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Plasmid#217586PurposeDestination vector with kanamycin resistance and p15A origin of replication. Carries G-H CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only
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DVK_p15A_EF
Plasmid#217584PurposeDestination vector with kanamycin resistance and p15A origin of replication. Carries E-F CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDESTpK7WG2-RPS5Ap-NLS-OleGFP
Plasmid#193370PurposenTALEN or nTALECD vector assembly, Destination vector for plants to express ORFs from entry clone with NLS under control of the RPS5A promoter and HSP terminator.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
INT_SS9sp
Plasmid#212145PurposeEffector plasmid for for operon integration into the genome (Safe Site 9) using CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, VchTnsA, VchTnsB, VchTnsC
UseTagsExpressionBacterialMutationPromoterpJ23119Available sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
spoT-
Plasmid#100913Purposedeletes synpcc7942_1377 (spoT)DepositorInsertKanamycin casette (in place of spoT gene) flanked by S. elongatus sequence
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDonor_ShCAST_kanR
Plasmid#127924PurposeKanamycin resistance donor for ShCASTDepositorInsertKanR, R6K origin of replication
UseTagsExpressionMutationPromoterAvailable sinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
RepX_pET28a(+)
Plasmid#172199PurposeExpresses 6xHis-tagged RepX in E. coli BL21(DE3), kanamycin resistance, optimal growth at 20 degrees.DepositorInsertRepX superhelicase
UseTags6xHis tagExpressionBacterialMutationC18L, C43S, C167V, S400C, C612APromoterAvailable sinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDD131
Plasmid#119886PurposeOptimized aTc inducible pTet promoter for Rhodococcus opacus expressing mCherry on pAL5000/pMB1 backbone with kanamycin resistance.DepositorInsertpTet_mCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
YFP-GID1
Plasmid#37305DepositorInsertSee comments
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDR-GW FLII12Pglu-700μδ6
Plasmid#28002DepositorInsertFLII12Pglu-700μδ6
UseTagseCFP and eYFPExpressionYeastMutationPromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pKESK22
Plasmid#64857Purposeexpression vector, low to medium copy number, tac promoter lacI, kanamycin resistance, p15A origin of replicationDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterPtacAvailable sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDR-GW AT1.03YEMK
Plasmid#28004DepositorInsertAT1.03YEMK
UseTagsmVenus and mseCFPExpressionYeastMutationM60Y, G131E, K132M, 133KPromoterAvailable sinceSept. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFCyR2
Plasmid#167513PurposeBroad-Host range expression vector, RSF1010 origin, CymRAM repressor, PCymRC promoter, mRFP, Kanamycin Resistance Marker.DepositorInsertCymRAM-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPCymRCAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBTG2
Plasmid#149475PurposeBroad-Host range expression vector, pBBR origin, TetR repressor, PTetA promoter, GFPmut3b, Kanamycin Resistance Marker.DepositorInsertTetR-GFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPTetAAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDD87
Plasmid#119892PurposeThe pLPD03031 promoter from Rhodococcus opacus PD630 expressing GFP+ on the pAL5000/pMB1 backbone with kanamycin resistance.DepositorInsertpLPD03031_GFP+
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBNG2
Plasmid#149473PurposeBroad-Host range expression vector, pBBR origin, NahR(AM) repressor, PSalTTC promoter, GFPmut3b, Kanamycin Resistance Marker.DepositorInsertNahR(AM)-GFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPSalTTCAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBSV2_2
Plasmid#118226PurposeEmpty E. coli - B. burgdorferi shuttle vector; kanamycin resistantDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW AKAR3-EV
Plasmid#182533PurposeExpresses the PKA FRET sensor AKAR3-EV in budding yeastDepositorInsertAKAR3-EV
UseTagsExpressionYeastMutationPromoterPMA1Available sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBCiG2
Plasmid#149470PurposeBroad-Host range expression vector, pBBR origin, CinR(AM) activator, PCin promoter, GFPmut3b, Kanamycin Resistance Marker.DepositorInsertCinR(AM)-GFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPCinAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAd NeuroD-I-nGFP
Plasmid#19414DepositorInsertneurogenic differentiation 1 (Neurod1 Mouse)
UseAdenoviral; Entry vectorTagsGFP and IRESExpressionMutationPromoterAvailable sinceNov. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAd 2B Ngn3-I-nGFP
Plasmid#19410DepositorInsertneurogenin 3 (Neurog3 Mouse)
UseAdenoviral; Entry vectorTagsGFP and IRESExpressionMutationPromoterAvailable sinceNov. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRGD-KmR
Plasmid#74108PurposePlasmid containing kanamycin resistance gene cassette flanked by multiple restriction sitesDepositorInsertaphII
UseBacterial vector based on pmb1 repliconTagsExpressionMutationPromoterAvailable sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pProm1_BCD1-GFP
Plasmid#80649PurposePromoter1 with BCD1-gfpDepositorInsertGFP
UseP15a origin, constitutive promoter, kanrTagsExpressionMutationNONEPromoterconstitutive promoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
MBP-TEV-Gly-RadA C46
Plasmid#128736PurposeExpresses PhoRadA intein split at residue 46 from the C-terminalDepositorInsertRadA
UseTagsHisx6-MBPExpressionBacterialMutationPromoterT7Available sinceAug. 12, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFCiR2
Plasmid#167512PurposeBroad-Host range expression vector, RSF1010 origin, CinRAM activator, PCin promoter, mRFP, Kanamycin Resistance Marker.DepositorInsertCinRAM-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPCinAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBLxG2
Plasmid#149472PurposeBroad-Host range expression vector, pBBR origin, LuxR activator, PLuxB promoter, GFPmut3b, Kanamycin Resistance Marker.DepositorInsertLuxR-GFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLuxBAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDR-GW FLIPglu-170nΔ13V
Plasmid#27999DepositorInsertFLIPglu-170nΔ13V
UseTagseCFP and eYFPExpressionYeastMutationWT binding protein (no F16A)PromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDR-GW FLIPglu-30μΔ13V
Plasmid#27998DepositorInsertFLIPglu-30μΔ13V
UseTagseCFP and eYFPExpressionYeastMutationD154A in mglBPromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDR-GW FLIPglu-2µΔ13V
Plasmid#28000DepositorInsertFLIPglu-2µΔ13V
UseTagseCFP and eYFPExpressionYeastMutationH152APromoterAvailable sinceJune 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDR-GW FLIPglu-600μΔ13V
Plasmid#28001DepositorInsertFLIPglu-600μΔ13V
UseTagseCFP and eYFPExpressionYeastMutationF16A in mglBPromoterAvailable sinceMarch 31, 2011AvailabilityAcademic Institutions and Nonprofits only