Addgene

We narrowed to 1,051 results for: SEF;

Showing: 481 - 520 of 1051 results

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pCASP-HilA empty vector

Plasmid

#153333

Purpose

Triggers HilA overexpression from Salmonella upon induction with arabinose without any synthetic protein expressed

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Type

Empty backbone

Use

Salmonella expression

Tags

Expression

Mutation

Promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pIIS18-BsmBI

Plasmid

#128648

Purpose

Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

Depositor

Tohru Suzuki

Article

Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1-mSNX27 RNAi-resistant

Plasmid

#163620

Purpose

mouse SNX27 cDNA with point mutation to resist siRNA against SNX27 C-terminally tagged with GFP

Depositor

Josef Kittler

Article

Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

Insert

mouse SNX27 RNAi-resistant (Snx27 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

RNAi resistance

Promoter

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
SRP19 in pLX311

Plasmid

#233292

Purpose

Expression of WT SRP19

Depositor

Sefi Rosenbluh

Insert

SRP19 (SRP19 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

E1Fa

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pLEX302_circSMAD2_OE

Plasmid

#215238

Purpose

Overexpression of pLEX302_circSMAD2(2,7)_OE expression

Depositor

Sefi Rosenbluh

Article

Liu et al Nat Cell Biol. 2024 Aug;26(8):1359-1372.

Insert

circSMAD2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
shRNA_LacZ

Plasmid

#215217

Purpose

Supression of shLacZ expression (Negative control)

Depositor

Sefi Rosenbluh

Article

Liu et al Nat Cell Biol. 2024 Aug;26(8):1359-1372.

Insert

LacZ shRNA

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pIIS18-BufAI-2

Plasmid

#128651

Purpose

Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

Depositor

Tohru Suzuki

Article

Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pIIS18-BufAI-1

Plasmid

#128650

Purpose

Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

Depositor

Tohru Suzuki

Article

Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-Trak2

Plasmid

#127622

Purpose

N-terminal GFP-tagged mouse Trak2

Depositor

Josef Kittler

Article

Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

Insert

Trak2 (Trak2 Mouse)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA Flag Yap1

Plasmid

#18881

Depositor

Yosef Shaul

Article

Levy et al Mol Cell. 2008 Feb 15. 29(3):350-61.

Insert

Yap1 (YAP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 14, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-Trak1

Plasmid

#127621

Purpose

N-terminal GFP-tagged mouse Trak1

Depositor

Josef Kittler

Article

Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

Insert

Trak1 (Trak1 Mouse)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-GLT1a

Plasmid

#162515

Purpose

Rat GLT-1a cDNA N-terminally tagged with EGFP

Depositor

Josef Kittler

Article

Al Awabdh et al Glia. 2016 Jul;64(7):1252-64. doi: 10.10

Insert

GLT1a (Slc1a2 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3 NQO1

Plasmid

#61730

Purpose

Expresses WT human NQO1

Depositor

Yosef Shaul

Article

Adamovich et al Mol Cell Biol. 2013 Jul;33(13):2603-13.

Insert

NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)

Use

Tags

None

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-GLT1b

Plasmid

#162516

Purpose

Rat GLT-1b cDNA N-terminally tagged with EGFP

Depositor

Josef Kittler

Article

Al Awabdh et al Glia. 2016 Jul;64(7):1252-64. doi: 10.10

Insert

GLT1b (Slc1a2 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-gRNA-puro

Plasmid

#180426

Purpose

Expresses puromycin resistance gene and scrambled gRNA for stable integration in mammalian cells; useful as control and template for new gRNAs by mutagenesis.

Depositor

Moshe Szyf

Article

Sapozhnikov et al Nat Commun. 2021 Sep 29;12(1):5711. doi:

Insert

gRNAscr

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
POT2-RFP

Plasmid

#65318

Purpose

receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCASP-SptP120-K55-HilA

Plasmid

#153329

Purpose

Triggers secretion of SptP120 fused to the K55 DARPin from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-K55 DARPin

Use

Salmonella expression

Tags

Expression

Mutation

Promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
POT4-RFP

Plasmid

#65320

Purpose

receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCASP-SptP120-3G124-HilA

Plasmid

#153323

Purpose

Triggers secretion of SptP120 fused to the 3G124nc anti-GFP DARPin from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-3G124 DARPin

Use

Salmonella expression

Tags

Expression

Mutation

Promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-GLT1a-V5

Plasmid

#162517

Purpose

Rat GLT-1a cDNA N-terminally tagged with EGFP with a v5 tag in the extracellular loop

