We narrowed to 17,900 results for: RET

Showing: 5161 - 5200 of 17900 results

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pGEX SYT1 C2A

Plasmid

#195710

Purpose

Bacterial expression plasmid encoding GST-tagged SYT1 C2A domain

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt1 (Syt1 Rat)

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 96-264 of mouse SYT1

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SYT1 C2B

Plasmid

#195711

Purpose

Bacterial expression plasmid encoding GST-tagged SYT1 C2B domain

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt1 (Syt1 Rat)

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 248-421 of mouse SYT1

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SYT1 C2AB

Plasmid

#195712

Purpose

Bacterial expression plasmid encoding GST-tagged SYT1 C2AB domain

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt1 (Syt1 Rat)

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 96-421 of mouse SYT1

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pET (SUMO)-Syt9

Plasmid

#195713

Purpose

Bacterial expression plasmid encoding SUMO-tagged full-length Syt9

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Rat)

Tags

GST-SUMO

Expression

Bacterial

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-RO1.7-dnHIF

Plasmid

#206343

Purpose

AAV plasmid expressing dominant negative HIF1A in photoreceptors

Depositor

Connie Cepko

Article

Xue et al Elife. 2021 Apr 13;10. pii: 66240. doi:

Insert

dominant negative hif1a

Use

AAV

Mutation

dominant negative HIF1A

Promoter

1.7kb human red opsin

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-RedO-shNC

Plasmid

#206356

Purpose

AAV plasmid expressing non-targeting control shRNA in photoreceptors

Depositor

Connie Cepko

Article

Xue et al Elife. 2024 May 10;12:RP90749. doi: 10.7

Insert

Non-targeting control shRNA

Use

AAV

Promoter

human red opsin

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pDGE1176

Plasmid

#202774

Purpose

CRISPR-KO in Arabidopsis thaliana

Depositor

Johannes Stuttmann

Article

Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

Inserts

FAST marker
pRPS5a:zCas9_tNbEU
ccdB cassette (BsaI-excisable)

Use

CRISPR

Expression

Plant

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pDGE1177

Plasmid

#202775

Purpose

CRISPR-KO with TRE2 exonuclease co-expression in Arabidopsis thaliana

Depositor

Johannes Stuttmann

Article

Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

Inserts

FAST marker
pRPS5a:TREX2-zCas9_tNbEU
ccdB cassette (BsaI-excisable)

Use

CRISPR

Expression

Plant

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pUCSh2-mRFP

Plasmid

#170982

Purpose

Encodes sgRNA scaffold with mRFP in place of target and Kanamycin resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

mRFP

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBMN BFP

Plasmid

#188644

Purpose

This is the Lazarou lab's standard retrovirus vector backbone for expression of BFP-tag fusions onto the N-terminus of cloned genes

Depositor

Michael Lazarou

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Type

Empty backbone

Use

Retroviral

Tags

BFP

Expression

Mammalian

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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p5’UTR-ORF1

Plasmid

#190566

Purpose

Holding vector containing the contiguous 5’UTR and ORF1 regions of human L1 (L1.3) to facilitate subcloning of ORF1 mutants into the full-length L1 reporter pRTC2-puro (Cook et al., 2015).

Depositor

Anthony Furano

Article

Cook et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Contiguous regions of the human L1 (L1.3) 5’UTR and ORF1

Use

For dna propagation only

Expression

Bacterial

Promoter

N/A

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pACT2-μ4

Plasmid

#198176

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-4 μ4 fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

Insert

AP-4 μ4

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Promoter

ADH1

Available Since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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pET30_Halotag_K73T_Jdomain

Plasmid

#183693

Purpose

Bacterial expression of ER-targeted metastable Halotag/folding sensor (HaloK73T) fused with J domain

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Insert

ER Halo(K73T)-J domain

Expression

Bacterial

Mutation

K73T in Halotag

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pACT2-μ2

Plasmid

#198173

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-2 μ2 fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

Insert

AP-2 μ2

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Promoter

ADH1

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pPW3757 : CMVd3-ERmembrane-2xHaloTag-KKMP

Plasmid

#185679

Purpose

Low-level transient expression of two tandem HaloTag proteins targeted to the ER membrane.

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERmembrane-2xHaloTag-KKMP

Expression

Mammalian

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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UG75-PAH1-3HA

Plasmid

#184761

Purpose

PAH1 overexpression. Promotes the formation of diacylglycerol from phosphatidate. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

PAH1 (PAH1 Budding Yeast)

Tags

3HA

Expression

Yeast

Promoter

PAH1

Available Since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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UG75-CDS1-3HA

Plasmid

#184760

Purpose

CDS1 overexpression. Diverts glycerolipid synthesis pathway towards phospholipids. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

CDS1 (CDS1 Budding Yeast)

Tags

3HA

Expression

Yeast

Promoter

CDS1

Available Since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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UG75-DGK1-3HA

Plasmid

#184759

Purpose

DGK1 overexpression. Promotes the formation of phosphatidate from diacylglycerol. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

DGK1 (DGK1 Budding Yeast)

Tags

3HA

Expression

Yeast

Promoter

DGK1

Available Since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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OM14-2EGFP-UG74-ter

Plasmid

#184758

Purpose

Marker for yeast mitochondria. Integration of 2xGFP tag at OM14 C terminus. Uses antibiotic resistance marker natMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

OM14 (OM14 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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SEC7-2EGFP-ter-NAT

Plasmid

#184764

Purpose

Marker for yeast late Golgi/early endosome . Integration of 2xGFP tag at SEC7 C terminus. Uses antibiotic resistance marker natMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

SEC7 (SEC7 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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CIT1-2EGFP-ter-URA3

