We narrowed to 4,237 results for: pes;

1. pJFRC-10XUAS-FRT-STOP-FRT-myrGFP-2A-KDR::Pest

   Plasmid

   #217513

   Purpose

   Encodes a UAS controlled and Flp dependent conditional trangene of bicistronic myrGFP and KD Recombinase

   Depositor

   S. Lawrence Zipursky

   Article

   Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

   Insert

   10XUAS-FRT-STOP-FRT-myrGFP-2A-KDR::Pest
   

   Expression

   Insect

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPBJ pFOS-KTR-Gal4-PEST-tub3’ pCMV-TagBFP

   Plasmid

   #176531

   Purpose

   PiggyBAC vector expressing a FOS promoter driven "KGV" synthetic transcription factor and a CMV promoter driven BFP marker.

   Depositor

   Jared Toettcher

   Article

   Ravindran et al Cell Syst. 2022 Feb 16;13(2):131-142.e13

   Inserts

   KGV transcription factor
   TagBFP
   

   Use

   Piggybac transposase integration vector

   Tags

   ErkKTR, Gal4 DNA binding domain, PEST destabiliza…

   Expression

   Mammalian

   Available Since

   March 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJFRC-10XUAS-FRT-STOP-FRT-myrTdtomato-2A-KDR::Pest

   Plasmid

   #217514

   Purpose

   Encodes a UAS controlled and Flp dependent conditional trangene of bicistronic myrTdtomato and KD Recombinase

   Depositor

   S. Lawrence Zipursky

   Article

   Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

   Insert

   10XUAS-FRT-STOP-FRT-myrTdtomato-2A-KDR::Pest
   

   Expression

   Insect

   Available Since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJM553 ZF10x6-C 3xFLAG-NLS-DsDed-ZF1-PEST in TUPV2

   Plasmid

   #161540

   Purpose

   Inducible expression of 3xFLAG-NLS-DsDed-ZF1-PEST under the ZF10x6-C promoter

   Depositor

   Joshua Leonard

   Article

   Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

   Insert

   3xFLAG-NLS-DsRed-ZF1-PEST
   

   Use

   Synthetic Biology

   Tags

   3x-FLAG

   Expression

   Mammalian

   Promoter

   ZF10x6-C

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV-CMP-PE-V1

   Plasmid

   #202769

   Purpose

   Expresses CMP-PE-V1 in mammalian cells for prime editing

   Depositor

   Kyoungmi Kim

   Article

   Park et al Genome Biol. 2021 Jun 3;22(1):170. doi:

   Insert

   CMP-PE-V1
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PE-GsI-IIC-RT

   Plasmid

   #181808

   Purpose

   PE-GsI-IIC RT

   Depositor

   Erik Sontheimer

   Article

   Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

   Insert

   PE-GsI-IIC-RT
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PE-SUMO CRYGS Q16C

   Plasmid

   #217674

   Purpose

   For expression of SUMO tagged human gamma S crystallin with Q16C mutation to act as a biological control for ligation experiment

   Depositor

   Larry David

   Insert

   CRYGS (CRYGS Human)
   

   Tags

   6XHis and SUMO

   Expression

   Bacterial

   Mutation

   Glutamine 16 changed to Cysteine

   Promoter

   T7

   Available Since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MF84_PB-14xUAS-6x42bs-6x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA

   Plasmid

   #176628

   Purpose

   TF A self-activation cassette in MultiFate-2.3

   Depositor

   Michael Elowitz

   Article

   Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.

   Insert

   14xUAS-6x42bs-6x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA
   

   Use

   Synthetic Biology

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MF64_PB-TRE3G-6x42bs-6x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA

   Plasmid

   #176624

   Purpose

   TF B self-activation cassette in MultiFate-2.1 and MultiFate-2.3

   Depositor

   Michael Elowitz

   Article

   Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.

   Insert

   TRE3G-6x42bs-6x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA
   

   Use

   Synthetic Biology

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MF81_PB-TRE3G-12x42bs-6x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA

    Plasmid

    #176625

    Purpose

    TF A self-activation cassette in MultiFate-2.1

    Depositor

    Michael Elowitz

    Article

    Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.

