We narrowed to 386 results for: SaCas9

1. pJEP322-pAAV-FullU6TO-SaCa9sgRNAi(emx1-sg1)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA

   Plasmid

   #113699

   Purpose

   Dox-inducible U6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1. Followed by an EFS driven GFP-KASH in a separate reading frame.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Inserts

   SaCas9 gRNA Cassete
   GFP
   

   Use

   AAV and CRISPR

   Tags

   KASH

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSP2292

   Plasmid

   #70707

   Purpose

   Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Mutation

   E782K, N968K and R1015H in SaCas9

   Promoter

   T7

   Available Since

   Dec. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSP2253

   Plasmid

   #70704

   Purpose

   Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Mutation

   E782K, N968K and R1015H in SaCas9

   Promoter

   T7

   Available Since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-SaPE2

   Plasmid

   #174817

   Purpose

   Mammalian expression of SaCas9 prime editor 2

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   SaPE2
   

   Tags

   SV40 bpNLS

   Expression

   Mammalian

   Mutation

   Detailed in manuscript

   Promoter

   CMV

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV- GfaABC1D::Cas9-HA-sgRNA-Non-Targeting

   Plasmid

   #241026

   Purpose

   GfaABC1D promoter driven HA-tagged SaCas9 together with U6 driven expression of a non-targeting sgRNA; used as control for SaCas9 based knock-outs in murine astrocytes

   Depositor

   Mark Verheijen

   Article

   Badia-Soteras et al Biol Psychiatry. 2023 Aug 1;94(3):226-23

   Insert

   U6 driven empty sgRNA
   

   Use

   AAV and CRISPR

   Tags

   SaCas9 + 3X HA-Tag

   Expression

   Mammalian

   Promoter

   GfaABC1D

   Available Since

   Aug. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-hSyn-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA

   Plasmid

   #177360

   Purpose

   AAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expression from Synapsin promoter

   Depositor

   Naoki Yamamoto

   Article

   Yamamoto et al Research Square [Preprint] 2021. doi: 10

   Inserts

   tdTomato
   Chimeric guide for SaCas9
   

   Use

   AAV

   Tags

   P2A and a tetracycline-controlled transactivator …

   Expression

   Mammalian

   Promoter

   Synapsine promoter and U6 promoter

   Available Since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-CMV-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA

   Plasmid

   #177365

   Purpose

   AAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expression

   Depositor

   Naoki Yamamoto

   Article

   Yamamoto et al Research Square [Preprint] 2021. doi: 10

   Inserts

   tdTomato
   Chimeric guide for SaCas9
   

   Use

   AAV

   Tags

   P2A and a tetracycline-controlled transactivator …

   Expression

   Mammalian

   Promoter

   CMV and U6 promoter

   Available Since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFH14

   Plasmid

   #125799

   Purpose

   Level0 SaCas9, Arabidopsis codon optimized

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   SaCas9, Arabidopsis codon optimized
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-SaPE2-P2A-BSD

   Plasmid

   #174818

   Purpose

   Mammalian expression of SaCas9 prime editor 2 with P2A-BSD marker

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   SaPE2-P2A-BSD
   

   Tags

   SV40 bpNLS

   Expression

   Mammalian

   Mutation

   Detailed in manuscript

   Promoter

   CMV

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFH58

    Plasmid

    #128184

    Purpose

    Level1 ZmUBIp::SaCas9 (Arabidopsis codon optimized):NOSt

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    ZmUBIp::SaCas9 (Arabidopsis codon optimized):NOSt
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFH102

    Plasmid

    #128207

    Purpose

    sgRNA backbone for SaCas9, combine with AtU6-26 promoter module (pFH35)

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    sgRNA backbone for SaCas9, combine with AtU6-26 promoter module (pFH35)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEPOR0SP0020

    Plasmid

    #117531

    Purpose

    Level0 Golden Gate part: CDS, SaCas9

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    SaCas9 (with stop codon)
    

    Use

    CRISPR

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLdSaCN

    Plasmid

    #123261

    Purpose

    Expresses Staphylococcus aureus Cas9 (SaCas9) and its gRNA in Leishmania

    Depositor

    Greg Matlashewski

    Article

    Zhang et al mSphere. 2019 Aug 21;4(4). pii: 4/4/e004

    Insert

    gRNA and SaCas9
    

    Use

    CRISPR; Leishmania

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFH88

    Plasmid

    #128201

    Purpose

    sgRNA backbone for SaCas9, combine with TaU3 promoter module (pFH33)

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    sgRNA backbone for SaCas9, combine with TaU3 promoter module (pFH33)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEPOR1CB0058

    Plasmid

    #117539

    Purpose

    Level1 Golden Gate Cassette: sgRNA cassette for SaCas9, targeting NbPDS gene in N. benthamiana

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    sgRNA (SaCas9)
    

    Expression

    Plant

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEPOR0SP0021

    Plasmid

    #117532

    Purpose

    Level0 Golden Gate part: CDS, SaCas9 (no stop codon)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    SaCas9 (no stop codon)
    

    Use

    CRISPR

    Available Since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAID6

    Plasmid

    #136989

    Purpose

    Yeast integration plasmid for expression of dLbCpf1-VP, dSpCas9-RD1152, SaCas9, and Csy4

    Depositor

    Huimin Zhao

    Article

    Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

    Inserts

    dLbCpf1-VP
    dSpCas9-RD1152
    SaCas9
    Csy4
    

    Expression

    Yeast

    Promoter

    ENO2, TDH3, TEF1, and TPI1

    Available Since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-FLEX-EGFP-KASH

    Plasmid

    #154373

    Purpose

    Vector for Cre-dependent expression of EGFP tagged KASH protein domain

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    EGFP-KASH
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Available Since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-FLEXfrt-EGFP-KASH

    Plasmid

    #154374

    Purpose

    Vector for Flp-dependent expression of EGFP tagged KASH protein domain

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    EGFP-KASH
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEPOR1CB0015

    Plasmid

    #117551

    Purpose

    Level 1 Golden Gate Cassette: SaCas9 expression cassette for dicotyledonous plants

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    2x35S+5'UTR OMEGA (pICH51288) + SaCas9 (no stop codon) (pEPOR0SP0012) + YFP (pICSL50005) + Nos (pICH41421)
    

    Expression

    Plant

    Promoter

    2x35S+5'UTR OMEGA (pICH51288)

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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