We narrowed to 10,449 results for: T7

1. pCMV-T7-hAcrVA3.1-NLS(sv40) (RTW2624)

   Plasmid

   #115139

   Purpose

   CMV-T7 promoter expression plasmid for human codon optimized AcrVA3.1 anti-CRISPR protein with C-terminal NLS

   Depositor

   Benjamin Kleinstiver, Keith Joung, Joseph Bondy-Denomy

   Article

   Marino et al Science. 2018 Sep 6. pii: science.aau517

   Insert

   human codon optimized AcrVA3.1 anti-CRISPR protein
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-T7-SpG-P2A-mScarlet (MNW006)

   Plasmid

   #174136

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized SpG with BPNLS-3xFLAG-P2A-mScarlet
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-mScarlet

   Expression

   Mammalian

   Mutation

   SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

   Promoter

   CMV and T7

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-T7-hAcrVA3-NLS(sv40) (BPK5077)

   Plasmid

   #115140

   Purpose

   CMV-T7 promoter expression plasmid for human codon optimized AcrVA3 anti-CRISPR protein with C-terminal NLS

   Depositor

   Benjamin Kleinstiver, Keith Joung, Joseph Bondy-Denomy

   Article

   Marino et al Science. 2018 Sep 6. pii: science.aau517

   Insert

   human codon optimized AcrVA3 anti-CRISPR protein
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-T7-hAcrVA2-NLS(sv40) (AAS2283)

   Plasmid

   #115138

   Purpose

   CMV-T7 promoter expression plasmid for human codon optimized AcrVA2 anti-CRISPR protein with C-terminal NLS

   Depositor

   Benjamin Kleinstiver, Keith Joung, Joseph Bondy-Denomy

   Article

   Marino et al Science. 2018 Sep 6. pii: science.aau517

   Insert

   human codon optimized AcrVA2 anti-CRISPR protein
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEX-4T-1-p19-T7-EGFPFL

   Plasmid

   #46310

   Depositor

   Judy Lieberman

   Article

   Huang et al Nat Biotechnol. 2013 Apr;31(4):350-6. do

   Inserts

   p19
   EGFPFL DNA-1
   linker
   EGFPFL DNA-2
   

   Use

   Pro-sirna

   Tags

   6XHis and GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pPGK-T7/2-CD44v8-10 (-cyt)

   Plasmid

   #137822

   Purpose

   expression of CD44 proteins in mammalian cells

   Depositor

   Ursula Gunthert

   Article

   Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

   Insert

   CD44v8-10 (-cyt) (CD44 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   without cytoplasmic region

   Promoter

   PGK

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-T7-AcrIIA17_Sga-NLS(SV40) (KAC92)

   Plasmid

   #134334

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIA17_Sga with C-terminal NLS (SV40)

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized AcrIIA17_Sga
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-T7-AcrIIA18_Sma-NLS(SV40) (KAC95)

   Plasmid

   #134335

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIA18_Sma with C-terminal NLS (SV40)

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized AcrIIA18_Sma
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-T7-AcrIIA19_Ssim-NLS(SV40) (KAC97)

   Plasmid

   #134336

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIA19_Ssim with C-terminal NLS (SV40)

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized AcrIIA19_Ssim
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-T7-AcrIIA18_Sga-NLS(SV40) (KAC214)

    Plasmid

    #134338

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA18_Sga with C-terminal NLS (SV40)

    Depositor

    Benjamin Kleinstiver, Joseph Bondy-Denomy

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized AcrIIA18_Sga
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-T7-AcrIIA19_Spse-NLS(SV40) (KAC215)

    Plasmid

    #134339

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA19_Spse with C-terminal NLS (SV40)

    Depositor

    Benjamin Kleinstiver, Joseph Bondy-Denomy

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized AcrIIA19_Spse
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEX-4T-1-p19-T7-GagB500

    Plasmid

    #46314

    Depositor

    Judy Lieberman

    Article

    Huang et al Nat Biotechnol. 2013 Apr;31(4):350-6. do

    Inserts

    p19
    GagB500 DNA-1
    linker
    GagB500 DNA-2
    

    Use

    Pro-sirna

    Tags

    6XHis and GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

13. psbA2-Ptrc-T7-g10-PHLS (e)

    Plasmid

    #52310

    Purpose

    Replaces the psbA2 gene in Synechocystis with the Ptrc promoter-T7-g10-PHLS gene

    Depositor

    Anastasios Melis

    Article

    Formighieri et al Planta. 2014 Aug;240(2):309-24. doi: 10.

    Inserts

    beta-phellandrene synthase
    Chloramphenicol resistance
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptrc-T7

    Available since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCBh-T7-SpG-P2A-mTagBFP2(RAS3583)

    Plasmid

    #223110

    Purpose

    pCBh Human expression plasmid for SpG with with C-term BPNLS-P2A-mTagBFP2

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpG-P2A-mTagBFP2
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-mTagBFP2

    Expression

    Mammalian

    Mutation

    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCBh-T7-SpCas9-P2A-mTagBFP2(RAS3575)

    Plasmid

    #223111

    Purpose

    pCBh Human expression plasmid for SpCas9 with with C-term BPNLS-P2A-mTagBFP2

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9-P2A-mTagBFP2
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. T7-RBS-6xHis-SUMO-SynCas.v10 bacteria

    Plasmid

    #208594

    Purpose

    Production and purification of catalytically active SynCas.v10

    Depositor

    Omar Akbari

    Article

    Brogan et al bioRxiv 2024.02.23.581838; doi: https://

    Insert

    SynCas.v10
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. T7-RBS-6xHis-SUMO-dSynCas.v10 bacteria

    Plasmid

    #208595

    Purpose

    Production and purification of catalytically inactive SynCas.v10

    Depositor

    Omar Akbari

    Article

    Brogan et al bioRxiv 2024.02.23.581838; doi: https://

    Insert

    dSynCas.v10
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. T7-RBS-6xHis-SUMO-SynCas.v12 bacteria

    Plasmid

    #208596

    Purpose

    Production and purification of catalytically active SynCas.v12

    Depositor

    Omar Akbari

    Article

    Brogan et al bioRxiv 2024.02.23.581838; doi: https://

    Insert

    SynCas.v12
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. T7-RBS-6xHis-SUMO-dSynCas.v12 bacteria

    Plasmid

    #208597

    Purpose

    Production and purification of catalytically inactive SynCas.v12

    Depositor

    Omar Akbari

    Article

    Brogan et al bioRxiv 2024.02.23.581838; doi: https://

    Insert

    dSynCas.v12
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. XZ073 (IVT) CMV-TO-T7-LIDARsensor-hybridUTR3

    Plasmid

    #233420

    Purpose

    IVT template for LIDAR reporter mRNA; mCherry as marker, EGFP as output

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    T7-LIDARsensor-hybridUTR3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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