Plasmid

#184767

Purpose

Marker for yeast mitochondria. Integration of 2xGFP tag at CIT1 C terminus. Uses auxotrophic marker URA3(Candida albicans).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

CIT1 (CIT1 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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ACH1-2EGFP-ter-URA3

Plasmid

#184769

Purpose

Marker for yeast mitochondria. Integration of 2xGFP tag at ACH1 C terminus. Uses auxotrophic marker URA3(Candida albicans).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

ACH1 (ACH1 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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AFG3-2EGFP-ter-URA3

Plasmid

#184770

Purpose

Marker for yeast mitochondria. Integration of 2xGFP tag at AFG3 C terminus. Uses auxotrophic marker URA3(Candida albicans).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

AFG3 (AFG3 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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SYS1-2EGFP-ter-URA3

Plasmid

#184771

Purpose

Marker for yeast early Golgi. Integration of 2xGFP tag at SYS1 C terminus. Uses auxotrophic marker URA3(Candida albicans).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

SYS1 (SYS1 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Rtn2b-R60f-BFP

Plasmid

#186608

Purpose

Encodes human Reticulon 2 disease mutant R60f fused to BFP

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 mutant R60f (RTN2 Human)

Tags

BFP

Expression

Mammalian

Mutation

c.178insC (R60f)

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-Rtn2b-mNeon-TurboID

Plasmid

#186612

Purpose

Third generation lentiviral vector expressing full-length Reticulon 2 fused to mNeonGreen and V5-TurboID

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 isoform B (RTN2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Rtn2b-NTD1-271-mNeon

Plasmid

#186601

Purpose

Encodes human Reticulon 2 isoform B N-terminal domain aa 1 to 271 fused to mNeonGreen

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 isoform B amino acids 1 to 271 (RTN2 Human)

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Rtn2b-NTD1-135-mNeon

Plasmid

#186602

Purpose

Encodes human Reticulon 2 isoform B N-terminal domain aa 1 to 135 fused to mNeonGreen

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 isoform B amino acids 1 to 135 (RTN2 Human)

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Rtn2b-NTD1-60-mNeon

Plasmid

#186603

Purpose

Encodes human Reticulon 2 isoform B N-terminal domain aa 1 to 60 fused to mNeonGreen

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 isoform B amino acids 1 to 60 (RTN2 Human)

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
SEC7-2Katushka-ter-Hygromycin

Plasmid

#184772

Purpose

Red marker for yeast late Golgi/early endosome. Integration of 2xKatushka tag at SEC7 C terminus. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

SEC7 (SEC7 Budding Yeast)

Tags

2Katushka

Expression

Yeast

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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PKC1-2GFP-ter-TRP1

Plasmid

#184773

Purpose

Integration of 2xGFP tag at PKC1 C terminus. Uses auxotrophic marker TRP1(Kluyveromyces lactis).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

PKC1 (PKC1 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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UAS-GFP::NMN-Deamidase

Plasmid

#187868

Purpose

Gal4/UAS expression of GFP::NMN-Deamidase

Depositor

Lukas Neukomm

Article

Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

Insert

NMN-Deamidase

Tags

GFP

Expression

Insect

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-BLZinCh-P40

Plasmid

#188057

Purpose

Expression of BLZinCh-P40, a dual read-out BRET-FRET sensor for intracellular Zn2+ imaging, in mammalian cells

Depositor

Maarten Merkx

Article

Michielsen et al ACS Chem Biol. 2022 Jun 17;17(6):1567-15

Insert

BLZinCh-P40

Expression

Mammalian

Promoter

CMV promotor

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-BLZinCh-P50

Plasmid

#188058

Purpose

Expression of BLZinCh-P50, a dual read-out BRET-FRET sensor for intracellular Zn2+ imaging, in mammalian cells

Depositor

Maarten Merkx

Article

Michielsen et al ACS Chem Biol. 2022 Jun 17;17(6):1567-15

Insert

BLZinCh-P50

Expression

Mammalian

Promoter

CMV promotor

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCL.103

Plasmid

#184988

Purpose

Express Cas9-P2A-Eco1RT

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Cas9-P2A-Eco1RT

Tags

SV40NLS

Expression

Yeast

Mutation

human codon optimized RT

Promoter

Gal1-10

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCL.031

Plasmid

#184968

Purpose

Test effect of extending -Eco2 a1/a2 in yeast

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco2: RT and ncRNA(extended), a1/a2 length: 29

Expression

Yeast

Mutation

human codon optimized RT, a1/a2 length extended t…

Promoter

Gal7

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCL.027

Plasmid

#184964

Purpose

Express -Eco1 RT and ncRNA in yeast

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1: RT and ncRNA(wt), a1/a2 length: 12

Expression

Yeast

Mutation

human codon optimized RT

Promoter

Gal7

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCL.72

Plasmid

#184984

Purpose

Express Cas9/-Eco1 RT fusion, using linker1

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Cas9-fusion_linker1-Eco1RT

Tags

SV40NLS

Expression

Yeast

Mutation

human codon optimized RT

Promoter

Gal1-10

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCL.95

Plasmid

#184986

Purpose

Express Cas9/-Eco1 RT fusion, using linker2

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Cas9-fusion_linker2-Eco1RT

Tags

SV40NLS

Expression

Yeast

Mutation

human codon optimized RT

Promoter

Gal1-10

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCL.102

Plasmid

#184987

Purpose

Express -Eco1 RT-P2A-Cas9

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1RT-P2A-Cas9

Tags

SV40NLS

Expression

Yeast

Mutation

human codon optimized RT

Promoter

Gal1-10

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 17,900 results for: RET