We narrowed to 9,183 results for: try;

1. pJCC_076 AsCas12a R1226A

   Plasmid

   #171669

   Purpose

   For bacterial expression of AsCas12a R1226A (RuvC-inhibiting mutation) with an N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

   Insert

   AsCas12a R1226A
   

   Use

   Tags

   10X His, TEV protease cleavage site, and maltose-…

   Expression

   Bacterial

   Mutation

   changed arginine 1226 to alanine

   Promoter

   Available since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSFDuet1-His-TEV-OTC_Ub

   Plasmid

   #172134

   Purpose

   Scaffold design for studying ubiquitin interacting proteins by cryo-EM.

   Depositor

   Shang-Te Danny Hsu

   Article

   Chiu et al Biochemistry. 2021 Apr 13;60(14):1075-10

   Insert

   E. coli ornithine transcarbamylase (OTC), fused to human ubiquitin
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSecTag-T210A-ATX

   Plasmid

   #17840

   Depositor

   Steven Rosen

   Article

   Kanda et al Nat Immunol. 2008 Apr . 9(4):415-23.

   Insert

   T210A-ATX (ENPP2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 29, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA HisB TO rFez1

   Plasmid

   #17385

   Depositor

   Stephen Goff

   Article

   Naghavi et al Genes Dev. 2005 May 1. 19(9):1105-15.

   Insert

   FEZ1 (Fez1 Rat)
   

   Use

   Tags

   his and myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 1, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pENTR221-NFAT5

   Plasmid

   #79506

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Human NFAT5 CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   NFAT5 (NFAT5 Human)
   

   Use

   Multisite gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   no Promoter

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMX-hTAF7

   Plasmid

   #44338

   Depositor

   Marc Timmers

   Article

   Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

   Insert

   TAF7 (TAF7 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   May 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. hDAGLb-V5

   Plasmid

   #87677

   Purpose

   Expresses human DAGLb protein with intracellular V5 tag located on the C-terminal

   Depositor

   Pat Doherty

   Article

   Singh et al Biochemistry. 2016 May 17;55(19):2713-21

   Insert

   Human Diacylglycerol Lipase Beta (DAGLB Human)
   

   Use

   Tags

   V5 tag

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCK081 desmaMCS:minpromCerulean,exorh:mCherry

   Plasmid

   #200014

   Purpose

   Expression construct with desmin a regulatory elements, mouse beta-globin minimal promoter mCerulean, exorh:mCherry

   Depositor

   Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Type

   Empty backbone

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR1A hu_ncOGT WT

   Plasmid

   #154281

   Purpose

   Entry vector encoding non-tagged O-GlcNAc Transferase

   Depositor

   Suzanne Walker

   Article

   Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Gateway entry vector

   Tags

   2x FLAG and mKate2

   Expression

   Mutation

   WT OGT

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pirespuro2-CD86-mEos2

    Plasmid

    #98284

    Purpose

    mammalian expression of CD86-mEos2

    Depositor

    Mike Heilemann

    Article

    Fricke et al Sci Rep. 2015 Sep 11;5:14072. doi: 10.10

    Insert

    CD86 (CD86 Human)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMX-hTAF10

    Plasmid

    #44340

    Depositor

    Marc Timmers

    Article

    Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

    Insert

    TAF10 (TAF10 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    July 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENTR223-BALF5

    Plasmid

    #198577

    Purpose

    Gateway Entry Clone

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BALF5 (BALF5 Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    None (wildtype)

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR223-BALF2

    Plasmid

    #198578

    Purpose

    Gateway Entry Clone

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BALF2 (BALF2 Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    None (wildtype)

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRK5-HA-YFP-PRAS40

    Plasmid

    #73389

    Purpose

    Expresses HA and YFP tagged PRAS40

    Depositor

    Jie Chen, Taekjip Ha

    Article

    Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

    Insert

    AKT1 Substrate 1 (Proline-Rich) (AKT1S1 Human)
    

    Use

    Tags

    HA and YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pZA8

    Plasmid

    #158486

    Purpose

    Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E/D481V with STOP codon

    Depositor

    Sophien Kamoun

    Article

    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

    Insert

    ZAR1
    

    Use

    Golden gate entry vector

    Tags

    Expression

    Mutation

    L17E, D481V, STOP codon

    Promoter

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. SpyTag003-OspC

    Plasmid

    #159998

    Purpose

    Expresses Borrelia burgdorferi OspC (20-209) C129S with N-terminal SpyTag003 fusion in bacterial cells

    Depositor

    Mark Howarth

    Article

    Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

    Insert

    SpyTag003-OspC (20-209) C129S
    

    Use

    Tags

    His6-tag and Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    C129S mutation improves expression level.

    Promoter

    T7

    Available since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. MAC-SARS-CoV-2 NSP6

    Plasmid

    #158357

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    SARS-CoV-2 NSP6 (ORF1ab )
    

    Use

    Tags

    MAC-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. IA001: pMVP (L3-L2) 3x HA epitope tag+ polyA

    Plasmid

    #121750

    Purpose

    pMVP L3-L2 entry plasmid, contains 3x HA epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    3x HA epitope tag + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYZ172

    Plasmid

    #98409

    Purpose

    Donor DNA plasmid to introduce lys9 deletion in S. pombe. Linearization with Not1 digestion.

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Inserts

    upstream homologous region to delete lys9 in genome by recombination
    downstream homologous region to delete lys9 in genome by recombination
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. MAC-SARS-CoV-2 N

    Plasmid

    #158370

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    SARS-CoV-2 N (N )
    

    Use

    Tags

    MAC-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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