We narrowed to 10,893 results for: ENA;

1. pQE60 P126R wgMDH

   Plasmid

   #204261

   Purpose

   Bacterial expression of P126R wgMDH with IPTG induction - Active site loop mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   P126R

   Promoter

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pQE60 G127A wgMDH

   Plasmid

   #204263

   Purpose

   Bacterial expression of G127A wgMDH with IPTG induction - Active site loop mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   G127A

   Promoter

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pQE60 M66L wgMDH

   Plasmid

   #204247

   Purpose

   Bacterial expression of M66L wgMDH with IPTG induction - Subunit interface mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   M66L

   Promoter

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pQE60 G127P wgMDH

   Plasmid

   #204265

   Purpose

   Bacterial expression of G127P wgMDH with IPTG induction - Active site loop mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   G127P

   Promoter

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pQE60 G121F wgMDH

   Plasmid

   #204253

   Purpose

   Bacterial expression of G121F wgMDH with IPTG induction - Active site loop mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   G121F

   Promoter

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pQE60 M91Q wgMDH

   Plasmid

   #204250

   Purpose

   Bacterial expression of M91Q wgMDH with IPTG induction - Subunit interface mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   M91Q

   Promoter

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pQE60 D87L wgMDH

   Plasmid

   #204248

   Purpose

   Bacterial expression of D87L wgMDH with IPTG induction - Subunit interface mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   D87L

   Promoter

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pQE60 P126G wgMDH

   Plasmid

   #204260

   Purpose

   Bacterial expression of P126G wgMDH with IPTG induction - Active site loop mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   P126G

   Promoter

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pQE60 P123G wgMDH

   Plasmid

   #204256

   Purpose

   Bacterial expression of P123G wgMDH with IPTG induction - Active site loop mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   P123G

   Promoter

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pQE60 P123F wgMDH

    Plasmid

    #204255

    Purpose

    Bacterial expression of P123F wgMDH with IPTG induction - Active site loop mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    P123F

    Promoter

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPD1cR

    Plasmid

    #86970

    Purpose

    Expresses PCNA1 variant (G108C) fused phosphite dehydrogenase and putidaredoxin reductase in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038

    Insert

    PCNA1 variant (G108C) fused to phosphite dehydrogenase and putidaredoxin reductase
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    E175A/A176R in PTDH, G108C in PCNA1

    Promoter

    T7 promoter

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPD2cX

    Plasmid

    #86968

    Purpose

    Expresses PCNA2 variant (L171C) fused phosphite dehydrogenase and putidaredoxin in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038

    Insert

    PCNA2 variant (L171C) fused to phosphite dehydrogenase and putidaredoxin
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    E175A/A176R in PTDH, C73S/C85S in PdX, L171C in P…

    Promoter

    T7 promoter

    Available Since

    March 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCOLA-Gent-EM7-Erv1p-DsbC

    Plasmid

    #202485

    Purpose

    Expresses sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) constitutively

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) (ERV1 Escherichia coli, Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    EM7

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CRISPR-StAR4BN (Lenti)

    Plasmid

    #222692

    Purpose

    CRISPR-screening

    Depositor

    Ulrich Elling

    Article

    Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    PGK without BsmBI cutsite

    Promoter

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CAG-mCherry

    Plasmid

    #108685

    Purpose

    Strong CAG promoter with mCherry expression for transfection efficacy screening

    Depositor

    Jordan Green

    Article

    Mishra et al Regen Eng Transl Med. 2019 Sep;6(3):273-

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CRISPR-StAR4GN (Lenti)

    Plasmid

    #222693

    Purpose

    CRISPR-screening

    Depositor

    Ulrich Elling

    Article

    Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    PGK without BsmBI cutsite

    Promoter

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CRISPR-StAR4B (Lenti)

    Plasmid

    #222691

    Purpose

    CRISPR-screening

    Depositor

    Ulrich Elling

    Article

    Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    PGK without BsmBI cutsite

    Promoter

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CRISPR-StAR4 (Retro)

    Plasmid

    #222690

    Purpose

    CRISPR-screening

    Depositor

    Ulrich Elling

    Article

    Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    PGK without BbsI cutsite

    Promoter

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCOLA-Gent-EM7-Erv1p-PDI

    Plasmid

    #202486

    Purpose

    Expresses sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) constitutively

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) (ERV1 Human, Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    EM7

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a hMDH1v3 (+TEV)

    Plasmid

    #204229

    Purpose

    Bacterial expression of human cytosolic malate dehydrogenase isoform 1 transcript variant 3 (hMDH1v3); Use in CURES (integration of research into undergraduate teaching labs).

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Insert

    hMDH1v3 (MDH1 Human)
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Inserted a G after ATG to maintain kozak site

    Promoter

    Lac

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge