We narrowed to 739 results for: Numb
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Plasmid#31915DepositorInsertSlow-FT
UseTagsExpressionMammalianMutationM18V/E30V/K69R/L84W/A179V compared to mCherry (bu…PromoterPTight Tet-responsive PminCMVdAvailable sinceDec. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTRE-Medium-FT
Plasmid#31914DepositorInsertMedium-FT
UseTagsExpressionMammalianMutationM18L/N23D/T43S/K69R/L84W/M152I/Q194L/L205M/Y221C/…PromoterPTight Tet-responsive PminCMVdAvailable sinceSept. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTRE-Fast-FT
Plasmid#31913DepositorInsertFast-FT
UseTagsExpressionMammalianMutationM18V/E34K/K69R/L84W/S149T/A224S compared to mCher…PromoterPTight Tet-responsive PminCMVdAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFast-FT-N1
Plasmid#31910DepositorInsertFast-FT
UseTagsExpressionMammalianMutationM18V/E34K/K69R/L84W/S149T/A224S compared to mCher…PromoterCMVAvailable sinceSept. 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pPAmCherry2-C1
Plasmid#31932DepositorInsertPAmCherry2
UseTagsExpressionMammalianMutationM18L/K69N/L84F/A147T/S148L/I165L/Q167A/L169T/A179…PromoterCMVAvailable sinceSept. 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SnoopCatcher
Plasmid#72322PurposeBacterial expression of SnoopCatcher, an engineered protein forming a spontaneous covalent bond with its cognate peptide partner SnoopTag.DepositorInsertpET28a-SnoopCatcher
UseTagsHis6 tagExpressionBacterialMutationchanged G842T and D848G of S. pneumoniae adhesin …PromoterT7Available sinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAC-94N
Plasmid#53281PurposeContains crtI, crtY, and crtI genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and produces beta-carotene in E. coli when complemented with a gene encoding geranylgeranyl diphosphate synthaseDepositorInsertcrtY, crtI, crtB
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMedium-FT-N1
Plasmid#31911DepositorInsertMedium-FT
UseTagsExpressionMammalianMutationM18L/N23D/T43S/K69R/L84W/M152I/Q194L/L205M/Y221C/…PromoterCMVAvailable sinceSept. 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHsp70-Cas9
Plasmid#45945PurposeThe codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.DepositorInsertCodon optimized Cas9
UseCRISPRTags3x FLAGExpressionMutationSilent change from C to A at bp 834 of insertPromoterAvailable sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAC-PHYTipi
Plasmid#53283PurposeContains crtE, crtB, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces phytoene in E. coliDepositorInsertcrtE, idi, crtY, crtI, crtB
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTfR-PAmKate
Plasmid#32692DepositorInsertPAmKate
UseTagstransferrin receptor (TfR)ExpressionMammalianMutationF84W/S148N/S165G compared to mKate (but numbering…PromoterCMVAvailable sinceOct. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pPAmCherry-b-actin
Plasmid#31949DepositorInsertPAmCherry1
UseTagsb-actinExpressionMammalianMutationE26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…PromoterCMVAvailable sinceSept. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pACT5FVMF(f)
Plasmid#110549PurposeControl plasmid for titering FV by qPCR to determine the genome-containing particle number. This qPCR protocol is described in Hendrie et al., 2008DepositorInsertEGFP, FV LTRs
UseQpcr control for titering foamy virusTagsExpressionMutationPromoterpromoter: MLV LTRAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKERep
Plasmid#211826PurposeMedium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.DepositorInserteGFP
UseTagsExpressionBacterialMutationPromoterT5Available sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKESa
Plasmid#211828PurposeLow copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.DepositorInserteGFP
UseTagsExpressionBacterialMutationPromoterT5Available sinceFeb. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAC-ZEAX
Plasmid#53274PurposeContains crtE, crtB, crtI, crtY and crtZ genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces zeaxanthin in E. coliDepositorInsertcrtE, crtY, crtI, crtB, crtZ
UseLow copy number bacterial cloning vectorTagsExpressionMutationDeleted idi (ORF6) and crtX genesPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-PIK3C2B
Plasmid#161989PurposeMammalian expression vector containing PIK3C2B tagged with GFPDepositorInsertPIK3C2B (PIK3C2B Human)
UseTagsGFPExpressionMammalianMutationsiRNA resistance to siRNA#2 with 4 silent mutatio…PromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB-PAmCherry1
Plasmid#31931DepositorInsertPAmCherry1
UseTagsHisExpressionBacterialMutationE26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…PromoteraraBAvailable sinceSept. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTfR-PAmCherry1
Plasmid#31948DepositorInsertPAmCherry1
UseTagstransferrin receptor (TfR)ExpressionMammalianMutationE26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…PromoterCMVAvailable sinceSept. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB-Fast-FT
Plasmid#31916DepositorInsertFast-FT
UseTagsHisExpressionBacterialMutationM18V/E34K/K69R/L84W/S149T/A224S compared to mCher…PromoteraraBAvailable sinceSept. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAC-GGPPipi
Plasmid#53280PurposeContains crtE and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces geranylgeranyl diphosphate in E. coliDepositorInsertcrtE, idi