    Insert

    TRE3G-12x42bs-6x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA
    

    Use

    Synthetic Biology

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MF83_PB-TRE3G-6x42bs-10x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA

    Plasmid

    #176627

    Purpose

    TF B self-activation cassette in MultiFate-2.2 and MultiFate-3

    Depositor

    Michael Elowitz

    Article

    Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.

    Insert

    TRE3G-6x42bs-10x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA
    

    Use

    Synthetic Biology

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MF85_PB-14xUAS-12x42bs-10x(ErbB2bs_ErbB2bs)-miniCMV-NLS-FKBP12F36V-ErbB2ZFR2AR39A-VP16-NLS-DHFR-IRES-mTurquoise2-PEST-BGHpA

    Plasmid

    #176629

    Purpose

    TF C self-activation cassette in MultiFate-3

    Depositor

    Michael Elowitz

    Article

    Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.

    Insert

    14xUAS-12x42bs-10x(ErbB2bs_ErbB2bs)-miniCMV-NLS-FKBP12F36V-ErbB2ZFR2AR39A-VP16-NLS-DHFR-IRES-mTurquoise2-PEST-BGHpA
    

    Use

    Synthetic Biology

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MF82_PB-TRE3G-12x42bs-10x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA

    Plasmid

    #176626

    Purpose

    TF A self-activation cassette in MultiFate-2.2 and MultiFate-3

    Depositor

    Michael Elowitz

    Article

    Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.

    Insert

    TRE3G-12x42bs-10x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA
    

    Use

    Synthetic Biology

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SpCas9 nickase for Split-PE (pJUL2497)

    Plasmid

    #190104

    Purpose

    Expresses nickase SpCas9(H840A) for Split-PE use

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    nSpCas9
    

    Tags

    XTEN-bpNLS and bpNLS

    Expression

    Mammalian

    Mutation

    H840A

    Promoter

    CMV

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TDP-REGv2 Cryptic PE-Max

    Plasmid

    #216163

    Purpose

    Expression of prime editor PEMax (Plasmid #174820) only in cells with TDP-43 loss of function. Note: It is not recommended to produce lentivirus containing TDP-REG sequences – see Depositor Comments

    Depositor

    Pietro Fratta

    Article

    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

    Insert

    PEMax for prime editing with internal cryptic exon
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Marathon-RT for Split-PE (pEH59)

    Plasmid

    #190108

    Purpose

    Expresses Marathon-RT (human codon optimization)

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    Marathon-RT
    

    Tags

    bpNLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MMLV-RT for Split-PE (pEH45)

    Plasmid

    #190105

    Purpose

    Expresses MMLV-RT (full-length, from PE2) for Split-PE use

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    MMLV-RT
    

    Tags

    bpNLS

    Expression

    Mammalian

    Mutation

    RT pentamutant from PE2 (D200N, T306K, W313F, T33…

    Promoter

    CMV

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PE-pegRNA(BG)-nsgRNA(BG)

    Plasmid

    #232727

    Purpose

    A dual-targeting vector that contains PE3 system guide RNAs against BFP (pegRNA(BG) and nsgRNA(BG)) with restriction enzyme sites for insertion of target site guide RNAS (pegRNAs(TS) and nsgRNA(TS)).

    Depositor

    David Brafman

    Article

    Frisch et al Mol Ther Nucleic Acids. 2023 Jul 17;33:4

    Insert

    pegRNA(BG), nsgRNA(BG), pegRNAs(TS), nsgRNA(TS)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAV1 for Split-PE, SpCas9 nickase (PKC1001)

    Plasmid

    #190111

    Purpose

    AAV1 for expression of nickase SpCas9(H840A) for Split-PE use

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    nSpCas9
    

    Use

    AAV

    Tags

    bpNLS

    Mutation

    H840A

    Promoter

    EF1a

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HERV-Kcon-RT for Split-PE (pJUL3120)

    Plasmid

    #190107

    Purpose

    Expresses HERV-Kcon RT (human codon optimization)

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    HERV-Kcon-RT
    

    Tags

    bpNLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge