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pX458-Ef1a-Cas9-H2B-mCherry

Plasmid

#159655

Purpose

Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B mCherry nuclear reporter.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-GFP

Plasmid

#159654

Purpose

Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B GFP nuclear reporter.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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Biomolecular FeedBack (FB)

Plasmid

#109391

Purpose

This construct encodes for two units. The first contains a constitutively expressed dCas9 protein. The second contains an sgRNA cassette under the control of the burden-responsive htpG1 promoter.

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Insert

pJ23100mut-dCas9-T- phtpG1-sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-Voltron

Plasmid

#119033

Purpose

Express Voltron in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

Insert

Voltron

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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myristoylated ct-GRK2

Plasmid

#178734

Purpose

N-myristoylation signaling peptide of Src and the C-terminal fragment of G-protein coupled receptor kinase 2 (ct-GRK2)

Depositor

Ege Kavalali

Article

Alten et al Cell Rep. 2022 Jan 18;38(3):110255. doi:

Insert

GRK2 (GRK2 Bovine)

Use

Lentiviral

Tags

Src 1-15

Expression

Mutation

only amino acids 548-671

Promoter

Ubiquitin

Available since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-2)

Plasmid

#159660

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Ef1a

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-3)

Plasmid

#159661

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Ef1a

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-1)

Plasmid

#159659

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Ef1a

Available since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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MAC_C_FGFR2

Plasmid

#187751

Purpose

MAC-tagged gene expression of human FGFR2

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

FGFR2 (FGFR2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_C_ERBB2

Plasmid

#187776

Purpose

MAC-tagged gene expression of human ERBB2

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

ERBB2 (ERBB2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-3)

Plasmid

#159658

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Ef1a

Available since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-2)

Plasmid

#159657

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Ef1a

Available since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-WDR61

Plasmid

#231729

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Martinez et al Molecular Cell. 01 Mar 2025. doi: 10.101

Insert

WDR61 (SKIC8 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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MAC_C_CSF1R

Plasmid

#187767

Purpose

MAC-tagged gene expression of human CSF1R

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

CSF1R (CSF1R Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_C_ERBB3

Plasmid

#187794

Purpose

MAC-tagged gene expression of human ERBB3

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

ERBB3 (ERBB3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pDON207 NSP3 K977Q

Plasmid

#180057

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 NSP3 K977Q (ORF1ab SARS-CoV-2)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

NSP3 K977Q

Promoter

Available since

Jan. 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDON207 NSP3 A890D

Plasmid

#180069

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 NSP3 A890D (ORF1ab SARS-CoV-2)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

NSP3 A890D

Promoter

Available since

Jan. 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDON221 ORF3a Q57H

Plasmid

#180072

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 ORF3a Q57H (ORF3a SARS-CoV-2)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

ORF3a Q57H

Promoter

Available since

Jan. 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDON223 N S235F

Plasmid

#180071

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 N S235F (N SARS-CoV-2)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

N S235F

Promoter

Available since

Jan. 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDON223 NSP5 K90R

Plasmid

#180073

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 NSP5 K90R (ORF1ab SARS-CoV-2)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

NSP5 K90R

Promoter

Available since

Jan. 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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