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKHR5
Plasmid#74593Purposean alpha-crystallin::Venus (green lens) reporter cassette flanked by FRT sites and with a single loxP site at the 3’ end was inserted in the middle of pKHR4 mcs. Genbank accession number is KU144823.DepositorInsertFRT-alpha-crystallin::Venus-FRT-loxP reporter cassette
UseCre/Lox; Zebrafish expressionTagsExpressionMutationPromoteralpha-crystallinAvailable sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pICH-roGFP2-ORP1
Plasmid#191677PurposeTi plasmid pICH86988 harbouring roGFP2-ORP1 for peroxide detection in plant cells.DepositorInsertredox(ro)GFP2-ORP1
UseBinary agrobacterium ti plasmidTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pICH-chl-roGFP2-synORP1
Plasmid#191701PurposeTi plasmid pICH86988 harbouring chl-roGFP2-synORP1 for H2O2 detection in the plastids of plant cells.DepositorInsertchl-roGFP2-synORP1
UseBinary agrobacterium ti plasmidTagsExpressionPlantMutationPromoterCaMV 35S promoterAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pICH-nuc-roGFP2-synORP1
Plasmid#191759PurposeTi plasmid pICH86988 harbouring nuc-roGFP2-synORP1 for H2O2 detection in the nuclei of plant cells.DepositorInsertnuc-roGFP2-synORP1
UseBinary agrobacterium ti plasmidTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAC-crRNA-Km
Plasmid#158712PurposePlasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNADepositorInsertAcGFP (for crRNA cloning)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterTetOAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAC-ZETAipi
Plasmid#53284PurposeContains crtE, crtB, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and pds (crtP) gene of Synechococcus PCC7942, and thereby produces zeta-carotene in E. coliDepositorInsertpds
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterlacAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJUMP18-Uac
Plasmid#126959PurposeUniversal Acceptor Plasmid. The fusion sites of the basic part generated are introduced during domestication. sfGFP reporter gene. High copy number oriV #8.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pICH-nuc-roGFP2-ORP1
Plasmid#191758PurposeTi plasmid pICH86988 harbouring nuc-roGFP2-ORP1 for peroxide detection in the nuclei of plant cells.DepositorInsertnuc-roGFP2-ORP1
UseBinary agrobacterium ti plasmidTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB-Medium-FT
Plasmid#31917DepositorInsertMedium-FT
UseTagsHisExpressionBacterialMutationM18L/N23D/T43S/K69R/L84W/M152I/Q194L/L205M/Y221C/…PromoteraraBAvailable sinceSept. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB-Slow-FT
Plasmid#31918DepositorInsertSlow-FT
UseTagsHisExpressionBacterialMutationM18V/E30V/K69R/L84W/A179V compared to mCherry (bu…PromoteraraBAvailable sinceSept. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAC-ZETA
Plasmid#53316PurposeContains Erwinia herbicola Eho10 genes crtE and crtB, together with the pds (crtP) gene of Synechococcus PCC7942 fused to the lacZ gene at the N terminus. Produces zeta-carotene in E. coli.DepositorInsertpds
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterlazZAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOGG010
Plasmid#113984PurposeVector construction module. RK2 (IncP) low-copy number origin of replication for construction of Golden Gate cloning vectorsDepositorInsertRK2 (IncP)
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC-NEUR
Plasmid#53271PurposeContains the Erwinia herbicola (Pantoea agglomerans) Eho10 genes crtE and crtB, and the Rhodobacter capsulatus crtI gene and thereby produces neurosporene in Escherichia coliDepositorInsertcrtI
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1149 - intron 3 smu-2 PATCs
Plasmid#161516PurposePATC-rich intron number three from smu-2 to stimulate germline expression in C. elegansDepositorInsertIntron 3 from smu-2
UseTagsExpressionWormMutationPromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pICH-chl-roGFP2-ORP1
Plasmid#191699PurposeTi plasmid pICH86988 harbouring chl-roGFP2-ORP1 for peroxide detection in the plastids of plant cells.DepositorInsertchl-roGFP2-ORP1
UseBinary agrobacterium ti plasmidTagsExpressionPlantMutationPromoterCaMV 35S promoterAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAC-PHYT
Plasmid#53300PurposeContains Erwinia herbicola Eho10 (Pantoea agglomerans) crtE and crtB genes, and thereby produces phytoene in E. coliDepositorInsertcrtE, crtB
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
RAB14 3'UTR WT
Plasmid#61489Purposeluciferase reporter containing RAB14 3' UTRDepositorInsertRAB14 3' UTR (RAB14 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationcontains position 837-1637 (numbering relative to…PromoterPGKAvailable sinceJan. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJUMP27ts[mCherry]-1A(lacZ)
Plasmid#126980PurposeLevel 1 vector. With lacZ as alternative cloning reporter. Thermosensitive origin pSC101 (low copy number). Constitutive mCherry in downstream secondary site.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationRepA A56V thermosensitive mutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCA0.002
Plasmid#119560PurposeLevel 0 acceptor vector for promoters with lacZ compatible with blue/white screening, low copy number, pSC101 ori.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only