-
Plasmid#176562PurposeExpression of TagRFP657 for auxotrophic selection in the absence of uracilDepositorInsertTagRFP657
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH-Sapphire
Plasmid#176566PurposeExpression of Sapphire for auxotrophic selection in the absence of lysineDepositorInsertyETSapphire
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU-Sapphire
Plasmid#176567PurposeExpression of Sapphire for auxotrophic selection in the absence of uracilDepositorInsertyETSapphire
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pM-Sapphire
Plasmid#176569PurposeExpression of Sapphire for auxotrophic selection in the absence of methionineDepositorInsertyETSapphire
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
TaALMT1-upGFP 5'
Plasmid#117891Purposeprotein expressionDepositorInsertTaALMT1-upGFP 5'
UseTagseGFPExpressionYeastMutationPromoterAOXAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
TaALMT1-upGFP 3'
Plasmid#117893Purposeprotein expressionDepositorInsertTaALMT1-upGFP 3'
UseTagseGFPExpressionYeastMutationPromoterAOXAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYSD085
Plasmid#212922PurposeEncodes the HA-tagged 649 Stalk yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsert649 Stalk
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB4906
Plasmid#106150PurposeEasyCloneYALI system-based yeast integrative vector expressing Cas9 carrying hygromycin resistance marker, integration into Yarrowia lipolytica chromosomal location IntB, amp resistanceDepositorInsertCas9
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLAREG
Plasmid#73044PurposeInducible expression of EGFP in yeast. Has dual-mode promoter with activation and repression capability for tuning gene expression in yeast.DepositorInsertsEGFP
LacI
UseTagsExpressionYeastMutationPromoterGAL1 promoter and hybrid dual-mode promoter-see c…Available sinceFeb. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAH243
Plasmid#121147PurposeExpression of both nmt41p-cas9 and rrk1p-gRNA (ura4 marker) for CRISPR genome editing in fission yeastDepositorInsertshumanized Streptococcus pyogenes Cas9
gRNA expression module
UseCRISPRTagsFlagExpressionYeastMutationPromoterS.pombe rrk1 and nmt41Available sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBAC690-TEF
Plasmid#160432PurposeExpresses eGFP under control of the yeast TEF2 promoterDepositorInsertS. cerevisiae TEF2 promoter
UseTagsExpressionYeastMutationPromoterS. cerevisiae TEF2Available sinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCY 3090-07
Plasmid#36232DepositorInsertStreptoalloteichus hindustanus bleomycin (Sh ble)
UseYeast cassette for pcr and homologous recombinati…TagsyEpolylinker-mCherry-ZeocinExpressionMutationPromoterAvailable sinceMay 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCY 3010-07
Plasmid#36212DepositorInsertStreptoalloteichus hindustanus bleomycin (Sh ble)
UseYeast cassette for pcr and homologous recombinati…TagsyEpolylinker-Cerulean-ZeocinExpressionMutationPromoterAvailable sinceJune 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFa6-TID-FRB-NLS-3HA-KanMX6
Plasmid#228022PurposeTo integrate TID-FRB-NLS fusion at yeast promoter; yeast BioID with KanMX6 markerDepositorInsertTurboID-FRB
UseTagsHA tagExpressionYeastMutationPromoterAvailable sinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFa6-TID-FRB-NLS-3HA-HIS3MX6
Plasmid#228023PurposeTo integrate TID-FRB-NLS fusion at yeast promoter; yeast BioID with HIS3MX6 markerDepositorInsertTurboID-FRB
UseTagsHA tagExpressionYeastMutationPromoterAvailable sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFa6-TID-FKBP-NLS-3HA-HISMX6
Plasmid#228027PurposeTo integrate TID-FKBP-NLS fusion at yeast promoter; yeast BioID with HISMX6 markerDepositorInsertTurboID-FKBP
UseTagsHA tagExpressionYeastMutationPromoterAvailable sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFa6-TID-FKBP-3HA-KanMX6
Plasmid#228024PurposeTo integrate TID-FKBP fusion at yeast promoter; yeast BioID with KanMX6 markerDepositorInsertTurboID-FKBP
UseTagsHA tagExpressionYeastMutationPromoterAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFa6-TID-FKBP-NLS-3HA-KanMX6
Plasmid#228026PurposeTo integrate TID-FKBP-NLS fusion at yeast promoter; yeast BioID with KanMX6 markerDepositorInsertTurboID-FKBP
UseTagsHA tagExpressionYeastMutationPromoterAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTH862-CEN-slowmaxRLuc/minCFLuc
Plasmid#115371PurposeExpresses translation initiation-controlled Renilla luciferase and translation elongation-controlled firefly luciferaseDepositorInsertsFirefly Luciferase (codon minimised)
Gcn4 5'-UTR + Renilla luciferase (codon optimised)
UseTagsExpressionYeastMutationAll codons have been changed for the most favoura…PromoterADH1 and TDH3Available sinceJune 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
FRP322
Plasmid#159303PurposeYeast tagging vector to create gene fusion with yeast codon optimized CitrineA206K under KanMX selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationV2del, S3del, F64L, A206K, L231HPromoterAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
-
-
-
pTIN7m
Plasmid#162886PurposeOverexpression vector for the fission yeast Schizosaccharomyces pombe (lower strength)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUG6SP-tTA'
Plasmid#41030DepositorInserttTA' (tetR'-VP16)
UseTagsExpressionMutationPromoterAvailable sinceDec. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUG6SP-tTA
Plasmid#41029DepositorInserttTA (tetR-VP16)
UseTagsExpressionMutationPromoterAvailable sinceDec. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGBK CUP1 mGrape3
Plasmid#170265PurposeYeast two-hybrid vector using a copper inducible CUP1 promoter, expressing mGrape3DepositorArticleInsertmomeric Grape 3
UseTagsGAL4 DNA Binding Domain and MycExpressionBacterial and YeastMutationPromoterCUP1Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBK CUP1 EGFP
Plasmid#170210PurposeYeast two-hybrid vector using a copper inducible CUP1 promoter, expressing EGFPDepositorArticleInsertenhanced green fluorescent protein
UseTagsGAL4 DNA Binding Domain and MycExpressionBacterial and YeastMutationPromoterCUP1Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
FRP1483
Plasmid#64511PurposeExpresses destabilized Ametrine in yeast.DepositorInsertAmetrine-ADH1-tail
UseTagsExpressionYeastMutationPromoterTDH3Available sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
FRP1432
Plasmid#64509PurposeExpresses destabilized Citrine in yeast.DepositorInsertCitrine-ADH1-tail
UseTagsExpressionYeastMutationPromoterTDH3Available sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only