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pE-35S-miniSdd7-nCas9-PBE-GmPPO2

Plasmid

#204855

Purpose

For cytosine base editing using miniSdd7 at PPO2 site in soybean (Agrobacterium-mediated transformation)

Depositor

Caixia Gao

Article

Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

Insert

miniSdd7-nSpCas9(D10A)-UGI-mScarlet

Use

CRISPR

Tags

Expression

Plant

Mutation

D10A in SpCas9

Promoter

35S, AtU6-26p

Available since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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MMLV-RT(dRH) for Split-PE (pEH50)

Plasmid

#190106

Purpose

Expresses MMLV-RT (from PE2) with truncated RNAse H domain (dRH) for Split-PE use

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

Insert

MMLV-RT(delta RNAseH)

Use

Tags

bpNLS

Expression

Mammalian

Mutation

mutations from RT in PE2 and truncation of RNAse …

Promoter

CMV

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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Marathon-RT tetramutant for Split-PE (pEH101)

Plasmid

#190109

Purpose

Expresses Marathon-RT tetramutant (D14R-D74R-N116K-N197R)

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

Insert

Marathon-RT

Use

Tags

bpNLS

Expression

Mammalian

Mutation

D14R, D74R, N116K, N197R

Promoter

CMV

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-p3-PE(1152)-N-term

Plasmid

#187181

Purpose

p3-PE2-N-term(1152) in AAV2 backbone (ITRs)

Depositor

Gerald Schwank

Article

Bock et al Sci Transl Med. 2022 Mar 16;14(636):eabl

Insert

PE2 from plasmid #132775

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pE-cadherin-GFP-N-WASP deltaVCA

Plasmid

#101283

Purpose

Mammalian expression of E-cadherin-GFP-N-WASP delta VCA

Depositor

Alpha Yap

Article

Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

Insert

E-Cadherin-GFP-N-WASP (Cdh1, Wasl Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

delta VCA domain

Promoter

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-p3-PE(1153-dRnH)-C-term

Plasmid

#187182

Purpose

p3-PE2-C-term(1153-dRnH) in AAV2 backbone (ITRs) targeting DNMT1

Depositor

Gerald Schwank

Article

Bock et al Sci Transl Med. 2022 Mar 16;14(636):eabl

Insert

PE2 from plasmid #132775 with shortened RnaseH domain

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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delta PE OCT4-GFP-2A-PURO transgenic reporter

Plasmid

#52380

Purpose

BAC recombineering generated construct, used as a reporter for OCT4 expression

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Inserts

GFP-2A-puro
OCT4 (POU5F1 Human)

Use

Tags

Expression

Mammalian

Mutation

deleted -1620 - -1011 upstream from OCT4 ATG

Promoter

Available since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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AAV2 for Split-PE, gRNAs + MMLV-RT(dRH)-P2A-eGFP (PKC1002)

Plasmid

#190112

Purpose

AAV2 for expression of pegRNA and ngRNA (HEKs3, CTT insertion) + MMLV-RT(dRH)-P2A-eGFP

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

Inserts

MMLV-RT(dRH)
U6-pegRNA(HEKs3, CTT ins)-H1-ngRNA(HEK s3)

Use

AAV

Tags

bpNLS and bpNLS-P2A-eGFP

Expression

Mutation

mutations from RT in PE2 and truncation of RNAse …

Promoter

EFS and U6, H1

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLN193 (SSX1p-[mK-Ex1]-[miR1-Mv3 intron]-[mK-Ex2]-BS(Pe))

Plasmid

#105210

Purpose

Module 1 - SSX1 promoter drives self-inhibiting mKate2 expression (Version 3 intron - see PMID: 29056342 for detailed information)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

SSX1p-[mK-Ex1]-[miR1-Mv3 intron]-[mK-Ex2]-BS(Pe)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLN520 (SSX1p-[mK-Ex1]-[miR1-Mv1 intron]-[mK-Ex2]-BS(Pe))

Plasmid

#105207

Purpose

Module 1 - SSX1 promoter drives self-inhibiting mKate2 expression (Version 1 intron - see PMID: 29056342 for detailed information)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

SSX1p-[mK-Ex1]-[miR1-Mv1 intron]-[mK-Ex2]-BS(Pe)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pLN189 (SSX1p-[mK-Ex1]-[miR1-Mv2 intron]-[mK-Ex2]-BS(Pe))

Plasmid

#105209

Purpose

Module 1 - SSX1 promoter drives self-inhibiting mKate2 expression (Version 2 intron - see PMID: 29056342 for detailed information)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

SSX1p-[mK-Ex1]-[miR1-Mv2 intron]-[mK-Ex2]-BS(Pe)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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TC358

Plasmid

#122613

Purpose

TRE-delta-pes-10-GFP

Depositor

Tian Chi

Article

Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

Insert

7XTRE-delta-pes-10-GFP

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pBN100

Plasmid

#35106

Depositor

John McCusker

Article

Hartzog et al Yeast. 2005 Jul 30;22(10):789-98.

Insert

caURA3PR

Use

Yeast replicating

Tags

Expression

Mutation

Promoter

Available since

Sept. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pBN42

Plasmid

#35103

Depositor

John McCusker

Article

Hartzog et al Yeast. 2005 Jul 30;22(10):789-98.

Insert

kanPR

Use

Yeast replicating

Tags

Expression

Mutation

Promoter

Available since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pPH37

Plasmid

#35102

Depositor

John McCusker

Article

Hartzog et al Yeast. 2005 Jul 30;22(10):789-98.

Insert

fcy1MX4

Use

Yeast replicating

Tags

Expression

Mutation

Promoter

Available since

April 19, 2012

Availability

Academic Institutions and Nonprofits only

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pPH36

Plasmid

#35101

Depositor

John McCusker

Article

Hartzog et al Yeast. 2005 Jul 30;22(10):789-98.

Insert

fca1MX4

Use

Yeast replicating

Tags

Expression

Mutation

Promoter

Available since

April 19, 2012

Availability

Academic Institutions and Nonprofits only

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pBN63

Plasmid

#35104

Depositor

John McCusker

Article

Hartzog et al Yeast. 2005 Jul 30;22(10):789-98.

Insert

fcy1PR

Use

Yeast replicating

Tags

Expression

Mutation

Promoter

Available since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pBN62

Plasmid

#35105

Depositor

John McCusker

Article

Hartzog et al Yeast. 2005 Jul 30;22(10):789-98.

Insert

fcy1PR

Use

Yeast replicating

Tags

Expression

Mutation

Promoter

Available since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pPH 34

Plasmid

#35098

Depositor

John McCusker

Article

Hartzog et al Yeast. 2005 Jul 30;22(10):789-98.

Insert

fca1MX3

Use

Yeast replicating

Tags

Expression

Mutation

Promoter

Available since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only

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pRSET Nucleolin

Plasmid

#13037

Depositor

Peter Newburger

Article

Wu et al J Cell Biochem. 2000 Apr . 77(3):507-16.

Insert

Nucleolin (NCL Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 5, 2006

Availability

Academic Institutions and Nonprofits only

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