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pInducer-FRA1

Plasmid

#192267

Purpose

Doxycycline-inducible expression of murine FRA1 (Fosl1)

Depositor

Günter Schneider

Article

Schneeweis et al Cell Mol Life Sci. 2022 Dec 19;80(1):12.

Insert

Fosl1 (Fosl1 Mouse)

Use

Lentiviral; Inducible expression

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pME18S-3HA-mULK1(K46N)

Plasmid

#22897

Depositor

Masaaki Muramatsu

Article

Yan et al Biochem Biophys Res Commun. 1998 May 8.

Insert

a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)

Use

Tags

3xHA

Expression

Mammalian

Mutation

changed Lysine 46 to Aspargine

Promoter

Available since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
AP1muA single guide RNA

Plasmid

#230030

Purpose

guide RNA for AP1muA tagging at the C-terminus in pX459

Depositor

Francesca Bottanelli

Article

Stockhammer et al J Cell Sci. 2024 Aug 15;137(16):jcs26195

Insert

AP1M1 guide (AP1M1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-EgC*

Plasmid

#20378

Depositor

Andrew Lee

Article

Hu et al Biochemistry. 2003 Dec 2. 42(47):13856-6

Insert

Eglin C wt*

Use

Tags

Expression

Bacterial

Mutation

F10W

Promoter

Available since

April 17, 2009

Availability

Academic Institutions and Nonprofits only

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SQ171fg/pCSacB

Plasmid

#69344

Purpose

SQ171 fast growing strain supported by pCSacB plasmid

Depositor

Michael Jewett

Article

Orelle et al Nature. 2015 Jul 29. doi: 10.1038/nature

Insert

pCSacB

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pRKVSV FRNK

Plasmid

#50534

Depositor

Kenneth Yamada

Article

Segarra et al FASEB J. 2005 Nov;19(13):1875-7.

Insert

protein tyrosine kinase, domain

Use

Tags

2X VSV

Expression

Mammalian

Mutation

truncated (NT2186-3268)

Promoter

CMV

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pRKVSV FAT

Plasmid

#50533

Depositor

Kenneth Yamada

Article

Segarra et al FASEB J. 2005 Nov;19(13):1875-7.

Insert

protein tyrosine kinase, domain

Use

Tags

2X VSV

Expression

Mammalian

Mutation

truncated (NT2630-3268)

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pCAGGS-N-TEV-Twin-Strep

Plasmid

#202526

Purpose

Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in mammalian cell

Depositor

Chiaki Murakami

Article

Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

Type

Empty backbone

Use

Tags

TEV cleavable site and Twin-strep-tag

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCI syn iGluSnFR

Plasmid

#106123

Purpose

Indicator for glutamate imaging (synapsin promoter)

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluSnFR

Use

Tags

Myc and PDGFR

Expression

Mammalian

Mutation

Promoter

synapsin

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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FGF10-luciferase

Plasmid

#24994

Depositor

Benoit Bruneau

Article

Agarwal et al Development. 2003 Feb . 130(3):623-33.

Insert

Fgf10 promoter (Fgf10 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2010

Availability

Academic Institutions and Nonprofits only
pCAGGS-N-TEV-HAT

Plasmid

#202598

Purpose

Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cell

Depositor

Chiaki Murakami

Article

Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

Type

Empty backbone

Use

Tags

TEV cleavable site (ENLYFQG) and poly-histidine a…

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pLEX_305-N-dTAG-FRA1

Plasmid

#188742

Purpose

expresses murine Fosl1 (Fra1) with N-terminal tag FKBP F36V (dTAG) for the dTAG system

Depositor

Günter Schneider

Article

Schneeweis et al Cell Mol Life Sci. 2022 Dec 19;80(1):12.

Insert

Fosl1 (Fosl1 Mouse)

Use

Lentiviral

Tags

FKBP F36V tag (dTAG)

Expression

Mammalian

Mutation

Promoter

hPGK promoter

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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SQ171/pRibo-T

Plasmid

#69342

Purpose

SQ171 strain supported by pRibo-T plasmid

Depositor

Michael Jewett

Article

Orelle et al Nature. 2015 Jul 29. doi: 10.1038/nature

Insert

Ribo-T

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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SQ171fg/pRibo-T

Plasmid

#69343

Purpose

SQ171 fast growing strain supported by pRibo-T plasmid

Depositor

Michael Jewett

Article

Orelle et al Nature. 2015 Jul 29. doi: 10.1038/nature

Insert

Ribo-T

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pT7oGFP

Plasmid

#69346

Purpose

Orthogonal GFP message under T7 promoter

Depositor

Michael Jewett

Article

Orelle et al Nature. 2015 Jul 29. doi: 10.1038/nature

Insert

Super folder GFP

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a_speG_Cterm_His

Plasmid

#220483

Purpose

heterologous expression for protein purification

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

speG

Use

Tags

6xHs

Expression

Bacterial

Mutation

none

Promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_PA4114_Cterm_His

Plasmid

#220484

Purpose

heterologous expression of PA4114 for protein purification

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA4114

Use

Tags

6xHs

Expression

Bacterial

Mutation

none

Promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_PA1472_Cterm_His

Plasmid

#220485

Purpose

heterologous expression of PA1472 for protein purification

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA1472

Use

Tags

6xHs

Expression

Bacterial

Mutation

none

Promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_speG_Nterm_His

Plasmid

#220478

Purpose

heterologous expression for protein purification

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

speG

Use

Tags

6xHs

Expression

Bacterial

Mutation

none

Promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_PA4114_Nterm_His

Plasmid

#220479

Purpose

heterologous expression of PA4114 for protein purification

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA4114

Use

Tags

6xHs

Expression

Bacterial

Mutation

none

Promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge