

    Hsp40/DnaJB1 expression plasmid

    Depositor

    Ugo Moens

    Insert

    Hsp40 (DNAJB1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Serine 149 and 151 mutated to Alanine

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

20. KCTD17

    Plasmid

    #74661

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    KCTD17A (KCTD17 Human)
    

    Tags

    His-6-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only