Depositor

Josef Kittler

Article

Al Awabdh et al Glia. 2016 Jul;64(7):1252-64. doi: 10.10

Insert

GLT1a (Slc1a2 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-GLT1b-V5

Plasmid

#162518

Purpose

Rat GLT-1a cDNA N-terminally tagged with EGFP with a v5 tag in the extracellular loop

Depositor

Josef Kittler

Article

Al Awabdh et al Glia. 2016 Jul;64(7):1252-64. doi: 10.10

Insert

GLT1b (Slc1a2 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJOE5751.1

Plasmid

#135083

Purpose

pBR322-based L-rhamnose-inducible vector.

Depositor

Josef Altenbuchner

Article

Hoffmann et al FEBS J. 2012 Oct;279(20):3911-24. doi: 1

Insert

P-rhaP-BAD-His tag-eGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCASP-SptP120-TD-HilA

Plasmid

#153330

Purpose

Triggers secretion of SptP120 fused to the HA4-7c12 Tandem Monobody from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-HA4-7c12 Tandem Monobody

Use

Salmonella expression

Tags

Expression

Mutation

Promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCASP-SptP167-3G124-HilA

Plasmid

#153322

Purpose

Triggers secretion of SptP167 fused to the 3G124nc anti-GFP DARPin from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP167-3G124 DARPin

Use

Salmonella expression

Tags

Expression

Mutation

Promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCASP-SptP120-3m160-HilA

Plasmid

#153325

Purpose

Triggers secretion of SptP120 fused to the 3m160 anti-mCherry DARPin from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-3m160 DARPin

Use

Salmonella expression

Tags

Expression

Mutation

Promoter

Available since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
POT10-RFP

Plasmid

#65326

Purpose

receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
POT8-RFP

Plasmid

#65324

Purpose

receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCASP-SptP120-E3_5-HilA

Plasmid

#153326

Purpose

Triggers secretion of SptP120 fused to the E3_5 DARPin from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-E3_5 DARPin

Use

Salmonella expression

Tags

Expression

Mutation

Promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
POT7-RFP

Plasmid

#65323

Purpose

receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
POT6-RFP

Plasmid

#65322

Purpose

receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCASP-SptP120-K27-HilA

Plasmid

#153328

Purpose

Triggers secretion of SptP120 fused to the K27 DARPin from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-K27 DARPin

Use

Salmonella expression

Tags

Expression

Mutation

Promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
POT11-RFP

Plasmid

#65327

Purpose

receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
POT5-RFP

Plasmid

#65321

Purpose

receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
POT3-RFP

Plasmid

#65319

Purpose

receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
POT9-RFP

Plasmid

#65325

Purpose

receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCASP-SptP120-NS1-HilA

Plasmid

#153327

Purpose

Triggers secretion of SptP120 fused to the NS1 monobody from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-NS1 Monobody

Use

Salmonella expression

Tags

Expression

Mutation

Promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJOE6089.4

Plasmid

#135081

Purpose

pBR322-based L-rhamnose-inducible vector.

Depositor

Josef Altenbuchner

Article

Hoffmann et al FEBS J. 2012 Oct;279(20):3911-24. doi: 1

Insert

P-rhaP-BAD-eGFP-Strep-tag II

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
SRP19 (siRNA resistant) in pLX311

Plasmid

#233291

Purpose

Expression of WT SRP19 (siRNA resistant)

Depositor

Sefi Rosenbluh

Insert

SRP19 (SRP19 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Expresses WT SRP19 that is resistant to siRNA

Promoter

E1Fa

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28Man

Plasmid

#203399

Purpose

exo-beta mannosidase useful in enzymatic biotransformations of glycans and in coupled assays of various carbohydrate processing enzymes

Depositor

David Vocadlo

Article

Deng et al Bioorg Med Chem. 2013 Aug 15;21(16):4839

Insert

man2A

Use

Tags

H6

Expression

Bacterial

Mutation

None

Promoter

T7

Available since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1-mSNX27-H112A RNAi-resistant

Plasmid

#163621

Purpose

mouse SNX27 cDNA with point mutation to resist siRNA against SNX27 and with a point mutation H112A C-terminally tagged with GFP

Depositor

Josef Kittler

Article

Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

Insert

mouse SNX27-H112A RNAi-resistant (Snx27 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

H112A and RNAi resistance

Promoter

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 481 - 520 of 1051 results

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